Analysis of the phototropin signaling pathways controlling the phototropic responses.

• Project/Area Number: 22570058
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Niigata University
• Principal Investigator: SAKAI Tatsuya 新潟大学, 自然科学系, 准教授 (10360554)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 植物 / 光 / オーキシン / 環境応答 / 分子遺伝学 / シグナル伝達 / 遺伝学 / 環境 / 生理学

Research Abstract

We have studied about the molecular mechanisms controlling the phototropic responses in plants. Our analyses have indicated that the blue-light photoreceptor phototropin stabilizes the signaling transducer RPT2 proteins, which are required for phototropism and degraded under the dark condition. Furthermore, we have revealed that PIN auxin efflux carriers are necessary for pulse-induced but not continuous light-induced phototropism in Arabidopsis.

Research Products

• [Journal Article] Role of RPT2 in leaf positioning and flattening and a possible inhibition of phot2 signaling by phot1 (2013)
  • Author(s): Harada A, Takemiya A, Inoue S, Sakai T and Shimazaki K
  • Journal Title: Plant Cell Physiol. Volume: 54 Issue: 1 Pages: 36-47
  • DOI: 10.1093/pcp/pcs094
  • NAID: 10031145648
  • Peer Reviewed
• [Journal Article] PIN Auxin Efflux Carriers Are Necessary for Pulse-Induced But Not Continuous Light-Induced Phototropism in Arabidopsis (2013)
  • Author(s): Ken Haga
  • Journal Title: Plant Physiology Volume: 160 Pages: 763-776
  • Peer Reviewed
• [Journal Article] Differential roles of auxin efflux carrier PIN proteins in hypocotyl phototropism of etiolated Arabidopsis seedlings depend on direction of light stimulus (2012)
  • Author(s): Ken Haga and Tatsuya Sakai
  • Journal Title: Plant Signaling Behavi Volume: 8 Issue: 1 Pages: e22556-e22556
  • DOI: 10.4161/psb.22556
  • Peer Reviewed
• [Journal Article] PINauxin efflux carriers are necessary for pulse-induced but not continuous light-induced phototropism in Arabidopsis (2012)
  • Author(s): Ken Haga and Tatsuya Sakai
  • Journal Title: Plant Physiol Volume: 160 Issue: 2 Pages: 763-776
  • DOI: 10.1104/pp.112.202432
  • Peer Reviewed
• [Journal Article] Molecular genetic analysis of phototropism in Arabidopsis (2012)
  • Author(s): Tatsuya Sakai and Ken Haga
  • Journal Title: Plant Cell Physiol Volume: 53 Issue: 9 Pages: 1517-1534
  • DOI: 10.1093/pcp/pcs111
  • NAID: 10031052991
  • Peer Reviewed
• [Journal Article] The main auxin biosynthesis pathway in Arabidopsis (2011)
  • Author(s): Mashiguchi, K., Tanaka, K., Sakai, T., Sugawara, S., Kawaide, H., Natsume, M., Hanada, H., Yaeno, T., Shirasu, K., Yao, H., Mc Steen, P., Zhao, Y., Hayashi, K., Kamiya, Y., Kasahara, H.
  • Journal Title: Proc.Natl.Acad.Sci.USA. Volume: 108 Issue: 45 Pages: 18512-18517
  • DOI: 10.1073/pnas.1108434108
  • Peer Reviewed
• [Journal Article] Alkoxy-auxins are selective inhibitors of auxin transport mediated by PIN, ABCB and AUX1 transporters (2011)
  • Author(s): Etsuko Tsuda, Haibing Yang, Takeshi Nishimura, Yukiko Uehara, Tatsuya Sakai, Masahiko Furutani, Tomokazu Koshiba, Masakazu Hirose, Hiroshi Nozaki, Angus S. Murphy, and Ken-ichiro Hayashi
  • Journal Title: J. Biol. Chem Volume: 286 Issue: 3 Pages: 2354-2364
  • DOI: 10.1074/jbc.m110.171165
  • Peer Reviewed
• [Presentation] Phototropism and auxin transports. Tropism symposium (2013)
  • Author(s): Tatsuya Sakai and Ken Haga
  • Organizer: Japanese Society of Plant Physiology, Okayama
  • Place of Presentation: 岡山大学
  • Year and Date: 2013-03-21
• [Presentation] RPT2はphot1による葉の定位と平滑化に関与している (2013)
  • Author(s): 原田明子、武宮淳史、井上晋一郎、酒井達也、島崎研一郎
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 岡山、岡山大学
  • Year and Date: 2013-03-21
• [Presentation] シロイヌナズナ胚軸の一次正光屈性および二次正光屈性におけるオーキシン排出輸送体PINの役割 (2013)
  • Author(s): 芳賀健、酒井達也
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山、岡山大学
  • Year and Date: 2013-03-21
• [Presentation] Phototropism and auxin transports. (2013)
  • Author(s): Tatsuya Sakai
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山大学
  • Invited
• [Presentation] RPT2 は phot1 による葉の定位と平滑化に関与している (2013)
  • Author(s): 原田明子
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山大学
• [Presentation] シロイヌナズナ胚軸の一次正光屈性および二次正光屈性におけるオーキシン排出輸送体 PIN の役割 (2013)
  • Author(s): 芳賀健
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山大学
• [Presentation] NIMA関連キナーゼは花成制御因子 FT、TFL1 と相互作用する (2013)
  • Author(s): 高谷彰吾
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山大学
• [Presentation] PIN Auxin Efflux Carriers are Necessary for Pulse-Induced but not Continuous-Light-Induced Phototropism in Arabidopsis (2012)
  • Author(s): Ken Haga and Tatsuya Sakai
  • Organizer: Auxin 2012
  • Place of Presentation: Hawaii, USA
  • Year and Date: 2012-12-10
• [Presentation] PIN-mediated hypocotyl phototropism in Arabidopsis (2012)
  • Author(s): Ken Haga, Tatsuya Sakai
  • Organizer: The 1st International Symposium on Plant Environmental Sensing
  • Place of Presentation: Nara, Japan
  • Year and Date: 2012-03-20
• [Presentation] TAAとYUCによるインドール-3-酢酸の生合成 (2012)
  • Author(s): 増口潔、田中慧太、酒井達也、菅原聡子、夏目雅裕、川出洋、花田篤志、八丈野孝、白須賢、Hong Yao, Paula McSteen、Yunde Zhao、林謙一郎、神谷勇治、笠原博幸
  • Organizer: 日本植物生理学会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-17
• [Presentation] 光屈性シグナル伝達因子RPT2のphot1による発現制御機構の解析 (2012)
  • Author(s): 酒井達也、槌田(間山)智子、上原由紀子、松井南
  • Organizer: 日本植物生理学会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-17
• [Presentation] オーキシン排出体PINを介したシロイヌナズナ胚軸の光屈性 (2012)
  • Author(s): 芳賀健、酒井達也
  • Organizer: 日本植物生理学会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-17
• [Presentation] 光屈性シグナル伝達因子RPT2のphot1による発現制御機構の解析 (2012)
  • Author(s): 酒井達也、槌田(間山)智子、上原由紀子、松井南
  • Organizer: 日本植物生理学会
  • Place of Presentation: 京都、京都産業大学
  • Year and Date: 2012-03-16
• [Presentation] オーキシン排出体PINを介したシロイヌナズナ胚軸の光屈性 (2012)
  • Author(s): 芳賀健、酒井達也
  • Organizer: 日本植物生理学会
  • Place of Presentation: 京都、京都産業大学
  • Year and Date: 2012-03-16
• [Presentation] PIN-mediated hypocotyl phototropism in Arabidopsis (2012)
  • Author(s): Ken Haga, Tatsuya Sakai
  • Organizer: The 1st International Symposium on Plant Environmental Sensing. Nara, Japan
  • Place of Presentation: 奈良県新公会堂
  • Year and Date: 2012-03-12
• [Presentation] PIN Auxin Efflux Carriers are Necessary for Pulse-Induced but not Continuous-Light-InducedPhototropism in Arabidopsis. (2012)
  • Author(s): Ken Haga
  • Organizer: Auxin 2013
  • Place of Presentation: ハワイ
• [Presentation] シロイヌナズナのインドール-3-酢酸生合成経路の解明 (2011)
  • Author(s): 増口潔、田中慧太、酒井達也、菅原聡子、夏目雅裕、川出洋、花田篤志、林謙一郎、神谷勇治、笠原博幸
  • Organizer: 植物化学調節学会年会
  • Place of Presentation: 宇都宮
  • Year and Date: 2011-11-01
• [Presentation] Role of auxin and gibberellin in the phototropic respons (2011)
  • Author(s): Tatsuya Sakai, Tomoko Tsuchida-Mayama, Akitomo Nagashima, Ken Haga, Akiko Hanada, Yukiko Uehara, Kenishiro Hayashi, Shinjiro Yamaguchi
  • Organizer: Asia and Oceania Conference for Photobiology
  • Place of Presentation: 奈良(招待講演)
  • Year and Date: 2011-07-31
• [Presentation] Role of auxin and gibberellin in the phototropic respons. Asia and Oceania Conference for Photobiology (2011)
  • Author(s): Tatsuya Sakai, Tomoko Tsuchida-Mayama, Akitomo Nagashima, Ken Haga, Akiko Hanada, Yukiko Uehara, Kenishiro Hayashi, Shinjiro Yamaguchi
  • Organizer: Nara, Japan. Invited
  • Place of Presentation: 奈良県新公会堂
  • Year and Date: 2011-07-30
• [Presentation] オーキシン輸送体PINを介したシロイヌナズナ胚軸の光屈性 (2011)
  • Author(s): 芳賀健,酒井達也
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 仙台、東北大学
  • Year and Date: 2011-03-11
• [Presentation] 光屈性シグナル伝達因子RPT2の光による発現制御機構の解析 (2011)
  • Author(s): 酒井達也,槌田(間山)智子,上原由紀子,松井南
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 仙台、東北大学
  • Year and Date: 2011-03-11
• [Book] 光屈性におけるオーキシン輸送制御。植物の生長調節 (2012)
  • Author(s): 酒井達也、芳賀健
  • Publisher: 日本植物化学調節学会
• [Book] 光屈性におけるオーキシンシグナリング。植物のシグナル伝達 (2010)
  • Author(s): 酒井達也・柿本辰男・高山誠司・福田裕穂・松岡信編
  • Publisher: 共立出版
• [Remarks]
  • URL: http://wiki.livedoor.jp/tsakai1969/