| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
To examine the chromatin structure in centromere region, we tried to identify proteins that associate with the CENP-T/CENP-W complex based on high sensitive Mass Spectrometry combined with the immunoprecipitation. We identified a protein Eaf6 and we confirmed that this localized to the centromeres. We also identified a histone acethyltransferase as an Eaf6-interacting protein. To examine function of Eaf6, we established an Eaf6-conditional knockout cell line and found that localization of several centromere proteins were abolished in Eaf6-deficient cells based on SILAC analysis, indicating that the Eaf6 complex might be required for chromatin architecture of centromeres through the acethyltransferase activity of the Eaf6 complex.
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