Function of D-amino acids on plastid division in plants

• Project/Area Number: 22570046
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kumamoto University
• Principal Investigator: TAKANO Hiroyoshi 熊本大学, バイオエレクトリクス研究センター, 教授 (70242104)
• Co-Investigator(Kenkyū-buntansha): TAKACHI Katsuaki 熊本大学, 大学院・自然科学研究科, 准教授 (70515501)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: D-アミノ酸 / コケ植物 / 葉緑体分裂 / オルガネラ / ペプチドグリカン / オルガ

Research Abstract

It is thought that usage of D-amino acids in organisms is restrictive, although D-aminoacids are essential components of bacterial peptidoglycan. We found that plastidpeptidoglycan synthesis pathway remains in the moss Physcomitrella patens. In this study,we analyzed a P. patens homolog of bacterial D-alanine-D-alanine ligase (Ddl). Wegenerated a PpDdl knockout line, and its phenotype showed a few macrochloroplasts.D-alanyl-D-alanine supplemented in a medium could suppress chloroplast phenotype. Theseresults suggested that D-alanyl-D-alanine synthesized with PpDdl is essential forchloroplast division in P. patens.

Research Products

• [Journal Article] Treatmentwith antibiotics that interfere withpeptidoglycan biosynthesis inhibitschloroplast division in the desmi (2012)
  • Author(s): Matsumoto, H., Takechi, K., Sato, H.,Takio, S. and Takano, H
  • Journal Title: Closterium., PlosOne Volume: 7(7)
  • Peer Reviewed
• [Journal Article] Loss of theplastid envelope protein AtLrgB causesspontaneous chlorotic cell death inArabidopsis thaliana (2012)
  • Author(s): Yamaguchi, M., Takechi, K., Myouga, F.,Imura, S., Sato, H., Takio, S., Shinozaki,K. and Takano, H
  • Journal Title: Plant CellPhysiol Volume: 53 Pages: 125-134
  • Peer Reviewed
• [Journal Article] Treatment with antibiotics that interfere with peptidoglycan biosynthesis inhibits chloroplast division in the desmid Closterium. (2012)
  • Author(s): Matsumoto, H.
  • Journal Title: PlosOne Volume: 7 Issue: 7 Pages: 40734-40734
  • DOI: 10.1371/journal.pone.0040734
  • Peer Reviewed
• [Journal Article] Loss of the plastid envelope protein AtLrgB causes spontaneous chlorotic cell death in Arabidopsis thaliana (2012)
  • Author(s): M.Yamaguchi
  • Journal Title: Plant Cell Physiol Volume: 53 Issue: 1 Pages: 125-134
  • DOI: 10.1093/pcp/pcr180
  • Peer Reviewed
• [Journal Article] Three dynamin-related protein 5B genes arerelated to plastid division inPhyscomitrella patens (2011)
  • Author(s): Sakaguchi, E., Takechi, K., Sato, H.,Yamada, T., Takio, S. and Takano, H
  • Journal Title: Plant Sci Volume: 180 Pages: 789-795
  • Peer Reviewed
• [Journal Article] Three dynamin-related protein 5B genes are related to plastid division in Physcomitrella patens (2011)
  • Author(s): E.Sakaguchi
  • Journal Title: Plant Sci Volume: 180 Issue: 6 Pages: 789-795
  • DOI: 10.1016/j.plantsci.2011.02.003
  • Peer Reviewed
• [Journal Article] Three dynamin-related protein 5B genes are related to plastid division in Physcomitrella patens. (2011)
  • Author(s): Sakaguchi
  • Journal Title: Plant Sci. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Mitochondrial fusion andinheritance of the mitochondrial genome (2010)
  • Author(s): Takano, H., Onoue, K. and Kawano, S
  • Journal Title: J. Plant Res Volume: 123 Pages: 131-138
  • Peer Reviewed
• [Journal Article] Chloroplast peptidoglycan (2010)
  • Author(s): Takano, H. and Takechi, K
  • Journal Title: Biochim.Biophys. Acta Volume: 1800 Pages: 144-151
  • Peer Reviewed
• [Journal Article] Chloroplast peptidoglycan. (2010)
  • Author(s): Takano, H.
  • Journal Title: Biochim.Biophys.Acta Volume: 1800 Pages: 144-151
  • Peer Reviewed
• [Journal Article] Mitochondrial fusion and inheritance of the mitochondrial genome. (2010)
  • Author(s): Takano, H.
  • Journal Title: J.Plant Res. Volume: 123 Pages: 131-138
  • NAID: 10027217884
  • Peer Reviewed
• [Presentation] ヒメツリガネゴケにおいて葉緑体分裂に関与するD-アラニン:D-アラニンリガーゼ (2012)
  • Author(s): 谷所幸治
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都)
  • Year and Date: 2012-03-17
• [Presentation] Mur-related genes for peptidoglycan biosynthesis are related to chloroplast division in the moss Physcomitrella patens (2012)
  • Author(s): Takano, H.
  • Organizer: Moss2012
  • Place of Presentation: NY Botanical Garden, NY, USA
• [Presentation] Maintenance of normal stress tolerance in Physcomitrella patens lacking chloroplastic superoxide dismutases (2012)
  • Author(s): Higahsi, Y.
  • Organizer: Moss2012
  • Place of Presentation: NY Botanical Garden, NY, USA
• [Presentation] lesion mimic の形質を示すatlrgBミュータントの解析 (2012)
  • Author(s): 山口瑞樹
  • Organizer: 日本植物学会大会
  • Place of Presentation: 兵庫県立大学、姫路
• [Presentation] ヒメツリガネゴケにおいてMembrane-bound lytic transglycosylase B（MltB）相同遺伝子は葉緑体の分裂・形態形成に関与する (2012)
  • Author(s): 宇都宮英恵
  • Organizer: 日本植物学会
  • Place of Presentation: 兵庫県立大学、姫路
• [Presentation] 緑化した葉の一部が白色化していくシロイヌナズナapg17タグラインの解析 (2011)
  • Author(s): 山口瑞貴
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京大学(東京)
  • Year and Date: 2011-09-17
• [Presentation] Involvement of microRNA in copper-deficiency induced repression of chloroplastic CuZn-superoxide dismutase genes in Physcomitrella patens (2011)
  • Author(s): Y.Higashi
  • Organizer: Moss2011
  • Place of Presentation: Black Forest, Germany
  • Year and Date: 2011-09-13
• [Presentation] 緑化した葉の一部が白色化するシロイヌナズナapg17タグラインの解析 (2011)
  • Author(s): 山口瑞貴
  • Organizer: 日本植物学会九州支部大会
  • Place of Presentation: 長崎大学(長崎)
  • Year and Date: 2011-05-21
• [Presentation] 真正粘菌の原始的な性とミトコンドリアの遺伝 (2010)
  • Author(s): 森山陽介
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学・春日井市
  • Year and Date: 2010-09-09
• [Presentation] Three Dynamin-Related Protein (DRP)5B genes are redundant for plastid divisions in P.patens (2010)
  • Author(s): Takano, H.
  • Organizer: Moss2010
  • Place of Presentation: 水の詩ホテル・札幌
  • Year and Date: 2010-07-21
• [Presentation] Two MurA genes for the peptidoglycan biosynthesis are related to chloroplast division in the moss Physcomitrella patens. (2010)
  • Author(s): Tanidokoro, K.
  • Organizer: Moss2010
  • Place of Presentation: 水の詩ホテル・札幌
  • Year and Date: 2010-07-21
• [Presentation] Evolution of Chloroplast division machinery with peptidoglycan (2010)
  • Author(s): Takano, H.
  • Organizer: Seminar in Inner Mongolia University
  • Place of Presentation: 内蒙古大学・フフホト・中国
  • Year and Date: 2010-06-23