| Project/Area Number |
22570003
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Shinshu University |
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Principal Investigator |
ITOH Tateo 信州大学, 理学部, 特任教授 (40051817)
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| Research Collaborator |
ITOU Hiroshi 国立遺伝学研究所, 構造生物学センター, 助教 (10390626)
YAGURA Masaru 国立遺伝学研究所, 細菌遺伝学部門, 研究員 (00455205)
NEGISHI Takehiko 信州大学, 工学系研究科, 大学院・学生
TAKAHASHI Seiya 信州大学, 理学部, 学部学生
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 遺伝学 / 酵素 / 核酸 / 細菌 / プラスミド / DNA複製 / プライマーRNA / 複製開始蛋白質 / プーイマーRNA |
|---|
| Research Abstract |
DNAcontaining a set of genes is a blue print with instructions for all the organisms and its precise copy must be transferred from a parent to an offspring. In this research we wanted to know how copying of DNA starts by using a plasmid which is often used as a DNA carrier in the gene cloning. The structure and function of a protein which serves as a switch to start copying and also those of a site on the plasmid where the switch works were studied.
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