Novel translation mechanism in chloroplasts
| Project/Area Number |
22570050
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Nagoya City University |
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Principal Investigator |
SUGIURA Masahiro 名古屋市立大学, 大学院・システム自然科学研究科, 名誉教授 (80027044)
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| Co-Investigator(Renkei-kenkyūsha) |
YUKAWA Maki 名古屋市立大学, 大学院・システム自然科学研究科, 研究員 (00448705)
NAKAMURA Masayuki 名古屋市立大学, 大学院・システム自然科学研究科, 研究員 (60322145)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 葉緑体 / 翻訳 / mRNA / タバコ / 重複遺伝子 / in vitro系 |
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| Research Abstract |
The chloroplast NAD(P)H dehydrogenase (NDH) C (ndhC) and ndhK genes partially overlap and are cotranscribed. We previously reported that the tobacco ndhC/K genes are translationally coupled but produce NdhC and NdhK subunits in similar amounts. This finding suggested that the ndhK cistron is translated not only from the ndhC 5′UTR but also by an additional pathway. Using an in vitro translation system from tobacco chloroplasts, we found that free ribosomes enter at an internal AUG start codon located in the middle of the upstream ndhC cistron.
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Report
(4 results)
Research Products
(67 results)
