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Functional analysis of cytokinin signaling pathway in defense response of rice

Research Project

Project/Area Number 22570057
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

JIANG Chang-jie  独立行政法人農業生物資源研究所, 耐病性作物研究開発ユニット, 上級研究員 (80370659)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords防御応答 / サイトカイニン / サリチル酸 / イネ / いもち病 / イモチ病
Research Abstract

Hormone crosstalk is pivotal in plant.pathogen interactions. In this study, we found that blast infection to rice seedlings lead to massive accumulation of N^6-(Δ^2-isopentenyl) adenine (iP), iP riboside (iPR) and iPR 5′-phosphates (iPRP) in leaf blades. Consistent with this, cytokinin (CK) signaling was activated around the infection sites as shown by histochemical staining for β-glucuronidase activity driven by a CK-responsive OsRR6 promoter. A diverse CK species were also detected in the hyphae (mycelium), conidia and culture filtrates of blast fungus, indicating that M. oryzae is capable of production, as well as hyphal secretion of CKs. Co-treatment of leaf blades with CK and salicylic acid (SA), but not with either one alone, markedly induced pathogenesis-related genes OsPR1b and PBZ1 (probenazole-induced protein 1). These effects were diminished by RNAi-knockdown of OsNPR1 or WRKY45, the key regulators of SA signaling pathway in rice, indicating that the effects of CKs depend on these two regulators. Taken together, our data imply a coevolutionary rice.M. oryzae interaction, wherein M. oryzae probably elevates rice CK levels for its own benefits such as nutrient translocation. Rice plants, on the other hand, sense it as an infection signal and activate defense reactions through the synergistic action with SA.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (6 results)

All 2013 2012 2011 2010

All Journal Article (4 results) (of which Peer Reviewed: 2 results) Presentation (2 results)

  • [Journal Article] Cytokinins act synergistically with salicylic acid to activate defense gene expression in rice2013

    • Author(s)
      Jiang CJ, Shimono M, Sugano S, Kojima M, Liu X, Inoue H, Sakakibara H and Tatatsuji H
    • Journal Title

      Cytokinins act synergistically with salicylic acid to activate defense gene expression in rice.

      Volume: 26 Pages: 287-296

    • Related Report
      2012 Final Research Report
  • [Journal Article] Cytokinins Act Synergistically with Salicylic Acid to Activate Defense Gene Expression in Rice2013

    • Author(s)
      Chang-Jie Jiang, Masaki Shimono, Shoji Sugano, Mikiko Kojima, Xinqiong Liu, Haruhiko Inoue, Hitoshi Sakakibara, and Hiroshi Takatsuji
    • Journal Title

      Molecular Plant-Microbe Interactions (MPMI)

      Volume: 26 Issue: 3 Pages: 287-296

    • DOI

      10.1094/mpmi-06-12-0152-r

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Induction of resistance to Phytophthora sojae in soyabean (Glycine max) by salicylic acid and ethylene2012

    • Author(s)
      Sugano S, Sugimoto T, Takatsuji T and Jiaing CJ
    • Journal Title

      Plant Pathol

      Volume: 62 Issue: 5 Pages: 1048-1056

    • DOI

      10.1111/ppa.12011

    • Year and Date
      2012-11-07
    • Related Report
      2012 Final Research Report
  • [Journal Article] Abscisic Acid Interacts Antagonistically with Salicylic Acid Signaling Pathway in Rice-Magnaporthe grisea Interaction.2010

    • Author(s)
      Chang-Jie Jiang, et al.
    • Journal Title

      Molecular Plant-Microbe Interactions

      Volume: 6 Pages: 791-798

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Cytokinins act synergistically with salicylic acid to activate defense gene expression in rice2011

    • Author(s)
      Jiang CJ, Shimono M, Sugano S, Kojima M, Liu X, Inoue H, Sakakibara H, Takatsuji H
    • Organizer
      9th International Symposium of Rice Functional Genomics
    • Place of Presentation
      Taibei
    • Related Report
      2012 Final Research Report
  • [Presentation] Cytokinins act synergistically with salicylic acid to activate defense gene expression in rice2011

    • Author(s)
      Chang-Jie Jiang, et al
    • Organizer
      9th International Symposium of Rice Functional Genomics
    • Place of Presentation
      台湾・台北
    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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