Spatiotemporal alteration of chromatin structure at promoter regions
Project/Area Number |
22570006
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Genetics/Genome dynamics
|
Research Institution | Fujita Health University |
Principal Investigator |
ISHIHARA Naoe 藤田保健衛生大学, 医学部, 研究員 (50571791)
|
Co-Investigator(Kenkyū-buntansha) |
ISHIHARA Satoru 藤田保健衛生大学, 医学部, 講師 (00300723)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | アロマターゼ / クロマチン / ヒストン / プロモーター / 転写調節 |
Research Abstract |
We analyzed chromatin structure responsible to the alternativepromoter usage of the aromatase gene (CYP19A1), which is transcribed from three majorpromoters. Our novel method using sedimentation velocity centrifugation revealed thatworking promoters were exclusively composed of open chromatin. In contrast, therecruitment of trimethylation of histone H3 lysine 27, which is known as a negative markfor transcription, was observed around unused promoters, suggesting that polycombproteins repressed the promoters selectively.
|
Report
(4 results)
Research Products
(18 results)
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[Journal Article] The roleof IL-15 in activating STAT5 and fine- tuning IL-17A production in CD4 T lymphocytes2012
Author(s)
Pandiyan,P., Yang,XP., Saravanamuthu, SS., Zheng,L., Ishihara,S., O'Shea, JJ., and Lenardo, MJ
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Journal Title
J. Immunol
Volume: 189
Issue: 9
Pages: 4237-4246
DOI
Related Report
Peer Reviewed
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[Journal Article] Complex cell cycle abnor- malities caused by human T-lympho-tropic virus type 1 Tax.2011
Author(s)
Yang, L., Kotomura, N., Ho,YK., Zhi, H., Bixler, S., Schell, MJ.,and Giam,CZ
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Journal Title
J. Virol
Volume: 85
Issue: 6
Pages: 3001-3009
DOI
Related Report
Peer Reviewed
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