Activation mechanism of a bacterial two-componet system by an outer membrane lipoprotein sensing cell envelope stresses

• Project/Area Number: 22570001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Saitama University
• Principal Investigator: HARA Hiroshi 埼玉大学, 大学院・理工学研究科, 准教授 (00173071)
• Co-Investigator(Kenkyū-buntansha): MATSUMOTO Kouji 埼玉大学, 大学院・理工学研究科, 教授 (00119140) ; MATSUOKA Satoshi 埼玉大学, 大学院・理工学研究科, 助教 (90509283)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 外膜リポタンパク質 / 二成分制御系 / 細胞表層ストレス / 細菌リポタンパク質 / 機能ドメイン構造 / タンパク質間相互作用

Research Abstract

The outer membrane lipoprotein RcsF is an essential component of the Rcs phosphorelay signal transduction system in Escherichia coli. It senses stresses imposed on the cell envelope and conveys the information to histidine kinase RcsC in the cytoplasmic membrane. It is suggested that RcsF functions as a ligand for RcsC in activating the system. RcsF has a region rich in proline and basic residues near the N terminus. This region is likely to play an important role in regulation of the function of RcsF in activating the Rcs system.

Research Products

• [Journal Article] Induction of extracytoplasmic function sigma factors in Bacillus subtiliscells with defects in lipoteichoic acid synthesis (2013)
  • Author(s): Hashimoto, M., T. Seki, S. Matsuoka, H. Hara, K. Asai, Y. Sadaie, and K. Matsumoto
  • Journal Title: Microbiology Volume: 159(1) Issue: Pt_1 Pages: 23-35
  • DOI: 10.1099/mic.0.063420-0
  • Peer Reviewed
• [Journal Article] Activation of the Cpx phosphorelay signal transduction system in acidic phospholipid-deficient pgsA mutant cells of Escherichia coli (2012)
  • Author(s): Aya Itou, Kouji Matsumoto, and Hiroshi Hara.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 421(2) Pages: 296-300
  • Peer Reviewed
• [Journal Article] Exploring the relationship between lipoprotein mislocalization and activation of the Rcs signal transduction system in Escherichia coli (2012)
  • Author(s): Yasuhiro Shiba, Hiroyoshi Miyagawa, Hideki Nagahama, Kenji Matsumoto, Daitetsu Kondo, Satoshi Matsuoka, Kouji Matsumoto, and Hiroshi Hara
  • Journal Title: Microbiology Volume: 158(5) Pages: 1238-1248
  • Peer Reviewed
• [Journal Article] Expression and Localization of Two SecA Homologs in the Unicellular Red Alga Cyanidioschyzon merolae (2012)
  • Author(s): Yosuke Koyama, Yasuko Kaneko, Satoshi Matsuoka, Kouji Matsumoto, Hiroshi Hara, and Niji Ohta
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 2 Pages: 417-422
  • DOI: 10.1271/bbb.110833
  • NAID: 10030400439
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Exploring the relationship between lipoprotein mislocalisation and activation of the Res signal transduction system in Escherichia coli (2012)
  • Author(s): Shiba, Miyagawa, Nagahama, Matsumoto, Kondo, Matsuoka, Matsumoto, Hara
  • Journal Title: Microbiology Volume: 158 Issue: 5 Pages: 1238-1248
  • DOI: 10.1099/mic.0.056945-0
  • Peer Reviewed
• [Journal Article] Activation of the Cpx phosphorelay signal transduction system in acidic phospholipid-deficient pgsA mutant cells of Escherichia coli (2012)
  • Author(s): Aya Itou, Kouji Matsumoto, Hiroshi Hara
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 421 Issue: 2 Pages: 296-300
  • DOI: 10.1016/j.bbrc.2012.04.003
  • Peer Reviewed
• [Journal Article] ストレス応答ECFシグマ因子を介した枯草菌におけるリポテイコ酸の恒常性の維持． (2012)
  • Author(s): 橋本 理尋，関 貴洋，松岡 聡，原 弘志，朝井 計，定家 義人，松本 幸次
  • Journal Title: 脂質生化学研究 Volume: 54 Pages: 138-141
  • NAID: 10030581402
• [Journal Article] Expression and localization of two SecA homologs in the unicelllular red alga Cyanidioschyzon merolae (2012)
  • Author(s): Yosuke Koyama, Yasuko Kaneko, Satoshi Matsuoka, Kouji Matsumoto, Hiroshi Hara, Niji Ohta
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Pages: 417-422
  • Peer Reviewed
• [Journal Article] The Bacillus subtilis essential gene dgkB is dispensable in mutants with defective lipoteichoic acid synthesis (2011)
  • Author(s): Satoshi Matsuoka, Michihiro Hashimoto, Yusuke Kamiya, Takeshi Miyazawa, Kazuki Ishikawa, Hiroshi Hara, Kouji Matsumoto
  • Journal Title: Genes and Genetic Systems Volume: 86 Pages: 365-376
  • NAID: 10030210125
  • Peer Reviewed
• [Journal Article] Abnormal morphology of Bacillus subtilis ugtP mutant cells lacking glucolipids (2011)
  • Author(s): Satoshi Matsuoka, Minako Chiba, Yu Tanimura, Michihiro Hashimoto, Hiroshi Hara, Kouji Matsumoto
  • Journal Title: Genes and Genetic Systems Volume: 86 Pages: 295-304
  • NAID: 10030209806
  • Peer Reviewed
• [Journal Article] Characterization of the Nuclear- and Plastid-Encoded secA-Homologous Genes in the Unicellular Red Alga Cyanidioschyzon merolae (2011)
  • Author(s): Yosuke Koyama, Koji Takimoto, Asuka Kojima, Kei Asai, Satoshi Matsuoka, Toshiaki Mitsui, Kouji Matsumoto, Hiroshi Hara, Niji Ohta
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 75 Issue: 10 Pages: 2073-2078
  • DOI: 10.1271/bbb.110338
  • NAID: 10029873431
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Involvement of σ^S accumulation in repression of the flhDC operon in acidic phospholipid-deficient mutants of Escherichia coli. (2010)
  • Author(s): Junji Uchiyama, et al.
  • Journal Title: Microbiology Volume: Vol.156 Pages: 1650-1660
  • Peer Reviewed
• [Journal Article] Accumulation of σ^S due to enhanced synthesis and decreased degradation in acidic phospholipid-deficient Escherichia coil cells. (2010)
  • Author(s): Junji Uchiyama, et al.
  • Journal Title: FEMS Microbiology Letters Volume: Vol.307 Pages: 120-127
  • Peer Reviewed
• [Presentation] Influence of glucolipids on regulation of extracytoplasmic function sigma factors. (2012)
  • Author(s): Satoshi Matsuoka, Hiroshi Hara, and Kouji Matsumoto
  • Organizer: The 12th Asian-Pacific Conference on Transcription
  • Place of Presentation: Seogwippo KAL Hotel, Jeju Island, Korea
• [Presentation] Induction of extracyltoplasmic function sigma factor σM in Bacilllus subtilis cells laking glucolipids. (2012)
  • Author(s): Takahiro Seki, Michihiro Hashimoto, Satoshi Matsuoka, Hiroshi Hara, and Kouji Matsumoto
  • Organizer: The 12th Asian-Pacific Conference on Transcription
  • Place of Presentation: Seogwippo KAL Hotel, Jeju Island, Korea
• [Presentation] ストレス応答ECFシグマ因子を介した枯草菌におけるリポテイコ酸の恒常性の維持． (2012)
  • Author(s): 橋本 理尋，関 貴洋，松岡 聡，原 弘志，朝井 計，定家 義人，松本 幸次
  • Organizer: 日本脂質生化学会
  • Place of Presentation: 九州大学医学部百年講堂(福岡)
• [Presentation] ECFシグマ因子の制御における糖脂質の影響． (2012)
  • Author(s): 松岡 聡，原 弘志，松本 幸次
  • Organizer: 平成24年度グラム陽性菌ゲノム機能会議
  • Place of Presentation: 焼津グランドホテル(焼津)
• [Presentation] ECFシグマ因子の制御における糖脂質の影響． (2012)
  • Author(s): 松岡 聡，原 弘志，松本 幸次
  • Organizer: 日本遺伝学会第84回大会
  • Place of Presentation: 九州大学医学部百年講堂(福岡)
• [Presentation] 大腸菌酸性リン脂質欠損によるσS蓄積機構の解析． (2012)
  • Author(s): 及川 達巳，佐々木 優，内山 順爾，木村 絵里，松岡 聡，原 弘志，松本 幸次
  • Organizer: 日本遺伝学会第84回大会
  • Place of Presentation: 九州大学医学部百年講堂(福岡)
• [Presentation] 糖脂質を欠損する枯草菌細胞におけるECFシグマ因子の活性化の解析． (2012)
  • Author(s): 関 貴洋，橋本 理尋，松岡 聡，原 弘志，松本 幸次
  • Organizer: 日本遺伝学会第84回大会
  • Place of Presentation: 九州大学医学部百年講堂(福岡)
• [Presentation] リポテイコ酸を欠損する枯草菌細胞におけるストレス応答ECFシグマ因子の活性化． (2012)
  • Author(s): 橋本 理尋，関 貴洋，松岡 聡，原 弘志，朝井 計，定家 義人，松本 幸次
  • Organizer: 日本遺伝学会第84回大会
  • Place of Presentation: 九州大学医学部百年講堂(福岡)
• [Presentation] 枯草菌MinDのC末端に存在する2つの両親媒性α-helixの役割の解析． (2012)
  • Author(s): 古川 祐吾，唐澤 伸明，松島 若菜，石川 一輝，松岡 聡，原 弘志，松本 幸次
  • Organizer: 日本遺伝学会第84回大会
  • Place of Presentation: 九州大学医学部百年講堂(福岡)
• [Presentation] 枯草菌の細胞形態維持に関わるUgtPの機能解析． (2012)
  • Author(s): 西野 有紀，宮松 沙織，千葉 美奈子，松岡 聡，原 弘志，松本 幸次
  • Organizer: 日本遺伝学会第84回大会
  • Place of Presentation: 九州大学医学部百年講堂(福岡)
• [Presentation] 葉緑体Sec translocaseにおける2種のタンパク質膜透過駆動因子SecA ATPaseの機能 (2011)
  • Author(s): 小山陽亮, 小嶋明日香, 小黒愛子, 坂本琴美, 滝元宏治, 松岡聡, 朝井計, 金子康子, 三ツ井敏明, 松本幸次, 原弘志, 太田に
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-15
• [Presentation] 枯草菌MinDの隔壁への局在機構の解析 (2011)
  • Author(s): 松島若菜, 石川一輝, 松岡聡, 原弘志, 松本幸次
  • Organizer: 日本遺伝学会
  • Place of Presentation: 京都大学農学部総合館
  • Year and Date: 2011-09-22
• [Presentation] 枯草菌の細胞形態維持に関わるUgtPの機能解明 (2011)
  • Author(s): 宮松沙織, 千葉美奈子, 松岡聡, 原弘志, 松本幸次
  • Organizer: 日本遺伝学会
  • Place of Presentation: 京都大学農学部総合館
  • Year and Date: 2011-09-22
• [Presentation] 異種細菌由来糖脂質合成酵素遺伝子による枯草菌糖脂質欠損株の形態異常相補の試み (2011)
  • Author(s): 松岡聡, 原弘志, 松本幸次
  • Organizer: 日本遺伝学会
  • Place of Presentation: 京都大学農学部総合館
  • Year and Date: 2011-09-22
• [Presentation] 枯草菌における細胞表層負電荷の減少によるECFシグマ因子の誘導とハウスキーピングシグマ因子の低下 (2011)
  • Author(s): 橋本理尋, 谷村遊, 神谷雄介, 松岡聡, 原弘志, 朝井計, 定家義人, 松本幸次
  • Organizer: 日本遺伝学会
  • Place of Presentation: 京都大学農学部総合館(招待講演)
  • Year and Date: 2011-09-21
• [Presentation] Clostridium cellulovoransのHbpAによるセルラーゼ活性亢進作用の解析 (2011)
  • Author(s): 峯島良太, 小穴秋弓, 松岡聡, 原弘志, 松本幸次
  • Organizer: 日本遺伝学会
  • Place of Presentation: 京都大学農学部総合館
  • Year and Date: 2011-09-20
• [Presentation] 植物細胞における2つのタンパク質膜透過駆動因子SecA ATPaseの機能と生理的意義 (2011)
  • Author(s): 小山陽亮, 小嶋明日香, 小黒愛子, 滝元宏治, 松岡聡, 原弘志, 松本幸次, 太田にじ
  • Organizer: 日本遺伝学会
  • Place of Presentation: 京都大学農学部総合館
  • Year and Date: 2011-09-20
• [Presentation] 大腸菌酸性リン脂質欠損株におけるσS蓄積機構の解析 (2011)
  • Author(s): 及川達巳, 佐々木優, 内山純爾, 松岡聡, 原弘志, 松本幸次
  • Organizer: 日本遺伝学会
  • Place of Presentation: 京都大学農学部総合館
  • Year and Date: 2011-09-20
• [Presentation] Cell surface stress caused by defecive lipoteichoic acid in Bacillus subtilis (2011)
  • Author(s): Michihiro Hashimoto, Satoshi Matuoka, Hiroshi Hara, Kei Asai, Yoshito Sadaie, Kouji Matsumto
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo Convention Center
  • Year and Date: 2011-09-06
• [Presentation] Analysis of the mechanism of the septal localization of MinD in Bacillus subtilis (2011)
  • Author(s): Kazuki Ishikawa, Wakana Matsushima, Satoshi Matuoka, Hiroshi Hara, Kouji Matsumto
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo Convention Center
  • Year and Date: 2011-09-06
• [Presentation] Characterization of Bacillus subtilis glucolipid-lacking cells (2011)
  • Author(s): Satoshi Matuoka, Minako Chiba, Michihiro Hashimoto, Yu Tanimura, Kei Asai, Hiroshi Hara, Kouji Matsumto
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo Convention Center
  • Year and Date: 2011-09-06
• [Presentation] 外膜リポタンパク質RcsFの二成分制御系活性制御に働くPmline-Rich Regionの機能領域の特定 (2010)
  • Author(s): 梅川満, 他
  • Organizer: 日本遺伝学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2010-09-21
• [Presentation] 外膜リポタンパク質RcsFの二成分制御系活性制御に働くProline-Rich Regionの機能領域の特定 (2010)
  • Author(s): 梅川満,中村達郎,宮川宏義,松岡聡,原弘志,松本幸次
  • Organizer: 日本遺伝学会第82回大会.北海道大学高等教育機能開発総合センター
  • Place of Presentation: 札幌
• [Presentation] ECFシグマ因子の制御における糖脂質の影響．
  • Author(s): 松岡 聡，原 弘志，松本 幸次
  • Organizer: 第11回微生物研究会
  • Place of Presentation: 東京大学弥生講堂一条ホール(東京)
• [Presentation] 大腸菌酸性リン脂質欠損によるσS蓄積機構の解析．
  • Author(s): 及川 達巳，佐々木 優，内山 順爾，木村 絵里，松岡 聡，原 弘志，松本 幸次
  • Organizer: 第11回微生物研究会
  • Place of Presentation: 東京大学弥生講堂一条ホール(東京)
• [Presentation] 糖脂質を欠損する枯草菌細胞におけるECFシグマ因子の活性化の解析．
  • Author(s): 関 貴洋，橋本 理尋，松岡 聡，原 弘志，松本 幸次
  • Organizer: 第11回微生物研究会
  • Place of Presentation: 東京大学弥生講堂一条ホール(東京)
• [Presentation] 枯草菌の細胞形態維持に関わるUgtPの機能解析．
  • Author(s): 西野 有紀，宮松 沙織，千葉 美奈子，松岡 聡，原 弘志，松本 幸次
  • Organizer: 第11回微生物研究会
  • Place of Presentation: 東京大学弥生講堂一条ホール(東京)
• [Presentation] 枯草菌MinDのC末端に存在する2つの両親媒性α-helixの役割の解析．
  • Author(s): 古川 祐吾，唐澤 伸明，松島 若菜，石川 一輝，松岡 聡，原 弘志，松本 幸次
  • Organizer: 第11回微生物研究会
  • Place of Presentation: 東京大学弥生講堂一条ホール(東京)
• [Book] In Yoshito Sadaie and Kouji Matsumoto (Eds.), Escherichia coli and Bacillus subtilis: The Frontiers of Molecular Microbiology Revisited. Research Signpost, Kerala, India. (2012)
  • Author(s): Kouji Matsumoto, Satoshi Matsuoka and Hiroshi Hara
  • Publisher: Membranes and lipids