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Analysis of bacterial-type plastidial RNA binding protein required for chloroplast development

Research Project

Project/Area Number 22570045
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionKyushu University

Principal Investigator

KUSUMI Kensuke  九州大学, 理学(系)研究科(研究院), 助教 (00304725)

Project Period (FY) 2010-10-20 – 2013-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords色素体機能 / 光合成 / 転写装置 / 翻訳装置 / RNA結合タンパク質 / rRNA / 葉緑体分化 / 葉緑体 / 転写制御 / イネ / シロイヌナズナ / 器官分化 / 温度感受性 / 色素体 / リボソームRNA / rrnオペロン
Research Abstract

NUS1 protein is plastidial RNA binding protein essential for chloroplast biogenesis under low temperature. NUS1 is structurally similar to the bacterial protein NusB required for rRNA expression. NUS1 protein accumulated abundantly in the pre-emerged immature leaf whereas it disappeared in the mature leaf. NUS1 has a molecular weight of 30 kDa, and the BN-PAGE analyses showed that NUS1 was involved in the ~110kDa protein complex. Mobility gel shift assays indicated that NUS1 protein bound to 5' precursor region of chloroplast 16S rRNA. NUS1-deficient mutant showed severe leaf chlorosis at low temperature. In the chlorotic leaves of the mutant seedling, maturation of chloroplast rRNA was impaired and the chloroplast translation/transcription capacity was severely suppressed, suggesting that NUS1 has important roles in the regulation of chloroplast genetic system during early leaf development in rice.

Report

(4 results)
  • 2013 Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (43 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (27 results) (of which Invited: 2 results) Remarks (4 results)

  • [Journal Article] Diversity in Guanosine 3',5'-Bisdiphosphate (ppGpp) Sensitivity Among Guanylate Kinases of Bacteria and Plants2014

    • Author(s)
      Nomura, Y., Izumi, A., Fukunaga, Y., Kusumi, K., Iba, K., Watanabe, S., Nakahira, Y., Weber, A.P., Nozawa, A., and Tozawa, Y.
    • Journal Title

      J. Biol. Chem.

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of the chloroplast genetic system during early leaf development in rice2014

    • Author(s)
      Kusumi, K., and Iba, K.
    • Journal Title

      Front Plant Sci.

      Volume: (in press)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] New Approaches to the Biology of Stomatal Guard Cells.2014

    • Author(s)
      Negi, J., Hashimoto-Sugimoto, M., Kusumi, K. and Iba, K.
    • Journal Title

      Plant Cell Physiol.

      Volume: 55 Issue: 2 Pages: 241-250

    • DOI

      10.1093/pcp/pct145

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Diversity in Guanosine 3′,5′-Bisdiphosphate (ppGpp) Sensitivity Among Guanylate Kinases of Bacteria and Plants.2014

    • Author(s)
      Nomura, Y., Izumi, A., Fukunaga, Y. Kusumi, K., Iba, K., Watanabe, S., Nakahira, Y., Weber, A. P., Nozawa, A., and Tozawa, Y.
    • Journal Title

      J. Biol. Chem.

      Volume: 289 Issue: 22 Pages: 15631-15641

    • DOI

      10.1074/jbc.m113.534768

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A Dof Transcription Factor, SCAP1, Is Essential for the Development of Functional Stomata in Arabidopsis2013

    • Author(s)
      Negi, J., Moriwaki, K., Konishi, M., Yokoyama, R., Nakano, T., Kusumi, K., Hashimoto-Sugimoto, M., Schroeder, J.I., Nishitani, K., Yanagisawa, S. and Iba, K.
    • Journal Title

      Current Biology

      Volume: 23 Issue: 6 Pages: 479-484

    • DOI

      10.1016/j.cub.2013.02.001

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Measuring Stomatal Density in Rice2013

    • Author(s)
      Kusumi, K.
    • Journal Title

      Bio-Protocol

    • URL

      http://www.bio-protocol.org/wenzhang.aspx?id=753

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interaction between a plasma membrane-localized ankyrin-repeat protein ITN1 and a nuclear protein RTV12012

    • Author(s)
      Sakamoto, H., Sakata, K., Kusumi, K., Kojima, M., Sakakibara, H. and Iba, K.
    • Journal Title

      Biochemistry Biophysics Research CommunicationBiochemistry Biophysics Research Communication

      Volume: 423 Issue: 2 Pages: 392-397

    • DOI

      10.1016/j.bbrc.2012.05.136

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Increased leaf photosynthesis caused by elevated stomatal conductance in a rice mutant deficient in SLAC1, a guard cell anion channel protein2012

    • Author(s)
      Kusumi, K., Hirotsuka, S., Kumamaru, T., and Iba, K.
    • Journal Title

      J. Exp. Bot.

      Volume: 63 Issue: 15 Pages: 5635-5644

    • DOI

      10.1093/jxb/ers216

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A plastid protein NUS1 is essential for build-up of the genetic system for early chloroplast development under cold stress conditions2011

    • Author(s)
      Kusumi, K., Sakata, C., Nakamura, T., Kawasaki, S., Yoshimura, A., and Iba, K.
    • Journal Title

      Plant J.

      Volume: 68 Issue: 6 Pages: 1039-1050

    • DOI

      10.1111/j.1365-313x.2011.04755.x

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Environmental regulation of stomatal response in the Arabidopsis Cvi-0 ecotype2011

    • Author(s)
      Monda, K., Negi, J., Iio, A., Kusumi, K., Kojima, M., Hashimoto, M., Sakakibara, H. and Iba, K.
    • Journal Title

      Planta

      Volume: 234 Issue: 3 Pages: 555-563

    • DOI

      10.1007/s00425-011-1424-x

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chloroplast biogenesis during the early stage of leaf development in rice2010

    • Author(s)
      Kusumi, et al
    • Journal Title

      Plant Biotechnology

      Volume: 27 Pages: 85-90

    • NAID

      10026411513

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Contribution of chloroplast biogenesis to carbon-nitrogen balance during early leaf development in rice2010

    • Author(s)
      Kusumi, et al
    • Journal Title

      Journal of Plant Research

      Volume: 123 Pages: 617-622

    • NAID

      10027220182

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 葉の発生初期に特異的に働く葉緑体転写制御因子NUS1の解析2014

    • Author(s)
      江原涼美、射場厚、楠見健介
    • Organizer
      九州沖縄植物学会(第64回)
    • Place of Presentation
      琉球大学(沖縄)
    • Year and Date
      2014-05-25
    • Related Report
      2013 Final Research Report
  • [Presentation] イネの気孔閉鎖因子SLAC1の生育段階依存性2014

    • Author(s)
      楠見健介、橋村綾菜、射場厚
    • Organizer
      九州沖縄植物学会(第64回)
    • Place of Presentation
      琉球大学(沖縄)
    • Year and Date
      2014-05-25
    • Related Report
      2013 Final Research Report
  • [Presentation] イネの気孔閉鎖因子SLAC1の機能の生育段階依存性2014

    • Author(s)
      楠見健介,橋村綾菜,射場厚
    • Organizer
      日本植物生理学会第55会年会
    • Place of Presentation
      富山大学(富山)
    • Year and Date
      2014-03-19
    • Related Report
      2013 Final Research Report
  • [Presentation] 植物葉緑体におけるppGppの主要標的分子はグアニル酸キナーゼである2014

    • Author(s)
      野村勇太,泉厚志,福永芳規,楠見健介,射場厚,中平洋一,野澤彰,戸澤譲
    • Organizer
      日本植物生理学会第55会年会
    • Place of Presentation
      富山大学(富山)
    • Year and Date
      2014-03-19
    • Related Report
      2013 Final Research Report
  • [Presentation] 順遺伝子学的手法を用いた孔辺細胞特異的葉緑体機能の解析2014

    • Author(s)
      祢冝淳太郎,楠見健介,宗正晋太郎,藤田麻友美, Julian Schroeder,射場厚
    • Organizer
      日本植物生理学会第55会年会
    • Place of Presentation
      富山大学(富山)
    • Year and Date
      2014-03-19
    • Related Report
      2013 Final Research Report
  • [Presentation] 葉の発生初期に特異的に働く葉緑体転写制御因子NUS1の解析2014

    • Author(s)
      江原涼美、射場厚、楠見健介
    • Organizer
      九州沖縄植物学会 (第64回)
    • Place of Presentation
      琉球大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] イネの気孔閉鎖因子SLAC1の生育段階依存性2014

    • Author(s)
      楠見健介、橋村綾菜、 射場厚
    • Organizer
      九州沖縄植物学会 (第64回)
    • Place of Presentation
      琉球大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 気孔閉鎖因子SLAC1を機能欠失したイネ突然変異株slac1の解析2013

    • Author(s)
      楠見健介,射場厚
    • Organizer
      日本植物生理学会第54回年会
    • Place of Presentation
      岡山大学(岡山)
    • Year and Date
      2013-03-22
    • Related Report
      2013 Final Research Report
  • [Presentation] イネslac1変異株の解析2013

    • Author(s)
      楠見健介
    • Organizer
      Annual FACE Meeting
    • Place of Presentation
      農業環境技術研究所(筑波)
    • Year and Date
      2013-03-05
    • Related Report
      2013 Final Research Report
  • [Presentation] 高等植物の葉緑体機能と葉の分化制御機構2012

    • Author(s)
      楠見健介、堤彩奈、射場厚
    • Organizer
      九州沖縄植物学会例会
    • Place of Presentation
      熊本大学(熊本)
    • Year and Date
      2012-12-08
    • Related Report
      2013 Final Research Report
  • [Presentation] 低温ストレス下において葉緑体rRNAの発現維持に関わるRNA結合蛋白質NUS1の解析2012

    • Author(s)
      楠見健介、堤彩奈、射場厚
    • Organizer
      日本植物学会九州支部(第62回)
    • Place of Presentation
      佐賀大学(佐賀)
    • Year and Date
      2012-05-19
    • Related Report
      2013 Final Research Report
  • [Presentation] 色素体RNA結合タンパク質NUS1は低温ストレス下における葉緑体転写・翻訳装置の発現維持に必須である2012

    • Author(s)
      楠見健介,堤彩奈,射場厚
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学(京都)
    • Year and Date
      2012-03-17
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Presentation] 原核型RNA結合領域を持つ葉緑体タンパク質NUS1の解析2011

    • Author(s)
      堤彩奈、楠見健介、射場厚
    • Organizer
      九州RNAクラブ
    • Place of Presentation
      熊本大学(熊本)
    • Year and Date
      2011-10-18
    • Related Report
      2013 Final Research Report
  • [Presentation] 原核型RNA結合領域を持つ葉緑体タンパク質NUS1の解析2011

    • Author(s)
      堤彩奈
    • Organizer
      九州RNAクラブ
    • Place of Presentation
      熊本大学(熊本)(招待講演)
    • Year and Date
      2011-10-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] 気孔が常時開口するイネ突然変異株osslac1の解析2011

    • Author(s)
      楠見健介、廣塚祥子、熊丸敏博、射場厚
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京大学(東京)
    • Year and Date
      2011-09-18
    • Related Report
      2013 Final Research Report
  • [Presentation] 塩ストレス応答性タンパク質ITN1と転写因子様タンパク質RTV1の相互作用に関する解析2011

    • Author(s)
      坂本光、楠見健介、坂田桂子、小栗秀、射場厚
    • Organizer
      日本植物細胞分子生物学会大会
    • Place of Presentation
      九州大学(福岡)
    • Year and Date
      2011-09-06
    • Related Report
      2013 Final Research Report
  • [Presentation] 気孔閉口因子SLAC1を機能欠失したイネ突然変異株の単離と解析2011

    • Author(s)
      楠見健介、廣塚祥子、射場厚
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学(仙台)
    • Year and Date
      2011-03-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 発生初期ステージのイネの葉におけるC/Nバランス制御と葉緑体分化の寄与2010

    • Author(s)
      廣塚祥子、楠見健介、射場厚
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      中部大学(名古屋)
    • Year and Date
      2010-09-10
    • Related Report
      2013 Final Research Report
  • [Presentation] バクテリア型RNA結合領域を持つ葉緑体タンパク質NUSの解析2010

    • Author(s)
      溝山泰徳、楠見健介、射場厚
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      中部大学(名古屋)
    • Year and Date
      2010-09-10
    • Related Report
      2013 Final Research Report
  • [Presentation] バクテリア型RNA結合領域を持つ葉緑体タンパク質NUSの解析2010

    • Author(s)
      溝山泰徳
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      中部大学(愛知)
    • Year and Date
      2010-09-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] 気孔閉鎖因子SLAC1を機能抑制したイネ突然変異株の解析2010

    • Author(s)
      楠見健介、廣塚祥子、射場厚
    • Organizer
      日本植物学会九州支部(第61回大会)
    • Place of Presentation
      長崎大学(長崎)
    • Year and Date
      2010-05-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 葉緑体分化初期過程を制御する新規色素体RNA結合タンパク質NUS1の解析2010

    • Author(s)
      楠見健介,坂田知佳子,溝山泰徳,射場厚
    • Organizer
      日本植物学会九州支部(第60回大会)
    • Place of Presentation
      九州大学(福岡)
    • Year and Date
      2010-05-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 原核型RNA結合領域を持つ葉緑体タンパク質NUS1の解析2010

    • Author(s)
      楠見健介
    • Organizer
      日本植物学会九州支部(第60回大会)
    • Place of Presentation
      九州大学(福岡)
    • Year and Date
      2010-05-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 葉緑体分化初期過程を制御する色素体RNA結合タンパク質NUS1の解析2010

    • Author(s)
      楠見健介,坂田知佳子,溝山泰徳,射場厚
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本大学(熊本)
    • Year and Date
      2010-03-20
    • Related Report
      2013 Final Research Report
  • [Presentation] 順遺伝子学的手法を用いた孔辺細胞特異的葉緑体機能の解析

    • Author(s)
      祢冝 淳太郎, 楠見 健介, 宗正晋太郎, 藤田麻友美, Julian Schroeder, 射場 厚
    • Organizer
      日本植物生理学会第55会年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 植物葉緑体における ppGpp の主要標的分子はグアニル酸キナーゼである

    • Author(s)
      野村 勇太, 泉 厚志, 福永 芳規, 楠見 健介, 射場 厚, 中平 洋一, 野澤 彰, 戸澤 譲
    • Organizer
      日本植物生理学会第55会年会
    • Place of Presentation
      名古屋大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 高等植物の葉緑体機能と葉の分化制御機構

    • Author(s)
      楠見 健介
    • Organizer
      九州沖縄植物学会例会
    • Place of Presentation
      九州大学
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Remarks]

    • URL

      http://plant.biology.kyushu-u.ac.jp

    • Related Report
      2013 Final Research Report
  • [Remarks] 九州大学大学院・理学研究院・植物生理学講座

    • URL

      http://plant.biology.kyushu-u.ac.jp/Index.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://plant.biology.kyushu-u.ac.jp/Index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://plant.biology.kyushu-u.ac.jp/Index.html

    • Related Report
      2010 Annual Research Report

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Published: 2010-11-30   Modified: 2019-07-29  

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