Spatiotemporal alteration of chromatin structure at promoter regions

• Project/Area Number: 22570006
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Fujita Health University
• Principal Investigator: ISHIHARA Naoe 藤田保健衛生大学, 医学部, 研究員 (50571791)
• Co-Investigator(Kenkyū-buntansha): ISHIHARA Satoru 藤田保健衛生大学, 医学部, 講師 (00300723)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: アロマターゼ / クロマチン / ヒストン / プロモーター / 転写調節

Research Abstract

We analyzed chromatin structure responsible to the alternativepromoter usage of the aromatase gene (CYP19A1), which is transcribed from three majorpromoters. Our novel method using sedimentation velocity centrifugation revealed thatworking promoters were exclusively composed of open chromatin. In contrast, therecruitment of trimethylation of histone H3 lysine 27, which is known as a negative markfor transcription, was observed around unused promoters, suggesting that polycombproteins repressed the promoters selectively.

Research Products

• [Journal Article] The roleof IL-15 in activating STAT5 and fine- tuning IL-17A production in CD4 T lymphocytes (2012)
  • Author(s): Pandiyan,P., Yang,XP., Saravanamuthu, SS., Zheng,L., Ishihara,S., O'Shea, JJ., and Lenardo, MJ
  • Journal Title: J. Immunol Volume: 189 Issue: 9 Pages: 4237-4246
  • DOI: 10.4049/jimmunol.1201476
  • Peer Reviewed
• [Journal Article] Complex cell cycle abnor- malities caused by human T-lympho-tropic virus type 1 Tax. (2011)
  • Author(s): Yang, L., Kotomura, N., Ho,YK., Zhi, H., Bixler, S., Schell, MJ.,and Giam,CZ
  • Journal Title: J. Virol Volume: 85 Issue: 6 Pages: 3001-3009
  • DOI: 10.1128/jvi.00086-10
  • Peer Reviewed
• [Journal Article] Two-step binding of transcription factors causes sequential chromatin structural changes at the activated IL-2 promoter (2011)
  • Author(s): Ishihara, S. and Schwartz, RH
  • Journal Title: J. Immunol Volume: 187 Issue: 6 Pages: 3292-3299
  • DOI: 10.4049/jimmunol.1003173
  • Peer Reviewed
• [Journal Article] Two-step binding of transcription factors causes sequential chromatin structural changes at the activated IL-2 promoter (2011)
  • Author(s): Satoru Ishihara
  • Journal Title: The Journal of Immunology Volume: 187 Pages: 3292-3299
  • Peer Reviewed
• [Journal Article] A new fractionation assay,based on the size of formaldehyde crosslinked, mildly sheared chroma-tin, delineates the chromatin struc-ture at promoter regions (2010)
  • Author(s): Ishihara, S., Varma, R., and Schwartz,RH
  • Journal Title: Nucleic Acids Res Volume: 38 Issue: 11 Pages: e124-e124
  • DOI: 10.1093/nar/gkq203
  • Peer Reviewed
• [Presentation] The chromatin structure responsible for the degree of the promoter activity (2012)
  • Author(s): 琴村直恵,原田信広,石原悟
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] CYP19遺伝子のプロモーター選択機構:密なクロマチン構造でプロモーターを選択的に抑制する (2012)
  • Author(s): 石原悟,琴村直恵,原田信広
  • Organizer: 第6回日本エピジェネティクス研究会年会
  • Place of Presentation: 東京
• [Presentation] CYP19遺伝子のプロモーター選択機構：密なクロマチン構造でプロモーターを選択的に抑制する (2012)
  • Author(s): 石原悟、琴村直恵、原田信広
  • Organizer: 第6回日本エピジェネティクス研究会年会
  • Place of Presentation: 東京
• [Presentation] The chromatin structure responsible for the degree of the promoter activity. (2012)
  • Author(s): Kotomura, N., Harada, N., Ishihara, S.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] The contribution of histone modifications to alternative promoter usage of the human CYP19 gene (2011)
  • Author(s): 石原悟
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
  • Year and Date: 2011-12-16
• [Presentation] ヒストンH3K27のトリメチル化によるアロマターゼ遺伝子プロモーターの選択的不活性化 (2011)
  • Author(s): 石原悟,琴村直恵,西芳寛,野村政壽,柳瀬敏彦,原田信広
  • Organizer: 第19回日本ステロイドホルモン学会学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2011-11-26
• [Presentation] ヒストンH3K27のトリメチル化によるアロマターゼ遺伝子プロモーターの選択的不活性化 (2011)
  • Author(s): 石原悟
  • Organizer: 第19回日本ステロイドホルモン学会学術集会
  • Place of Presentation: 福岡大学病院メディカルホール(福岡県福岡市)
  • Year and Date: 2011-11-26
• [Presentation] Condensed Chromatin Marked by K27-Trimethylated Histone H3 Determines Alternative Promoter Usage of the CYP19 Gene (2011)
  • Author(s): 石原悟
  • Organizer: 第5回日本エピジェネティクス研究会年会
  • Place of Presentation: KKRホテル熊本(熊本県熊本市)
  • Year and Date: 2011-05-19
• [Presentation] Thecontribution of histone modifica-tions to alternative promoter usageof the human CYP19 gene. (2011)
  • Author(s): 琴村直恵,原田信広,石原悟
  • Organizer: 第34回日本分子生物学会年会.
  • Place of Presentation: 横浜.
• [Presentation] Condensed Chromatin Marked by K27-Trimethy-lated Histone H3 Determines Alterna-tive Promoter Usage of the CYP19 Gene (2011)
  • Author(s): 石原悟,琴村直恵,原田信広
  • Organizer: 第5回日本エピジェネティクス研究会年会
  • Place of Presentation: 熊本
• [Presentation] Condensed chromatin marked by K27-trimethylated histone H3 determinesalternative promoter usage of the CYP19 gene (2011)
  • Author(s): Kotomura,N.,Harada,N.,Ishihara,S
  • Organizer: CDB Symposium"Epigene- tic landscape in development and dis-ease
  • Place of Presentation: Kobe,JAPAN
• [Presentation] アロマターゼ遺伝子CYP19のプロモーター選択に関するエピジェネティクス解析 (2010)
  • Author(s): 石原悟
  • Organizer: 第18回日本ステロイドホルモン学会
  • Place of Presentation: WINC AICHI(愛知県名古屋市)
  • Year and Date: 2010-11-27
• [Presentation] Sequentialrearrangement and eviction of nucleo-somes allow interleukin-2 transcrip-tion following T cell activation. (2010)
  • Author(s): Ishihara,S.,Schwartz,RH
  • Organizer: TheNIBB Conference " DYNAMICGENOME "
  • Place of Presentation: Okazaki, JAPAN
  • Year and Date: 2010-10-16