Regulatory mechanism of SAP130 mRNA processing factor in plant morphogenesis

• Project/Area Number: 22570041
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: TSUGE Tomohiko 京都大学, 化学研究所, 助教 (50291076)
• Research Collaborator: AKI Shiori 京都大学, 化学研究所, 研究員 ; NAKAI Hideto 京都大学, 理学研究科, 大学院生 ; GOTO Kakeru 京都大学, 理学研究科, 大学院生 ; DOHMAE Naoshi 理化学研究所, バイオ解析チーム, チームリーダー ; MIYATAKE Hideyuki 理化学研究所, バイオ解析チーム, 研究員
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: RNA代謝制御 / タンパク質分解制御 / 植物形態形成 / 花粉形成 / スプライソソーム / COP9シグナロソーム / 遺伝子発現 / 転写 / SAP130 / SF3b / 花粉形態形成 / mRNA代謝制御 / タンパク質相互作用

Research Abstract

We aim to reveal the molecular function of SAP130 in plant morphogenesis. In Arabidopsis, the promoter activity of SAP130 was detected at a specific stage of pollen development. Coincidently, pollen from AtSAP130 RNAi plants showed low viability and developmental defects at this stage. We then analyzed plants partially complementing csn1 mutant with CSN1, to find that they also exhibited pollen developmental defect in similar manners. Here we hypothesize that CSN and SAP130 coordinately regulate mechanisms required for pollen development.

Research Products

• [Journal Article] The Arabidopsis COP9 SIGNALOSOME INTERACTING F-BOX KELCH 1 protein forms an SCF ubiquitin ligase and regulates hypocotyl elongation (2013)
  • Author(s): Franciosini A, Lombardi B, Iafrate S, Pecce V, Mele G, Lupacchini L, Kondou Y, Gusmaroli G, Aki S, Tsuge T, Deng XW, Matsui M, Vittorioso P, Costantino P, Serino G
  • Journal Title: Mol Plant Volume: Mar 9. Epub ahead of print Issue: 5 Pages: 1616-1629
  • DOI: 10.1093/mp/sst045
  • Peer Reviewed
• [Journal Article] The minimal deneddylase core of the COP9 signalosome excludes the Csn6 MPN -domain (2012)
  • Author(s): Pick E, Golan A, Zimbler JZ, Guo L, Sharabi Y, Tsuge T, Wei N
  • Journal Title: PLoS ONE Volume: 7 Issue: 8 Pages: e43980-e43980
  • DOI: 10.1371/journal.pone.0043980
  • Peer Reviewed
• [Journal Article] AtSap130/AtSF3b-3 function is required for reproduction in Arabidopsis thaliana (2011)
  • Author(s): Aki, S., Nakai, H., Aoyama, T., Oka, A., and Tsuge, T
  • Journal Title: Plant Cell Physiol Volume: 52 Issue: 8 Pages: 1330-1339
  • DOI: 10.1093/pcp/pcr077
  • NAID: 10029713258
  • Peer Reviewed
• [Journal Article] CSN1 inhibits c-Jun phosphorylation and down-regulates ectopic expression of JNK1 (2011)
  • Author(s): Tsuge T, Menon S, Tong Y, Wei N.
  • Journal Title: Protein Cell Volume: 2 Issue: 5 Pages: 423-432
  • DOI: 10.1007/s13238-011-1043-0
  • Peer Reviewed
• [Journal Article] AtSap130/AtSF3b-3 function is required for reproduction Arabidopsis thaliana (2011)
  • Author(s): Aki, S., et al
  • Journal Title: Plant & Cell Physiology Volume: 52 Issue: 4 Pages: 1330-1339
  • DOI: 10.1105/tpc.111.083337
  • Peer Reviewed
• [Journal Article] D-myo-inositol-3-phosphate plays an essential role in auxin-regulated embryogenesis by affecting phosphatidylinositol-mediated endomembrane function in Arabidopsis. (2011)
  • Author(s): Luo Y, Qin G, Zhang J, Liang Y, Song Y, Zhao M, Tsuge T, Aoyama T, Liu J, Gu H, Qu LJ.
  • Journal Title: Plant Cell Volume: (印刷中(掲載確定))
  • Peer Reviewed
• [Journal Article] A novel factor FLOURY ENDOSPERM2 is involved in regulation of rice grain size and starch quality. (2011)
  • Author(s): She KC, Kusano H, Koizumi K, Yamakawa H, Hakata M, Imamura T, Fukuda M, Naito N, Tsurumaki Y, Yaeshima M, Tsuge T, Matsumoto K, Kudoh M, Itoh E, Kikuchi S, Kishimoto N, Yazaki J, Ando T, Yano M, Aoyama T, Sasaki T, Satoh H, Shimada H.
  • Journal Title: Plant Cell Volume: 22 Pages: 3280-3294
  • Peer Reviewed
• [Journal Article] A novel factor FLOURY ENDOSPERM2 is involved in regulation of rice grain size and starch quality (2010)
  • Author(s): She KC, Kusano H, Koizumi K, Yamakawa H, Hakata M, Imamura T, Fukuda M, Naito N, Tsurumaki Y, Yaeshima M, Tsuge T, Matsumoto K, Kudoh M, Itoh E, Kikuchi S, Kishimoto N, Yazaki J, Ando T, Yano M, Aoyama T, Sasaki T, Satoh H, Shimada H
  • Journal Title: Plant Cell Volume: 22 Issue: 10 Pages: 3280-3294
  • DOI: 10.1105/tpc.109.070821
  • Peer Reviewed
• [Journal Article] Comparative analysis of local green tea in Korea by STS-RFLP (2010)
  • Author(s): Cho KH, Jo A, Tsuge T, Kim JC, Kim R, Yoon HS, Kim GT
  • Journal Title: J Life Sci Volume: 20 Issue: 9 Pages: 1415-1419
  • DOI: 10.5352/jls.2010.20.9.1415
  • Peer Reviewed
• [Journal Article] Comparative genomic analysis of Korean and Japanese green tea trees by using molecular markers. (2010)
  • Author(s): Cho KH, Lee EJ, Tsuge T, Jo A, Kim JC, Cheong GW, Yoon HS, Kim GT
  • Journal Title: Can J Plant Sci Volume: 90 Pages: 293-298
  • URL: http://pubs.aic.ca/doi/abs/10.4141/CJPS09065
  • Peer Reviewed
• [Journal Article] Comparative analysis of local green tea in Korea by STS-RFLP. (2010)
  • Author(s): Cho KH, Jo A, Tsuge T, Kim JC, Kim R, Yoon HS, Kim GT.
  • Journal Title: J Life Sci Volume: 20 Pages: 1415-1419
  • Peer Reviewed
• [Presentation] COP9シグナロソームとスプライソソーム構成因子群は植物形態形成に重要な役割を担う (2013)
  • Author(s): 安喜史織、張俊、後藤翔、中井秀人、岡穆宏、青山卓史、瞿礼嘉、*柘植知彦
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学、岡山県岡山市
• [Presentation] COP9シグナロソームと結合するPrp43 RNAヘリカーゼの機能解析 (2013)
  • Author(s): *後藤翔、青山卓史、柘植知彦
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学、岡山県岡山市
• [Presentation] COP9シグナロソームと相互作用するTrihelix proteinの機能解析 (2013)
  • Author(s): *中井秀人、安喜史織、HEYL Alexander、青山卓史、柘植知彦
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学、岡山県岡山市
• [Presentation] Understanding CSN through CSN1 and its binding partners (2012)
  • Author(s): *Tsuge T
  • Organizer: Invited lecture at "Symposium on Plant Biology and Agrobiotechnology"
  • Place of Presentation: Peking University, Beijing PRC.
  • Year and Date: 2012-10-17
• [Presentation] COP9 signalosome and its interacting protein SAP130 are important for pollen development in Arabidopsis (2012)
  • Author(s): Aki S, Nakai H, Goto K, Oka A, Aoyama T, *Tsuge T
  • Organizer: ZOMES-VII "Ubiquitin family proteins and their cognate PCI complexes"
  • Place of Presentation: Munich GERMANY
• [Presentation] Characterization of a spliceosomal RNA helicase interacting with CSN1 in Arabidopsis (2012)
  • Author(s): *Goto K, Aoyama T, Tsuge T
  • Organizer: ZOMES-VII "Ubiquitin family proteins and their cognate PCI complexes"
  • Place of Presentation: Munich GERMANY.
• [Presentation] Involvement of phospholipid signaling in root hair development (2012)
  • Author(s): Anzai N, Ohashi Y, Tsuge T, *Aoyama T
  • Organizer: Plant development and environmental interactions - 3rdEMBO conference on plant molecular biology
  • Place of Presentation: Matera ITALY
• [Presentation] COP9 signalosome, the master regulator of photomorphogenesis, and its interacting protein SAP130 are important for pollen development inArabidopsis (2012)
  • Author(s): Aki S, Nakai H, Aoyama T, Oka A, *Tsuge T
  • Organizer: The 1st International Symposium on Plant Environmental Sensing
  • Place of Presentation: Todaiji Culture Center, Nara NARA
• [Presentation] Functional analysis of a trihelix protein interacting with COP9 signalosome. (2012)
  • Author(s): Nakai H, Aki S, Heyl A, Aoyama T, Tsuge T
  • Organizer: The 1st International Symposium on Plant Environmental Sensing
  • Place of Presentation: Todaiji Culture Center, Nara NARA.
• [Presentation] COP9シグナロソームとその結合因子のSAP130は花粉形成において重要な役割を担う (2012)
  • Author(s): 安喜史織、中井秀人、岡穆宏、青山卓史、*柘植知彦
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学、京都府京都市
• [Presentation] COP9シグナロソームと相互作用するTrihelix proteinの機能解析 (2012)
  • Author(s): 中井秀人、安喜史織、HEYL Alexander、青山卓史、柘植知彦
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学、京都府京都市
• [Presentation] リン酸欠乏に応答した根毛伸長におけるPIP5Kの機能 (2012)
  • Author(s): 和田悠貴香、安田敬子、柘植知彦、青山卓史
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学、京都府京都市
• [Presentation] COP9 signalosome and its interacting protein SAP130 are important for pollen development in Arabidopsis. (2012)
  • Author(s): Aki S, Nakai H, Goto K, Oka A, Aoyama T, Tsuge T.
  • Organizer: ZOMES-VII “Ubiquitin family proteins and their cognate PCI complexes”
  • Place of Presentation: Munich GERMANY.
• [Presentation] Characterization of a spliceosomal RNA helicase interacting with CSN1 in Arabidopsis. (2012)
  • Author(s): Goto K, Aoyama T, Tsuge T.
  • Organizer: ZOMES-VII “Ubiquitin family proteins and their cognate PCI complexes”
  • Place of Presentation: Munich GERMANY.
• [Presentation] Involvement of phospholipid signaling in root hair development. (2012)
  • Author(s): Anzai N, Ohashi Y, Tsuge T, Aoyama T.
  • Organizer: Plant development and environmental interactions - 3rd EMBO conference on plant molecular biology
  • Place of Presentation: Matera ITALY.
• [Presentation] COP9 signalosome, the master regulator of photomorphogenesis, and its interacting protein SAP130 are important for pollen development in (2012)
  • Author(s): Aki S, Nakai H, Aoyama T, Oka A, Tsuge T
  • Organizer: The 1st International Symposium on Plant Environmental Sensing
  • Place of Presentation: Todaiji Culture Center, Nara NARA
• [Presentation] Functional analysis of a trihelix protein interacting with COP9 signalosome (2012)
  • Author(s): Nakai H, Aki S, Heyl A, Aoyama T, Tsuge T
  • Organizer: The 1st International Symposium on Plant Environmental Sensing
  • Place of Presentation: Todaiji Culture Center, Nara NARA
• [Presentation] AtSAP130/AtSF3b-3 function is required for pollen development in Arabidopsis thaliana (2011)
  • Author(s): Aki S, Nakai H, Aoyama T, Oka A, Tsuge T
  • Organizer: International Plant RNA Workshop 2011
  • Place of Presentation: RIKEN Yokohama Institute, Yokohama KANAGAWA.
• [Presentation] COP9シグナロソーム結合因子SAP130はシロイヌナズナの花粉形成において重要な役割を担う (2011)
  • Author(s): 安喜史織、中井秀人、岡穆宏、青山卓史、柘植知彦
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学川内北キャンパス、宮城県仙台市
• [Presentation] COP9シグナロソームと相互作用するTrihelix proteinの機能解析 (2011)
  • Author(s): 中井秀人、安喜史織、HEYL Alexander、青山卓史、柘植知彦
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学川内北キャンパス、宮城県仙台市
• [Presentation] シロイヌナズナ・ホスホリパーゼDz1遺伝子の遺伝学的解析 (2011)
  • Author(s): 安齋尚子、大橋洋平、谷口雅俊、柘植知彦、青山卓史
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学川内北キャンパス、宮城県仙台市
• [Presentation] AtSAP130/AtSF3b-3 function is required for pollen development in Arabidopsis thaliana (2011)
  • Author(s): Aki S, Nakai H, Aoyama T, Oka A, Tsuge T
  • Organizer: International Plant RNA Workshop 2011
  • Place of Presentation: RJKEN Yokohama Institute, Yokohama KANAGAWA
• [Presentation] シロイヌナズナ・ホスホリパーゼDζ1遺伝子の遺伝学的解析 (2011)
  • Author(s): 安齋尚子、大橋洋平、谷口雅俊、柘植知彦、青山卓史
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学川内北キャンパス、宮城県仙台市
• [Presentation] 環境刺激に応答したPIK5K遺伝子の機能 (2010)
  • Author(s): 和田悠貴香、草野博彰、安田敬子、柘植知彦、青山卓史
  • Organizer: 第23回植物脂質シンポジウム
  • Place of Presentation: 京都大学宇治キャンパスおうばくプラザ、京都府宇治市
• [Presentation] シロイヌナズナ・ホスホリパーゼDz1遺伝子の遺伝学的解析 (2010)
  • Author(s): 安齋尚子、大橋洋平、谷口雅俊、柘植知彦、青山卓史
  • Organizer: 第23回植物脂質シンポジウム
  • Place of Presentation: 京都大学宇治キャンパスおうばくプラザ、京都府宇治市
• [Presentation] CSN1 interacting protein SAP130 is important for pollen development in Arabidopsis (2010)
  • Author(s): Aki S, Nakai H, Oka A, Tsuge T
  • Organizer: ZOMES-VI (The Sixth International Symposium on the COP9 signalosome, Proteasome and eIF3: Expanding the PCI family beyond proteasome CSN and cIF3 complexes)
  • Place of Presentation: Safed ISRAEL
• [Presentation] Arabidopsis F-box proteins PIC2a and b form SCF ubiquitin ligases which interact with the COP9 signalosome and regulate light response (2010)
  • Author(s): Serino G, Lombardi B, Iafrate S, Franciosini A, Mele G, Heyl A, Tsuge T, Aki S, Kondou Y, Matsui M, Costantino P
  • Organizer: ZOMES-VI (The Sixth International Symposium on the COP9 signalosome, Proteasome and eIF3: Expanding the PCI family beyond proteasome CSN and cIF3 complexes)
  • Place of Presentation: Safed ISRAEL
• [Presentation] Functions of PIP5K genes in the root hair elongation responsive to environmental stimuli. (2010)
  • Author(s): Wada Y, Kusano H, Yasuda K, Tsuge T, Aoyama T
  • Organizer: 19th International Symposium on Plant Lipids
  • Place of Presentation: Cairns AUSTRALIA
• [Presentation] The Arabidopsis phosphatidylinositol phosphate 5-kinase PIPK3 At2g26420 is a key regulator of root hair tip growth (2010)
  • Author(s): Kusano H, Testerink C, Vermeer JEM, Tsuge T, Shimada H, Oka A, Munnik T, Aoyama T
  • Organizer: The 21st International Conference on Arabidopsis Research
  • Place of Presentation: Pacifico Yokohama, Yokohama KANAGAWA
• [Presentation] Involvement of Arabidopsis thaliana phospholipase Dz2 in root hydropism through the suppression of root gravitropism (2010)
  • Author(s): Taniguchi YY, Taniguchi M, Tsuge T, Oka A, *Aoyama T
  • Organizer: The 21st International Conference on Arabidopsis Research
  • Place of Presentation: Pacifico Yokohama, Yokohama KANAGAWA
• [Presentation] シロイヌナズナ・ホスホリパーゼDζ1遺伝子の遺伝学的解析 (2010)
  • Author(s): 安齋尚子、大橋洋平、谷口雅俊、柘植知彦、青山卓史
  • Organizer: 第23回植物脂質シンポジウム
  • Place of Presentation: 京都大学宇治キャンパスおうばくプラザ、京都府宇治市
• [Presentation] CSN1 interacting protein SAP130 is important for pollen development in Arabidopsis. (2010)
  • Author(s): Aki S, Nakai H, Oka A, Tsuge T
  • Organizer: ZOMES-VI (The Sixth International Symposium on the COP9 signalosome, Proteasome and eIF3 : Expanding the PCI family beyond proteasome CSN and cIF3 complexes)
  • Place of Presentation: Safed ISRAEL
• [Presentation] Arabidopsis F-box proteins PIC2a and b form SCF ubiquitin ligases which interact with the COP9 signalosome and regulate light response. (2010)
  • Author(s): Giovanna S, Lombardi B, Iafrate S, Franciosini A, Mele G, Heyl A, Tsuge T, Aki S, Kondou Y, Matsui M, Costantino P
  • Organizer: ZOMES-VI (The Sixth International Symposium on the COP9 signalosome, Proteasome and eIF3 : Expanding the PCI family beyond proteasome CSN and cIF3 complexes)
  • Place of Presentation: Safed ISRAEL
• [Presentation] The Arabidopsis phosphatidylinositol phosphate 5-kinase PIPK3 At2g26420 is a key regulator of root hair tip growth. (2010)
  • Author(s): Kusano H, Testerink C, Vermeer JE, Tsuge T, Shimada H, Oka A, Munnik T, Aoyama T
  • Organizer: The 21st International Conference on Arabidopsis Research
  • Place of Presentation: Pacifico Yokohama, Yokohama KANAGAWA
• [Presentation] Involvement of Arabidopsis thaliana phospholipase Dζ2 in root hydropism through the suppression of root gravitropism. (2010)
  • Author(s): Taniguchi YY, Taniguchi M, Tsuge T, Oka A, Aoyama T
  • Organizer: The 21st International Conference on Arabidopsis Research
  • Place of Presentation: Pacifico Yokohama, Yokohama KANAGAWA
• [Presentation] Understanding CSN through CSN1 and its binding partners.
  • Author(s): Tsuge T.
  • Organizer: Symposium on Plant Biology and Agrobiotechnology
  • Place of Presentation: Peking University, Beijing PRC.
  • Invited
• [Book] 最新 植物生理化学 (2011)
  • Author(s): 長谷川宏司・広瀬克利
  • Publisher: 大学教育出版 岡山
• [Book] 「最新植物生理化学」(長谷川宏司・広瀬克利)第1章光受容体と光情報伝達 (2011)
  • Author(s): 柘植知彦、安喜史織
  • Publisher: 大学教育出版岡山
• [Book] 『ふしぎ』を科学しよう 命はどのようにして生まれたの? (2010)
  • Author(s): 池内了監修
  • Publisher: かもがわ出版 京都
• [Book] 『ふしぎ』を科学しよう命はどのようにしで生まれたの?(池内了 監修)(葉は緑色なのに、花の色はなぜいろいろあるのですか?秋になると紅葉がおこるのはなぜ?) (2010)
  • Author(s): 柘植知彦
  • Total Pages: 95
  • Publisher: かもがわ出版京都
• [Remarks]
  • URL: http://www.scl.kyoto-u.ac.jp/~molbio/index.html
• [Remarks]
  • URL: http://molbio.kuicr.kyoto-u.ac.jp/mbl/publication.html
• [Remarks]
  • URL: http://rdb.kuicr.kyoto-u.ac.jp/researchers/view/tsuge+tomohiko