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Application of gene targeting by homologous recombination as a biothecnological tool for rice functional genomics.

Research Project

Project/Area Number 22570049
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionUniversity of Shizuoka

Principal Investigator

JOHZUKA Yasuyo  静岡県立大学, 融合科学研究科(研究院), 博士研究員 (60517887)

Co-Investigator(Kenkyū-buntansha) NOGUCHI Hiroshi  静岡県立大学, 薬学部, 教授 (60126141)
IIDA Shigeru  静岡県立大学, 大学院・生活科学健康科・薬学研究科, 特任教授 (30012777)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords遺伝子ターゲティング / 相同組換え / 変異導入 / 植物 / イネ / 形質転換 / バイオテクノロジー / 遺伝子 / バイオテクノロジ
Research Abstract

Rice, an important food, is the staple grain for one-half of the world’s population. We have succeeded in the development of a gene-targeting method which involves the genetic modification of rice DNA. Aiming at wide use of targeting of a higher plant, we developed making suitable recombination enzyme (λRed system) , and making targeting frequency increase. The vectors for assay for measuring targeting frequency were also developed.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (1 results)

All 2011

All Journal Article (1 results) (of which Peer Reviewed: 1 results)

  • [Journal Article] The molecular basis of incomplete dominance at the A locus of CHS-D in the common morning glory, Ipomoea purpurea.2011

    • Author(s)
      Y.Johzuka-Hisatomi, H.Noguchi, S.Iida
    • Journal Title

      Journal of Plant Research

      Volume: 124 Pages: 299-304

    • NAID

      10027868379

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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