| Project/Area Number |
22570061
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MORIYAMA Shunsuke 北里大学, 水産学部, 教授 (50222352)
NOZAKI Masumi 新潟大学, 自然科学系, 教授 (70136232)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 比較内分泌学 / 生理学 / 繁殖 / 進化 / ホルモン / 生殖内分泌 / 下垂体 / ヌタウナギ / 遺伝子工学 / 無顎類 / 脊椎動物 |
|---|
| Research Abstract |
To clarify the gonadal functions of most primitive vertebrates, hagfish, we measured plasma concentrations of sex steroid, estradiol, with respect to gonadal development. In both sexes, plasma E2 showed a significant correlation with gonadal development, suggesting that estradiol is involved in the regulation of functional differentiation of gonads. We also isolated the cDNA sequence encoding cholesterol side-chain cleavage enzyme (CYP11A), which involves in the first reaction in the process of steroidogenesis. The enzyme was localized on the Leydig cells and follicle cells in testis and ovary, respectively. The CYP11A transcripts increased significantly in accordance with the developmental condition of gonads. Our findings suggest hagfish might possess similar process of sex steroidogenesis, as proposed in jawed vertebrates.
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