• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Why are microsatellites unstable?

Research Project

Project/Area Number 22570010
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Genetics/Genome dynamics
Research InstitutionNational Hospital Organization, Kyushu Cancer Center

Principal Investigator

ODA Shinya  独立行政法人国立病院機構(九州がんセンター臨床研究センター), その他の部局等 (40333372)

Co-Investigator(Renkei-kenkyūsha) KURAOKA Isao  大阪大学, 大学院・基礎工学研究科, 准教授 (60335396)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsゲノム構築・再編・維持 / ゲノム不安定性 / 癌 / DNA 修復 / ゲノム / DNA修復
Research Abstract

This study aimed to understand the molecular mechanisms that underlie the diversity of microsatellites in human populations and the instability of those in the pathological conditions, particularly, in tumour cells, and, consequently, to elucidate the bona fide dynamics of microsatellites and its pathogenic significance in higher eukaryotic organisms. First, we observed the extreme paucity of polymorphisms in mononucleotide microsatellites among human populations, which makes a remarkable contrast to the dinucleotide microsatellite dynamics. We next analysed mononucleotide and dinucleotide microsatellite alterations under various genetic backgrounds and, consequently, found that the dynamics of these two categories of microsatellites are distinctive depending on the defects in DNA mismatch repair (MMR) and DNA polymerase proofreading (PPR). Analyses using mouse embryo fibroblasts lacking both MMR and PPR are now underway.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (13 results)

All 2013 2012 2011 2010 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (9 results) Remarks (1 results)

  • [Journal Article] Efficient long DNA gap-filling in a mammalian cell-free system: A potential new in vitro DNA replication assay2013

    • Author(s)
      Nakao S, Zhang S, Vaara M, Syvaoja JE, Lee MY, Tsurimoto T, Karran P, Oda S
    • Journal Title

      Biochimie

      Volume: 95 Issue: 2 Pages: 320-328

    • DOI

      10.1016/j.biochi.2012.09.031

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Concurrent genetic alterations in DNA polymerase proofreading and mismatch repair in human colorectal cancer.2011

    • Author(s)
      Yoshida R, Miyashita K, Inoue M, Shimamoto A, Zhao Y, Egashira A, Oki E, Kakeji Y, Oda S, Maehara Y
    • Journal Title

      Eur J Hum Genet

      Volume: 19 Issue: 3 Pages: 320-325

    • DOI

      10.1038/ejhg.2010.216

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Concurrent genetic alterations in DNA polymerase proofreading and mis match repair in human colorectal cancer.2011

    • Author(s)
      Yoshida R, et al.
    • Journal Title

      Eur J Hum Genet

      Volume: 19 Pages: 320-325

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 細胞粗抽出液中で長い単鎖領域に誘導されるDNA 合成反応の性質とin vitro DNA 複製系としての可能性2012

    • Author(s)
      中尾精希、織田信弥
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-14
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞粗抽出液中で長い単鎖領域に誘導されるDNA合成反応の性質とin vitro DNA複製系としての可能性2012

    • Author(s)
      中尾精希
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] 2 本鎖環状DNA 基質と哺乳動物細胞粗抽出液をもちいたDNA 複製効率の定量化2011

    • Author(s)
      中尾精希、織田信弥
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-15
    • Related Report
      2012 Final Research Report
  • [Presentation] 2本鎖環状DNA基質と哺乳動物細胞粗抽出液を用いたDNA複製効率の定量化2011

    • Author(s)
      中尾精希、織田信弥
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜市
    • Year and Date
      2011-12-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒトがんにみられるDNA ポリメラーゼ校正機変異2011

    • Author(s)
      織田信弥
    • Organizer
      日本人類遺伝学会第56回大会
    • Place of Presentation
      千葉
    • Year and Date
      2011-11-11
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒトがんにみられるDNAポリメラーゼ校正機能変異2011

    • Author(s)
      織田信弥
    • Organizer
      日本人類遺伝学会第56回大
    • Place of Presentation
      千葉市
    • Year and Date
      2011-11-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒト大腸癌におけるDNA ポリメラーゼ校正機能およびミスマッチ修復の遺伝学的並存変化2011

    • Author(s)
      吉田倫太郎、宮下要、井上真由子、島本章義、趙岩、掛地吉弘、織田信弥、前原喜彦
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2011-10-05
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト大腸癌におけるDNAポリメラーゼ校正機能およびミスマッチ修復の遺伝学的並存変化2011

    • Author(s)
      吉田倫太郎, ら
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋市
    • Year and Date
      2011-10-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] 定量的遺伝子発現調節と形質変化・薬剤感受性バイオマーカーを例に2010

    • Author(s)
      織田信弥、若狭健太郎
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.ia-nkcc.jp/rinsho/

    • Related Report
      2012 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi