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Dual role for plant purine catabolism and its regulation

Research Project

Project/Area Number 22570043
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionHiroshima University

Principal Investigator

SAKAMOTO Atsushi  広島大学, 大学院・理学研究科, 教授 (60270477)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsプリン分解 / 成長生理 / ストレス / シロイヌナズナ / イネ / プリン代謝 / キサンチン脱水素酵素 / 植物 / ウレイド / 窒素転流 / 環境適応 / 窒素リサイクル / 尿酸オキシダーゼ / ストレス適応 / 乾燥 / 尿酸 / 活性酸素
Research Abstract

Plant purine catabolism has a relatively long history of physiological and biochemical studies particularly in ureide-type leguminous species, becauseit constitutes an integral part of the storage and translocation of symbiotically fixed nitrogen. In contrast, for the vast majority of plants that do not utilize a symbiotic association with Rhizobiumbacteria, the general role and physiological significance of purine degradation remains elusive. We found that dysfunction of purine catabolism impaired the plant growth under normal conditions and enhanced the stress sensitivity in hash environment, indicating a dual role in maintaining the growth and contributing to adaptation to stress.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (53 results)

All 2013 2012 2011 2010 Other

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (34 results) Remarks (4 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] X-ray crystal structure of a mutant assimilatory nitrite reductase that shows sulfite reductase-like activity2012

    • Author(s)
      Nakano S, Takahashi M, Sakamoto A, Morikawa H, Katayanagi K
    • Journal Title

      Chemistry & Biodiversity

      Volume: 9 Pages: 1989-1999

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsiscotyledon chloroplast biogenesis factor CYO1 uses glutathione as an electron donor and interacts with PSI (A1 and A2) and PSII (CP43 and CP47) subunits2012

    • Author(s)
      Muranaka A, Watanabe S, Sakamoto A, Shimada H
    • Journal Title

      Journal of Plant Physiology

      Volume: 169 Pages: 1212-1215

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The reductive reaction mechanism of tobacco nitrite reductase derived from a combination of crystal structures and UV-Vis microspectroscopy2012

    • Author(s)
      Nakano S, Takahashi M, Sakamoto A, Morikawa H, Katayanagi K
    • Journal Title

      Proteins

      Volume: 80 Pages: 2035-2045

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differences in intron-mediated enhancement of gene expression by the first intron of cytosolic superoxide dismutase gene from rice in monocot and dicot plants.2012

    • Author(s)
      Morita S, Tsukamoto S, Sakamoto A, Makino H, Nakauji E, Kaminaka H, Masumura T, Ogihara Y, Satoh S, Tanaka K
    • Journal Title

      Plant Biotechnology

      Volume: 29 Pages: 115-119

    • NAID

      10030561667

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structure- function relationship of assimilatory nitrite reductases from the leaf and root of tobacco based on the high resolution structure2012

    • Author(s)
      Nakano S, Takahashi M, Sakamoto A, Morikawa H, Katayanagi K
    • Journal Title

      Protein Science

      Volume: 21 Pages: 383-395

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structure-function relationship of assimilatory nitrite reductases from the leaf and root of tobacco based on high-resolution structures2012

    • Author(s)
      S. Nakano, M. Takahashi, A. Sakamoto, H. Morikawa, K. Katayanagi
    • Journal Title

      Protein Sci

      Volume: 21(3) Issue: 3 Pages: 383-395

    • DOI

      10.1002/pro.2025

    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differences in intron-mediated enhancement of gene expression by the first intron of cytosolic superoxide dismutase gene from rice in monocot and dicot plants2012

    • Author(s)
      Shigeto Morita
    • Journal Title

      Plant Biotechnology

      Volume: 29 Issue: 1 Pages: 115-119

    • DOI

      10.5511/plantbiotechnology.11.1207a

    • NAID

      10030561667

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis cotyledon chloroplast biogenesis factor CYO1 uses glutathione as electron donor and interacts with PSI (A1 and A2) and PSII (CP43 and CP47)subunits2012

    • Author(s)
      Muranaka, A., et al.
    • Journal Title

      Journal of Plant Physiology

      Volume: 169 Issue: 12 Pages: 1212-1215

    • DOI

      10.1016/j.jplph.2012.04.001

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prolonged exposure to atmospheric nitrogen dioxide increases fruit yield of tomato plants2011

    • Author(s)
      Takahashi M, Sakamoto A, Ezura H, Morikawa H
    • Journal Title

      Plant Biotechnology

      Volume: 28 Pages: 485-487

    • NAID

      10030304072

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Normalization using ploidy and genomic DNA copy number allows absolute quantification of transcripts, proteins and metabolites in cells2010

    • Author(s)
      Shimada H, Obayashi T, Takahashi N, Matsui M, Sakamoto A
    • Journal Title

      Plant Methods

      Volume: 6 Pages: 29-29

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] RNA interference- mediated suppression of xanthine dehydrogenase reveals the role of purine metabolism in drought tolerance in Arabidopsis2010

    • Author(s)
      Watanabe S, Nakagawa A, Izumi S, Shimada H, Sakamoto A
    • Journal Title

      FEBS Letters

      Volume: 584 Pages: 1181-1186

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Normalization using ploidy and genomic DNA copy number allows absolute quantification of transcripts, proteins and metabolites in cells2010

    • Author(s)
      H.Shimada, et al.
    • Journal Title

      Plant Methods

      Volume: 6 Pages: 29-29

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RNA interference-mediated suppression of xanthine dehydrogenase reveals the role of purine metabolism in drought tolerance in Arabidopsis.2010

    • Author(s)
      S.Watanabe, et al.
    • Journal Title

      FEBS Letters

      Volume: 584 Pages: 1181-1186

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] ウレイド化合物を蓄積するシロイヌナズナ変異株のストレス生理学的解析2013

    • Author(s)
      高木 紘,渡邊俊介,島田裕士,坂本 敦
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Year and Date
      2013-03-23
    • Related Report
      2012 Final Research Report
  • [Presentation] イネCYO1 (子葉特異的葉緑体形成因子) ホモログの解析2013

    • Author(s)
      中原恭俊, 水谷春香, 村中厚子, 渡邊俊介, 坂本 敦, 島田裕士
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Year and Date
      2013-03-23
    • Related Report
      2012 Final Research Report
  • [Presentation] 大気中二酸化窒素の植物ホルモン様作用による細胞増殖and/or 拡大に関与する遺伝子発現の解析2013

    • Author(s)
      高橋美佐, 坂本 敦, 塚谷裕一, 森川弘道
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Year and Date
      2013-03-23
    • Related Report
      2012 Final Research Report
  • [Presentation] ストレス適応における核酸塩基代謝の作用機序と代謝中間体の役割2013

    • Author(s)
      渡邊俊介,松本真由美,鍛冶村光也,箱守優毅,高木 紘,島田裕士,坂本 敦
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Year and Date
      2013-03-21
    • Related Report
      2012 Final Research Report
  • [Presentation] Theoretical inference of the influence of metabolites on gene expression based on publicly available sets of microarray data.2013

    • Author(s)
      Miyamoto M, Watabnabe S, Sakamoto A, Nishimori H, Awazu A
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Year and Date
      2013-03-21
    • Related Report
      2012 Final Research Report
  • [Presentation] 酸素酸化によって不活化したルビスコのCYO2 による再活性化機構の解析2013

    • Author(s)
      室屋誠人, 伊東千賀子, 村中厚子, 西村浩二, 白上典彦,岡村文音, 渡邊俊介, 坂本 敦, 島田裕士
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Year and Date
      2013-03-21
    • Related Report
      2012 Final Research Report
  • [Presentation] Role of nucleobase metabolism in stress protection of Arabidopsis plants2013

    • Author(s)
      Shunsuke Watanabe
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Related Report
      2012 Annual Research Report
  • [Presentation] Stress-physiological characterization of Arabidopsis mutants which over-accumulate ureide compounds2013

    • Author(s)
      Hiroshi Takagi
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山
    • Related Report
      2012 Annual Research Report
  • [Presentation] Revealing a hidden role of nucleobase metabolism in plant strategies coping with stress2012

    • Author(s)
      Sakamoto A, Watanabe S
    • Organizer
      平成24年度鳥取大学乾燥地研究センター共同研究発表会
    • Place of Presentation
      鳥取
    • Year and Date
      2012-12-02
    • Related Report
      2012 Final Research Report
  • [Presentation] 遺伝子発現解析から検証したアラントインの生理作用について2012

    • Author(s)
      高木 紘,渡邊俊介,島田裕士,坂本 敦
    • Organizer
      広島大学大学院・理学研究科数理分子生命理学専攻第4回公開シンポジウム「数理生命科学の新展開 -階層間で自発的に干渉しあう形・動き・機能-」
    • Place of Presentation
      東広島
    • Year and Date
      2012-09-06
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞1個あたりのmRNA数・タンパク質数・代謝産物数の簡易定量法の開発2012

    • Author(s)
      中原恭俊, 大林 武, 坂本 敦, 島田裕士
    • Organizer
      広島大学大学院・理学研究科数理分子生命理学専攻第4回公開シンポジウム「数理生命科学の新展開-階層間で自発的に干渉しあう形・動き・機能-」
    • Place of Presentation
      東広島
    • Year and Date
      2012-09-06
    • Related Report
      2012 Final Research Report
  • [Presentation] 二酸化窒素の植物バイタリゼー ションの研究 (4) 細胞拡大と核内倍加との間に相関関係がなかった2012

    • Author(s)
      高橋美佐,坂本 敦,塚谷裕一,森川弘道
    • Organizer
      第30回日本植物細胞分子生物学会大会・シンポジウム
    • Place of Presentation
      生駒
    • Year and Date
      2012-08-05
    • Related Report
      2012 Final Research Report
  • [Presentation] Protein disulfide isomerase活性を有するシロイヌナズナ子葉特異的葉緑体形成因子CYO1の酵素学的解析2012

    • Author(s)
      村中厚子,渡邊俊介,坂本 敦,島田裕士
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-18
    • Related Report
      2012 Final Research Report
  • [Presentation] シロイヌナズナ葉緑体形成因子CYO2 の解析2012

    • Author(s)
      室屋誠人, 伊東千賀子, 村中厚子, 水谷春香, 渡邊俊介, 坂本 敦, 島田裕士
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-18
    • Related Report
      2012 Final Research Report
  • [Presentation] ストレス適応代謝としてのプリン分解の生理機能解析2012

    • Author(s)
      渡邊俊介,杉本高文,呂 由,島田裕士,坂本 敦
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-16
    • Related Report
      2012 Final Research Report
  • [Presentation] シロイヌナズナにおいてプリン代謝産物に応答する遺伝子発現の網羅的解析2012

    • Author(s)
      高野須由佳,渡邊俊介,山 敦美,島田裕士,坂本 敦
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-16
    • Related Report
      2012 Final Research Report
  • [Presentation] 大気中二酸化窒素の植物バイタリゼーションによる細胞拡大は核内倍加が原因ではない2012

    • Author(s)
      高橋美佐, 坂本 敦, 塚谷裕一, 森川弘道
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-16
    • Related Report
      2012 Final Research Report
  • [Presentation] Revealing a hidden role of nucleobases metabolism in plant strategies coping with stress2012

    • Author(s)
      Atsushi Sakamoto
    • Organizer
      平成24年度鳥取大学乾燥地研究センター共同研究発表会
    • Place of Presentation
      鳥取
    • Related Report
      2012 Annual Research Report
  • [Presentation] 核酸塩基代謝に隠された植物のストレス適応機構戦略の解明2011

    • Author(s)
      坂本 敦,渡邊俊介
    • Organizer
      平成23年度鳥取大学乾燥地研究センター共同研究発表会
    • Place of Presentation
      鳥取
    • Year and Date
      2011-12-04
    • Related Report
      2012 Final Research Report
  • [Presentation] タバコ由来亜硝酸還元酵素Nii3のX線照射による還元誘起を利用した反応機構解明2011

    • Author(s)
      中野祥吾,高橋美佐,坂本 敦,森川弘道,片柳克夫
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Watanabe S, Sugimoto T, LV Y, Shimada H, Sakamoto A2011

    • Author(s)
      Watanabe S, Sugimoto T, LV Y, Shimada H, Sakamoto A
    • Organizer
      International Symposium "Strategies of Plants against Global Environmental Change"
    • Place of Presentation
      Kurashiki, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Can purine metabolites serve as a potential inducer of gene expression? - A transcriptome analysis of Arabidopsis in response to allantoin2011

    • Author(s)
      Watanabe S, Kounosu Y, Shimada H, Sakamoto A
    • Organizer
      International Symposium "Strategies of Plants against Global Environmental Change"
    • Place of Presentation
      Kurashiki, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] The initial N-O bond cleavage mechanism of nitrite in assimilatory-nitrite-reductase from Nicotiana tabacum: effective utilization of X-ray structures and UV-Vis mcrospectroscopy2011

    • Author(s)
      Nakano S, Takahashi M, Sakamoto A, Morikawa H, Katayanagi K
    • Organizer
      15th International Conference on Biological Inorganic Chemistry
    • Place of Presentation
      Vancouver, Canada
    • Related Report
      2012 Final Research Report
  • [Presentation] シロイヌナズナのストレス適応におけるプリン分解代謝の生理的役割2011

    • Author(s)
      渡邊俊介,杉本高文,前田智美,島田裕士,坂本 敦
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台
    • Related Report
      2012 Final Research Report
  • [Presentation] 緑色組織におけるシロイヌナズナ葉緑体形成因子CYO2の解析2011

    • Author(s)
      室屋誠人,伊東千賀子,村中厚子,水谷春香,坂本 敦,島田裕士
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台
    • Related Report
      2012 Final Research Report
  • [Presentation] ゲノムコピー数と倍数性に基づいたABCD (Analysis Based on Copy number of genomic DNA) 法による細胞1個当たりのmRNA数,タンパク質数,代謝産物数の絶対定量法の開発2011

    • Author(s)
      島田裕士,大林 武,高橋直紀,松井 南,坂本 敦
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台
    • Related Report
      2012 Final Research Report
  • [Presentation] 大気中二酸化窒素の植物バイタリゼーション作用による細胞拡大機構の解析2011

    • Author(s)
      高橋美佐,坂本 敦,塚谷裕一,森川弘道
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台
    • Related Report
      2012 Final Research Report
  • [Presentation] Purine ring catabolism is involved in plant drought protection2011

    • Author(s)
      Sakamoto, A., et al
    • Organizer
      International Symposium "Strategies of Plants against Global Environmental Chang"
    • Place of Presentation
      Kurashiki, Japan
    • Related Report
      2011 Annual Research Report
  • [Presentation] Can purine metabolites serve as a potential inducer of gene expression?-A transcriptome analysis of Arabidopsis in response to allantoin2011

    • Author(s)
      Watanabe, S., et al
    • Organizer
      International Symposium "Strategies of Plants against Global Environmental Change"
    • Place of Presentation
      Kurashiki, Japan
    • Related Report
      2011 Annual Research Report
  • [Presentation] 大気中二酸化窒素の植物バイタリゼーション効果の原因遺伝子2010

    • Author(s)
      高橋美佐,柏原俊一,古橋孝将,坂本 敦,江面 浩,森川弘道
    • Organizer
      第28回日本植物細胞分子生物学会大会・シンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-03
    • Related Report
      2012 Final Research Report
  • [Presentation] Physiological contribution of purine metabolism in drought acclimatization of Arabidopsis2010

    • Author(s)
      S.Watanabe, et al.
    • Organizer
      21^<st> International Conference on Arabidopsis Research
    • Place of Presentation
      横浜市
    • Year and Date
      2010-06-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] バコ由来同化型亜硝酸還元酵素群の構造機能解析による詳細な基質進入経路の解明2010

    • Author(s)
      中野祥吾,高橋美佐,坂本 敦,森川弘道,片柳克夫
    • Organizer
      第33回日本分子生物学会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2012 Final Research Report
  • [Presentation] Physiological contribution of purine metabolism in drought acclimatization of Arabidopsis2010

    • Author(s)
      Watanabe S, Nakagawa A, Shimada H, Sakamoto A
    • Organizer
      21st International Congress on Arabidopsis Research "2010 and Beyond."
    • Place of Presentation
      Yokohama, Japan.
    • Related Report
      2012 Final Research Report
  • [Presentation] ABCD, analysis based on copy number of genomic DNA, method reveals the number of transcripts, proteins and metabolites in a cell of Arabidopsis2010

    • Author(s)
      Shimada H, Obayashi T, Sakamoto A
    • Organizer
      21st International Congress on Arabidopsis Research "2010 and Beyond."
    • Place of Presentation
      Yokohama, Japan
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.mls.sci.hiroshima-u.ac.jp/mpb/index.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 受賞 平成24年度科学研究費補助金審査委員表彰(2012年10月31日)

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.mls.sci.hiroshima-u.ac.jp/mpb/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.mls.sci.hiroshima-u.ac.jp/mpb/index.html

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] イネ形質転換体及びその作成方法2013

    • Inventor(s)
      島田裕士,坂本 敦
    • Industrial Property Rights Holder
      国立大学法人広島大学
    • Industrial Property Number
      2013-048259
    • Filing Date
      2013-03-11
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] イネ形質転換体及びその作成方法2013

    • Inventor(s)
      島田裕士,坂本 敦
    • Industrial Property Rights Holder
      国立大学法人広島大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-048259
    • Filing Date
      2013-03-11
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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