| Project/Area Number |
22570004
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Josai University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKAZAKI Mari 城西大学, 薬学部, 准教授 (50272901)
KAMIUCHI Shinnya 城西大学, 薬学部, 助教 (80433647)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | エピジェネティクス / クロマチン / ヒストン / 核マトリックス / 酸化ストレス / プロモーター / Ｐ－糖たんぱく質 / ヒストン修飾 / matrix attachment region / ゲノム / P-糖蛋白質 / mdr1 gene / mdrl gene |
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| Research Abstract |
First, we examined the location of putative MARs on the Mdr1b gene containing 10 kb upstream and downstream regions from the transcriptional start site and end of the gene using the genomatix program for MARFinder and SMARTest. Seven MARs (MAR -1 to -7; from upstream to downstream of the gene (All MARs were located in intron.) were identified on the rat Mdr1b gene. An advanced bend structure was confirmed at MAR-2 and MAR-5. Matrix binding assay showed that all MARs existed in the matrix-associated fraction. Moreover, MARs were not affected in population by hydrogen peroxide treatment. In the second study, we showed that the interaction of transcription factor, NF- B and MAR binding protein, matrin 3 increased by oxidative stress, and matrin 3 associated with the NF- B binding site of Mdr1b gene, and suggest that NF- B and matrin 3 complex were mediated for Mdr1b gene expression with the NF- B binding site of Mdr1b gene
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