Evolutionary and Physiological Studies of Plant Stress Tolerance using the Liverwort Marchantia polymorpha.

• Project/Area Number: 22570035
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Saitama University
• Principal Investigator: TAKEZAWA Daisuke 埼玉大学, 大学院・理工学研究科, 准教授 (20281834)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 植物 / コケ植物 / ストレス / アブシジン酸 / 受容体 / ゼニゴケ / 苔類 / 適応 / 進化 / ストレス耐性 / プロモーター / PP2C

Research Abstract

Abscisic acid (ABA) is known to be a hormone involved in seed dormancy and germination, as well as stomata closure in flowering plants but its role in lower land plants is not clearly understood. In this study, molecular mechanisms of ABA responses in liverworts were analyzed by using Marchatia polymorpha, and identity of ABA-responsive genes, promoter sequence as well as structures of ABA receptors have been calrified.

Research Products

• [Journal Article] Cold acclimation in the moss Physcomitrella patens involves abscisicacid-dependent signaling (2012)
  • Author(s): Bhyan SB, Minami A, Kaneko Y, Suzuki S, Arakawa K, Sakata Y, Takezawa D
  • Journal Title: Journal of Plant Physiology Volume: 169 Pages: 137-145
• [Journal Article] Cold acclimation in the moss Physcomitrella patens involves abscisic acid-dependent signaling (2012)
  • Author(s): Salma Begum Bhyan
  • Journal Title: Journal of Plant Physiology Volume: 169 Pages: 137-145
  • Peer Reviewed
• [Journal Article] Abscisic acid in bryophytes: how auniversal growth regulator in life becamea plant hormone? (2011)
  • Author(s): Takezawa D, Komatsu K, Sakata Y
  • Journal Title: Journal of Plant Research Volume: 124 Pages: 437-453
• [Journal Article] ABA in bryophytes : how a growth regulator in life became a plant hormone? (2011)
  • Author(s): Daisuke Takezawa
  • Journal Title: Journal of Plant Research Volume: 147 Pages: 437-453
  • Peer Reviewed
• [Journal Article] Freezing and dehydration tolerance and the role of stress hormone in bryophytes (2011)
  • Author(s): Daisuke Takezawa
  • Journal Title: Cryobiology and Cryotechnology Volume: 57 Pages: 13-18
  • Peer Reviewed
• [Journal Article] Evolutionarily conserved regulatory mechanisms of abscisic acid signaling in land plants: characterization of ABSCISIC ACID INSENSITIVE1-like type-2C protein phosphatase in the liverwort Marchantia polymorpha (2010)
  • Author(s): Tougane K, Komatsu K, Begum S B, Sakata Y, Ishizaki K, Yamato T, Kohchi T, Takezawa D
  • Journal Title: Plant Physiology Volume: 152 Pages: 1529-1543
• [Journal Article] Evolutionarily conserved regulatory mechanisms of abscisic acid signaling in land plants : characterization of ABSCISIC ACID INSENSITIVE1-like type-2C protein phosphatase in the liverwort Marchantia polymorpha (2010)
  • Author(s): Ken Tougane
  • Journal Title: Plant Physiology Volume: 152 Pages: 1529-1543
  • Peer Reviewed
• [Journal Article] Endoplasmic reticulum-localized small heat shock protein that accumulates in mulberry tree (Morus bombycis Koidz.) during seasonal cold acclimation is responsive to abscisic acid. (2010)
  • Author(s): Norifumi Ukaji
  • Journal Title: Tree Phyisiology Volume: 30 Pages: 501-513
  • Peer Reviewed
• [Presentation] ゼニゴケ無性芽のアブシジン酸応答とストレス耐性 (2012)
  • Author(s): 竹澤大輔
  • Organizer: 日本植物生理学会
  • Place of Presentation: 京都(京都産業大学)
  • Year and Date: 2012-03-16
• [Presentation] Morphological changes in chloroplasts induced by abscisic acid in Marchantia polymorpha (2012)
  • Author(s): Sato, Y., Kato, M., Akter, K., Kaneko, Y. and Takezawa ,D
  • Organizer: Marchantia Workshop
  • Place of Presentation: Japan
• [Presentation] Abscisic acid receptors in liverworts (2012)
  • Author(s): Murai, S., Yasuda, S. and Takezawa ,D
  • Organizer: Marchantia Workshop
  • Place of Presentation: Japan
• [Presentation] Functional analysis of the SNF1-. ‐ related protein kinase2 to environmental stress in Physcomitrella patens (2012)
  • Author(s): Arai, K. and Takezawa ,D
  • Organizer: Marchantia Workshop
  • Place of Presentation: Japan
• [Presentation] Characterization of a Marchantia mutant with abnormal sensitivity to abscisic acid Marchantia Workshop (2012)
  • Author(s): Kato, M., Sato, Y. and Takezawa ,D
  • Place of Presentation: Japan
• [Presentation] Abscisic acid-induced desiccation stress tolerance in gemmae of Marchantia polymorpha (2012)
  • Author(s): Takezawa ,D., Akter, K., Kaneko, Y., Murai ,S., Kato, M., Sato, Y., Komatsu ,K and Sakata ,Y
  • Organizer: Marchantia Workshop
  • Place of Presentation: Japan
• [Presentation] ゼニゴケ無性芽のアブシジン酸応答とストレス耐性 (2012)
  • Author(s): 竹澤大輔
  • Organizer: 第53回日本植物生理学会年会シンポジウム
  • Place of Presentation: 京都
• [Presentation] 陸上植物の低温応答におけるカルシウムの『普遍的』役割とは? (2012)
  • Author(s): 竹澤大輔
  • Organizer: 日本植物学会第76回大会シンポジウム「植物の低温応答におけるカルシウムの役割」
  • Place of Presentation: 姫路
• [Presentation] ヒメツリガネゴケアブシジン酸合成欠損株の低温馴化および高浸透圧応答 (2012)
  • Author(s): 竹澤大輔、鈴木淳史、渡邉直紀、染宮信之介、小林正智、坂田洋一
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 姫路
• [Presentation] ヒメツリガネゴケ SNF1-related protein kinase 2 のアブシジン酸およびストレス応答 における機能 (2012)
  • Author(s): 新井健太、竹澤大輔
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 姫路
• [Presentation] Abscisic acid-induced desiccation stress tolerance in gemmae of Marchantia polymorpha. (2012)
  • Author(s): Takezawa, D.
  • Organizer: Marchantia Workshop 2012
  • Place of Presentation: Kumamoto
  • Invited
• [Presentation] 陸上植物の低温応答におけるカルシウムの『普遍的』役割とは？ (2012)
  • Author(s): 竹澤大輔
  • Organizer: 日本植物学会第76回大会シンポジウム
  • Place of Presentation: 姫路
  • Invited
• [Presentation] コケ植物の生理学的解析から探る陸上植物共通のABA応答機構 (2011)
  • Author(s): 竹澤大輔
  • Organizer: 日本植物学会
  • Place of Presentation: 東京(東京大学)
  • Year and Date: 2011-09-17
• [Presentation] Abscisic acid (ABA)-induced sugar accumulation and stress tolerance of liverwort Marchantia polymorpha (2011)
  • Author(s): Akter, K., Tougane, K., Kaneko, M. andTakezawa ,D
  • Organizer: International Symposium "Strategies of Plants against Global Environmental Change"
  • Place of Presentation: Japan
• [Presentation] Abscisic acid-induced expression of stress-associated transcripts in the liverwort Marchantia polymorpha.International Symposium (2011)
  • Author(s): Kaneko, M., Komatsu, K., Khaleda, A., Sakata, Y., Ishizaki, K., Yamato, K. T., Kohchi, T. and Takezawa ,D
  • Organizer: Strategies of Plants against Global Environmental Change
  • Place of Presentation: Japan
• [Presentation] コケ植物の生理学的解析から探る陸上植物共通のABA応答機構 日本植物学会第75回大会シンポジウム (2011)
  • Author(s): 竹澤大輔,小松憲治,坂田洋一
  • Organizer: Opening a New Era of ABA Research
  • Place of Presentation: 東京
• [Presentation] コケ植物の凍結・脱水耐性とストレスホルモンの役割 (2010)
  • Author(s): 竹澤大輔
  • Organizer: 低温生物工学会第55回セミナ-
  • Place of Presentation: 東京
  • Year and Date: 2010-06-25
• [Presentation] 低温生物工学会 (2010)
  • Author(s): 竹澤大輔
  • Organizer: コケ植物の凍結・脱水耐性とストレスホルモンの役割
  • Place of Presentation: 東京工業大学
  • Year and Date: 2010-06-25
• [Presentation] Abscisic acid ignaling in Marchantiophyta (2010)
  • Author(s): Takezawa ,D
  • Organizer: Marchantia Workshop
  • Place of Presentation: Japan
  • Year and Date: 2010-03-11
• [Presentation] PP2C-mediated abscisic acid signaling in liverworts (2010)
  • Author(s): Salma, B., Komatsu, K., Kaneko, M., Akter, K., Sakata, Y., Ishizaki, K., Yamato, K. T., Kohchi, T. and Takezawa ,D
  • Organizer: MOSS
  • Place of Presentation: Japan,Japan
• [Presentation] Effect of abscisic acid on sugar accumulation and freezing tolerance in theliverwort Marchantia polymorpha (2010)
  • Author(s): Akter, K., Kaneko, M., Tougane, K. and Takezawa ,D
  • Organizer: MOSS
  • Place of Presentation: Japan,Japan
• [Presentation] Abscisic acid-responsive gene expression in liverwort Marchantia polymorpha (2010)
  • Author(s): Kaneko, M., Komatsu, K., Akter, K., Sakata, Y., Ishizaki, K., Yamato, K. T., Kohchi, T. and Takezawa ,D
  • Organizer: MOSS
  • Place of Presentation: Japan
• [Presentation] Identification and characterization of a newly synthesized protein in suspension-cultured cells of the liverwort Marchantia polymorpha during acquisition of desiccation tolerance (2010)
  • Author(s): Hatanaka, R., Furuki, T., Shimizu, T., Kikawada, T., Takezawa ,D.. and Sugawara, Y
  • Organizer: CRYO2010
  • Place of Presentation: United Kingdom
• [Presentation] 竹澤大輔苔類ゼニゴケにおけるアブシジン酸応答性遺伝子発現の解析 (2010)
  • Author(s): 金子緑、小松憲治、Khaleda Akter、坂田洋一、石崎公庸、大和勝幸、河内孝之
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 名古屋
• [Presentation] カルシウムがヒメツリガネゴケ原糸体細胞のストレス応答に与える影響 (2010)
  • Author(s): 矢羽田正人、竹澤大輔
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 名古屋
• [Presentation] 苔類の休眠とストレス応答におけるアブシジン酸の役割 (2010)
  • Author(s): 竹澤大輔
  • Organizer: 日本植物学会第74回大会シンポジウム
  • Place of Presentation: 名古屋