Novel translation mechanism in chloroplasts

• Project/Area Number: 22570050
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya City University
• Principal Investigator: SUGIURA Masahiro 名古屋市立大学, 大学院・システム自然科学研究科, 名誉教授 (80027044)
• Co-Investigator(Renkei-kenkyūsha): YUKAWA Maki 名古屋市立大学, 大学院・システム自然科学研究科, 研究員 (00448705) ; NAKAMURA Masayuki 名古屋市立大学, 大学院・システム自然科学研究科, 研究員 (60322145)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 葉緑体 / 翻訳 / mRNA / タバコ / 重複遺伝子 / in vitro系

Research Abstract

The chloroplast NAD(P)H dehydrogenase (NDH) C (ndhC) and ndhK genes partially overlap and are cotranscribed. We previously reported that the tobacco ndhC/K genes are translationally coupled but produce NdhC and NdhK subunits in similar amounts. This finding suggested that the ndhK cistron is translated not only from the ndhC 5′UTR but also by an additional pathway. Using an in vitro translation system from tobacco chloroplasts, we found that free ribosomes enter at an internal AUG start codon located in the middle of the upstream ndhC cistron.

Research Products

• [Journal Article] An additional pathway to translate the downstream ndhK cistron in partially overlapping ndhC-ndhK mRNAs in chloroplasts (2013)
  • Author(s): Yukawa, M. and M. Sugiura
  • Journal Title: Proc. Natl. Acad. Sci. USA. Volume: 110 Issue: 14 Pages: 5701-5706
  • DOI: 10.1073/pnas.1219914110
  • Peer Reviewed
• [Journal Article] The context of transcription start site regions is crucial for transcription of a plant tRNALys(UUU) gene group both in vitro and in vivo. (2013)
  • Author(s): Yukawa, Y., K. Akama, K. Noguchi, M. Komiya and M. Sugiura
  • Journal Title: Gene Volume: 512 Issue: 2 Pages: 286-293
  • DOI: 10.1016/j.gene.2012.10.022
  • Peer Reviewed
• [Journal Article] A novel hypoxic stress-responsive long non-coding RNA transcribed by RNA polymerase III in Arabidopsis. (2012)
  • Author(s): Wu, J., T. Okada, T. Fukushima, T. Tsudzuki, M. Sugiura and Y. Yukawa
  • Journal Title: RNA Biology Volume: 9 Pages: 320-313
  • Peer Reviewed
• [Journal Article] A novel hypoxic stress-responsive long non-coding RNA transcribed by RNA polymerase III in Arabidopsis (2012)
  • Author(s): Wu, J., T.Okada, T.Fukushima, T.Tsudzuki, M.Sugiura, Y.Yukawa
  • Journal Title: RNA Biology Volume: Vol.9 Pages: 302-313
  • Peer Reviewed
• [Journal Article] Translation of partially overlapping psbD-psbC mRNAs in chloroplasts: the role of 5'-processing and translational coupling. (2011)
  • Author(s): Adachi, Y., H. Kuroda, Y. Yukawa and M. Sugiura
  • Journal Title: Nucl. Acids. Res. Volume: 40 Issue: 7 Pages: 3152-3158
  • DOI: 10.1093/nar/gkr1185
  • Peer Reviewed
• [Journal Article] The downstream atpE cistron is efficiently translated via its own cis-element in partially overlapping atpB-atpE dicistronic mRNAs in chloroplasts. (2011)
  • Author(s): Suzuki, H., H. Kuroda, Y. Yukawa and M. Sugiura
  • Journal Title: Nucl. Acids. Res Volume: 39 Issue: 21 Pages: 9405-9412
  • DOI: 10.1093/nar/gkr644
  • Peer Reviewed
• [Journal Article] A common sequence motif involved in selectoin of transcription start sites of Arabidopsis and budding yeast tRNA genes. (2011)
  • Author(s): Yukawa, Y., G. Dieci, M. Alzapiedi, A. Hiraga, K. Hirai, Y. Yamamoto and M. Sugiura
  • Journal Title: Genomics Volume: 97 Issue: 3 Pages: 166-172
  • DOI: 10.1016/j.ygeno.2010.12.001
  • Peer Reviewed
• [Journal Article] Translation efficiencies of synonymous codons for arginine differ widely and are not correlated with codon usage in chloroplasts (2011)
  • Author(s): Nakamura, M. and M. Sugiura
  • Journal Title: Gene Volume: 472 Issue: 1-2 Pages: 50-54
  • DOI: 10.1016/j.gene.2010.09.008
  • Peer Reviewed
• [Journal Article] Translation efficiencies of synonymous codons for arginine differ widely and are not correlated with codon usage in chloroplasts. (2011)
  • Author(s): Nakamura, M., M.Sugiura
  • Journal Title: Gene Volume: Vol.472 Pages: 50-54
  • Peer Reviewed
• [Journal Article] A common sequence motif involved in selection of transcription start sites of Arabidopsis and budding yeast tRNA genes. (2011)
  • Author(s): Yukawa, Y., G.Dieci, M.Sugiura, et al
  • Journal Title: Genomics Volume: Vol.97 Pages: 166-172
  • Peer Reviewed
• [Journal Article] An organellar maturase associates with multiple group II introns. (2010)
  • Author(s): Zoschke, R., M. Nakamura, K. Liere, M. Sugiura, T. Borner and C. Schmitz-Linneweber
  • Journal Title: Proc. Natle. Acad. Sci. USA Volume: 107 Issue: 7 Pages: 3245-3250
  • DOI: 10.1073/pnas.0909400107
  • Peer Reviewed
• [Journal Article] An organellar maturase associates with multiple group II introns. (2010)
  • Author(s): Zoschke, R., M.Nakamura, K.Liere, M.Sugiura, et al
  • Journal Title: Proc.Natle.Acad.Sci.USA Volume: Vol.107 Pages: 3245-3250
  • Peer Reviewed
• [Presentation] An Arabidopsis long non-coding RNA transcribed by RNA polymerase III responds to hypoxic stress (2013)
  • Author(s): Yukawa, Y., J. Wu, T. Okada, T. Fukushima and M. Sugiura
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山
• [Presentation] Analysis of the translational control mechanism on Arabidopsis rbcS mRNAs (2013)
  • Author(s): Yukawa, M. and M. Sugiura
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山
• [Presentation] タバコ葉緑体rps16 mRNA の翻訳抑制因子の探索 (2013)
  • Author(s): 中邨真之、杉浦昌弘
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山
• [Presentation] An arabidopsis long non-coding RNAtranscribed by RNA polymerase III responds to hypoxic stress (2013)
  • Author(s): Yukawa, Y., J. Wu, T. Okada, T. Fukushima, M. Sugiura
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学（岡山県）
• [Presentation] Analysis of the translational control mechanism on Arabidopsis rbcS mRNAs (2013)
  • Author(s): Yukawa, M. and M. Sugiura
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学（岡山県）
• [Presentation] タバコ葉緑体rps16 mRNAの翻訳抑制因子の探索 (2013)
  • Author(s): 中邨真之、杉浦昌弘
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学（岡山県）
• [Presentation] タバコ葉緑体ndhC-ndhK mRNA に見られるオルタナティブイニシエーションと終止コドンに依存した翻訳開始 (2012)
  • Author(s): 湯川眞希、杉浦昌弘
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] タバコ葉緑体rps16 mRNA の翻訳抑制機構の解析 (2012)
  • Author(s): 中邨真之、杉浦昌弘
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Translation inhibition of rps16 mRNA in tobacco chloroplasts is caused by 5'untranslated region (2012)
  • Author(s): Nakamura, M. and M. Sugiura
  • Organizer: Godon Research Conference Mitochondria & Chloroplasts.
  • Place of Presentation: スミスフィールド・アメリカ
• [Presentation] タバコ葉緑体rps16 mRNAの翻訳抑制のin vitro解析 (2012)
  • Author(s): 中邨真之、杉浦昌弘
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
• [Presentation] Translation inhibition of rps16 mRNA in tobacco chloroplasts is caused by 5’untranslated region. (2012)
  • Author(s): Nakamura, M. and M. Sugiura
  • Organizer: Gorden Research Conference “Mitochondria & Chloroplasts”
  • Place of Presentation: Smithfield, RI, USA
• [Presentation] タバコ葉緑体ndhC-K mRNAに見られるオルタナティブイニシエーションと終止コドンに依存した翻訳開始 (2012)
  • Author(s): 湯川眞希、杉浦昌弘
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Presentation] タバコ葉緑体rps16 mRNAの翻訳抑制機構の解析 (2012)
  • Author(s): 中邨真之、杉浦昌弘
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Presentation] タバコ葉緑体rps16 mRNAの翻訳抑制のin vitro解析 (2012)
  • Author(s): 中邨真之、杉浦昌弘
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都府)
• [Presentation] Characterization of a novel long non-coding RNA transcribed by RNA polymerase III in Arabidopsis (2011)
  • Author(s): Juan Wu、岡田敏浩、福島徹、續伯彦、杉浦昌弘、湯 川 泰
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Analysis of the gene for choloroplast 30S ribosomal protein S16 in tobacco (2011)
  • Author(s): Nakamura, M. and M. Sugiura
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] タバコ葉緑体ndhC-K mRNAに見られる新しい終止コドン依存型翻訳 (2011)
  • Author(s): 湯川眞希、杉浦昌弘
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] 植物培養細胞からの細胞質 in vitro 翻訳解析法の開発 (2011)
  • Author(s): Xin He、山 本 裕 子 、湯川眞希、杉浦昌弘、湯川 泰
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] タバコ葉緑体 in vitro 翻訳系を用いたrbcL 翻訳開始機構の解析 (2011)
  • Author(s): 黒田恵、黒田洋詩、湯川泰、杉浦昌弘
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] タバコ葉緑体 psbD-psbC 発現における翻訳開始機構の解析 (2011)
  • Author(s): 足達由佳、黒田洋詩、湯川泰、杉浦昌弘
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] 顕花植物光合成遺伝子の光依存転写制御の in vitro 解析 (2011)
  • Author(s): 宮崎綾香、岩田真也、杉浦昌弘、湯川泰
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] In vitro 転写系による GST およびノンコーディング RNA 遺伝子転写の相互作用 (2011)
  • Author(s): 山本恵子、杉浦昌弘、湯川泰
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] rps16 gene in tobacco choloroplasts is well transcribed and its transcripts is correctly spliced, but mRNA is not translated (2011)
  • Author(s): Nakamura, M. and M. Sugiura
  • Organizer: The EMBO Meeting 2011
  • Place of Presentation: ウィーン・オーストリア
• [Presentation] rps16 gene in tobacco choloroplasts is well transcribed and its transcripts is correctly spliced, but mRNA is not translated (2011)
  • Author(s): M.Nakamura, M.Sugiura
  • Organizer: The EMBO Meeting 2011
  • Place of Presentation: Austria Center Vienna (Vienna)
• [Presentation] In vitro転写系によるGSTおよびノンコーディングRNA遺伝子転写の相互作用 (2011)
  • Author(s): 山本恵子、杉浦昌弘、湯川泰
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] 顕花植物光合成遺伝子の光依存転写制御のin vitro解析 (2011)
  • Author(s): 宮崎綾香、岩田真也、杉浦昌弘、湯川泰
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] タバコ葉緑体psbD-psbC発現における翻訳開始機構の解析 (2011)
  • Author(s): 足達由佳、黒田洋詩、湯川泰、杉浦昌弘
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] タバコ葉緑体in vitro翻訳系を用いたrbcL翻訳開始機構の解析 (2011)
  • Author(s): 黒田恵、黒田洋詩、湯川泰、杉浦昌弘
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] 植物培養細胞からの細胞質in vitro翻訳解析法の開発 (2011)
  • Author(s): Xin He、山本裕子、湯川眞希、杉浦昌弘、湯川泰
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] タバコ葉緑体ndhC-K mRNAに見られる新しい終止コドン依存型翻訳 (2011)
  • Author(s): 湯川眞希、杉浦昌弘
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] Analysis of the gene for choloroplast 30S ribosomal protein S16 in tobacco (2011)
  • Author(s): M.Nakamura, M.Sugiura
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] Characterization of a novel long non-coding RNA transcribed by RNA polymerase III in Arabidopsis (2011)
  • Author(s): Juan Wu、岡田敏浩、福島徹、續伯彦、杉浦昌弘、湯川泰
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] Requirements for translation initiation of rps2 mRNA in tobacco chloroplasts (2010)
  • Author(s): Kuroda, H., Y. Adachi, S. Ozawa, Y. Takahashi, Y. Yukawa, and M. Sugiura
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会合同大会
  • Place of Presentation: 神戸
• [Presentation] シロイヌナズナにおけるUSE 結合タンパク質複合体の単離解析 (2010)
  • Author(s): 町田瑠璃子、柴田恵利、大谷美沙都、杉山宗隆、杉浦昌弘、湯川泰
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会合同大会
  • Place of Presentation: 神戸
• [Presentation] Functional analysis of novel small RNA in Arabidopsis thaliana (2010)
  • Author(s): Wu J., T. Okata M. Sugiura and Y. Yukawa
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会合同大会
  • Place of Presentation: 神戸
• [Presentation] シロイヌナズナ光合成遺伝子の光依存転写制御の解析 (2010)
  • Author(s): 宮崎綾香、岩田真也、杉浦昌弘、湯川泰
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会合同大会
  • Place of Presentation: 神戸
• [Presentation] Translation efficiency of rps16 mRNA in tobacco chloroplasts (2010)
  • Author(s): 中邨真之、杉浦昌弘
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会合同大会
  • Place of Presentation: 神戸
• [Presentation] 葉緑体mRNAの同義コドンと翻訳活性 (2010)
  • Author(s): 中邨真之、杉浦昌弘
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 春日井
• [Presentation] Translation activity of rps16 mRNA in tobacco chloroplasts (2010)
  • Author(s): Nakamura, M. and M. Sugiura
  • Organizer: 11th Internatoinal Colloquium on Endocytobiology and Symbiosis
  • Place of Presentation: トロムソ・ノルウェー
• [Presentation] Chloroplast- and Nuclear-encoded rps16 genes in tobacco (2010)
  • Author(s): Nakamura, M. and M. Sugiura
  • Organizer: Gorden Research Conference "Mitochondria & Chloroplasts"
  • Place of Presentation: ルッカ・イタリア
• [Presentation] Comparative analysis of the translation efficiencies between rps2 and rps16 mRNAs in tobacco chloroplasts (2010)
  • Author(s): Nakamura, M. and M. Sugiura
  • Organizer: FESPB 2010
  • Place of Presentation: バレンシア・スペイン
• [Presentation] Termination-dependent translation of chloroplast ndhK mRNA (2010)
  • Author(s): Sugiura, M. and M. Yukawa
  • Organizer: FESPB 2010
  • Place of Presentation: バレンシア・スペイン
• [Presentation] Translation activity of rps16 mRNA in tobacco chloroplasts. (2010)
  • Author(s): Nakamura, M., M.Sugiura
  • Organizer: 11^<th> Internatoinal Colloquium on Endocytobiology and Symbiosis
  • Place of Presentation: ノルウェー・トロムソ市
• [Presentation] Termination-dependent translation of chloroplast ndhK mRNA. (2010)
  • Author(s): Sugiura, M., M.Yukawa
  • Organizer: FESPB 2010
  • Place of Presentation: スペイン・バレンシア市
• [Presentation] Comparative analysis of the translation efficiencies between rps2 and rps16 mRNAs in tobacco chloroplasts. (2010)
  • Author(s): Nakamura, M., M.Sugiura
  • Organizer: FESPB 2010
  • Place of Presentation: スペイン・バレンシア市
• [Presentation] Chloroplast- and Nuclear-encoded rps16 genes in tobacco. (2010)
  • Author(s): Nakamura, M., M.Sugiura
  • Organizer: Gorden Research Conference "Mitochondria & Chloroplasts"
  • Place of Presentation: イタリア・ルッカ市
• [Presentation] 葉緑体mRNAの同義コドンと翻訳活性 (2010)
  • Author(s): 中邨真之、杉浦昌弘
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学(愛知県)
• [Presentation] Translation efficiency of rps16 mRNA in tobacco chloroplasts. (2010)
  • Author(s): 中邨真之、杉浦昌弘
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
• [Presentation] シロイヌナズナ光合成遺伝子の光依存転写制御の解析 (2010)
  • Author(s): 宮崎綾香、杉浦昌弘, 他
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
• [Presentation] Functional analysis of novel small RNA in Arabidopsis thaliana. (2010)
  • Author(s): Wu J., T.Okata M.Sugiura, et al
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
• [Presentation] シロイヌナズナにおけるUSE結合タンパク質複合体の単離解析 (2010)
  • Author(s): 町田瑠璃子、杉浦昌弘, 他
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
• [Presentation] Requirements for translation initiation of rps2 mRNA in tobacco chloroplasts. (2010)
  • Author(s): 黒田洋詩、杉浦昌弘, 他
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
• [Remarks]
  • URL: http://www.gene.nagya-u.ac.jp/~sugiurag/
• [Remarks]
  • URL: http://www.nsc.nagoya-cu.ac.jp/profile/sugiura.html
• [Remarks]
  • URL: http://www.nsc.nagoya-cu.ac.jp/profile/sugiura.html