| Project/Area Number |
22570037
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Kyoto Sangyo University (2011-2012)
Tokyo Institute of Technology (2010) |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | オルガネラ / 葉緑体 / 酸化還元制御 / 植物 / タンパク質 / ジスルフィド結合 / 酸化還元調節 / ストロマ / 蛋白質 |
|---|
| Research Abstract |
The redox state of higher plant chloroplasts fluctuates widely under light and dark conditions. Depending on the redox state, thioredoxins (Trx) regulate the function of proteins by reducing their disulfide bridge. In Arabidopsis, plastidial stroma Trx-isoforms comprise ten members, into five types. The f- and m-type Trxs regulate the activity of enzymes mainly involved in the primary metabolism, including the Calvin cycles, while x-, y- and z-type Trxs are related with oxidative stress responses. However, the genetic evidence for its physiological importance is lacking. We prepared specific antibodies for each Trx isoforms and determined their concentration. The m-type Trx with four isoforms was major Trx proteins in the chloroplast stroma. We analyzed an Arabidopsis trx m mutant.
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