Application of gene targeting by homologous recombination as a biothecnological tool for rice functional genomics.

Research Project

Project/Area Number	22570049
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Plant molecular biology/Plant physiology
Research Institution	University of Shizuoka
Principal Investigator	JOHZUKA Yasuyo 静岡県立大学, 融合科学研究科(研究院), 博士研究員 (60517887)
Co-Investigator(Kenkyū-buntansha)	NOGUCHI Hiroshi 静岡県立大学, 薬学部, 教授 (60126141) IIDA Shigeru 静岡県立大学, 大学院・生活科学健康科・薬学研究科, 特任教授 (30012777)
Project Period (FY)	2010 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000) Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	遺伝子ターゲティング / 相同組換え / 変異導入 / 植物 / イネ / 形質転換 / バイオテクノロジー / 遺伝子 / バイオテクノロジ
Research Abstract	Rice, an important food, is the staple grain for one-half of the world’s population. We have succeeded in the development of a gene-targeting method which involves the genetic modification of rice DNA. Aiming at wide use of targeting of a higher plant, we developed making suitable recombination enzyme (λRed system) , and making targeting frequency increase. The vectors for assay for measuring targeting frequency were also developed.