Gonadal functions and their hormonal controls in hagfish

• Project/Area Number: 22570061
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: University of Miyazaki
• Principal Investigator: UCHIDA Katsuhisa 宮崎大学, 農学部, 准教授 (50360508)
• Co-Investigator(Kenkyū-buntansha): MORIYAMA Shunsuke 北里大学, 水産学部, 教授 (50222352) ; NOZAKI Masumi 新潟大学, 自然科学系, 教授 (70136232)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 比較内分泌学 / 生理学 / 繁殖 / 進化 / ホルモン / 生殖内分泌 / 下垂体 / ヌタウナギ / 遺伝子工学 / 無顎類 / 脊椎動物

Research Abstract

To clarify the gonadal functions of most primitive vertebrates, hagfish, we measured plasma concentrations of sex steroid, estradiol, with respect to gonadal development. In both sexes, plasma E2 showed a significant correlation with gonadal development, suggesting that estradiol is involved in the regulation of functional differentiation of gonads. We also isolated the cDNA sequence encoding cholesterol side-chain cleavage enzyme (CYP11A), which involves in the first reaction in the process of steroidogenesis. The enzyme was localized on the Leydig cells and follicle cells in testis and ovary, respectively. The CYP11A transcripts increased significantly in accordance with the developmental condition of gonads. Our findings suggest hagfish might possess similar process of sex steroidogenesis, as proposed in jawed vertebrates.

Research Products

• [Journal Article] Effects of estradiol or testosterone treatment on expression of gonadotropin subunit mRNAs and proteins in the pituitary of juvenile brown hagfish (2013)
  • Author(s): M. Nozaki, K. Uchida, K. Honda, T. Shimotani, M. Nishiyama
  • Journal Title: Paramyxine atami. Gen.Comp.Endocrinol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Glycoprotein hormone in the pituitary of hagfish and its evolutionary implications (2013)
  • Author(s): K. Uchida, S. Moriyama, S. SowerA., M. Nozaki
  • Journal Title: Fish Physiol. Biochem. Volume: 39 Issue: 1 Pages: 75-83
  • DOI: 10.1007/s10695-012-9657-6
  • Peer Reviewed
• [Journal Article] Regulation of growth hormone (GH) receptor (GHR1 and GHR2) mRNA level by GH and metabolic hormones in primary cultured tilapia hepatocytes. (2012)
  • Author(s): A.L. Pierce, J.P. Breves, S. Moriyama, K. Uchida, E.G. Grau
  • Journal Title: Gen. Comp. Endocrinol. Volume: 179 Pages: 22-29
  • Peer Reviewed
• [Journal Article] Chum salmon fry grow faster in seawater, exhibit greater activity of the GH/IGF axis, higher Na+, K+-ATPase activity, and greater gill chloride cell development (2012)
  • Author(s): Iwata M
  • Journal Title: Aquaculture Volume: 362-363 Pages: 101-108
  • DOI: 10.1016/j.aquaculture.2010.10.035
  • Peer Reviewed
• [Journal Article] Induction of sexual maturation of the female honeycomb grouper, Epinephelus merra, in the non-breeding season by modulating environmental factors with GnRH analogue implantation. (2012)
  • Author(s): T. Kanemaru, M. Nakamura, R. Murata, K. Kuroki, H. Horie, K. Uchida, B. Senthilkumaran, H. Kagawa
  • Journal Title: Aquaculture Volume: 358-359 Pages: 85-91
  • DOI: 10.1016/j.aquaculture.2012.06.026
  • Peer Reviewed
• [Journal Article] Body plan of turtles : an anatomical, developmental and evolutionary perspective (2011)
  • Author(s): Nagashima, H., Kuraku, S., Uchida, K., Ohya, K.Y., Narita, Y., Kuratani, S.
  • Journal Title: Anat.Sci.Int. Volume: (in press) Issue: 1 Pages: 1-13
  • DOI: 10.1007/s12565-011-0121-y
  • NAID: 10031162253
  • Peer Reviewed
• [Journal Article] Hagfish, genome duplications, and RFamide neuropeptide evolution (2011)
  • Author(s): T.Osugi, K.Tsutsui, et al
  • Journal Title: Endocrinology Volume: 152 Issue: 11 Pages: 4010-4013
  • DOI: 10.1210/en.2011-1375
  • Peer Reviewed
• [Journal Article] 最も原始的な脊椎動物・ヌタウナギの下垂体から単離された生殖腺刺激ホルモンとその進化的起源 (2011)
  • Author(s): 内田勝久
  • Journal Title: 比較内分泌学 Volume: 37(140) Pages: 32-36
  • NAID: 10027947537
  • URL: https://www.jstage.jst.go.jp/article/nl2008jsce/37/140/37_140_32/_pdf
• [Journal Article] Differential regulation of Igf1 and Igf2 mRNA levels in tilapia hepatocytes : effects of insulin and cortisol on GH sensitivity (2011)
  • Author(s): Pierce, A.L.
  • Journal Title: Journal of Endocrinology Volume: 211 Issue: 2 Pages: 201-210
  • DOI: 10.1530/joe-10-0456
  • Peer Reviewed
• [Journal Article] Evolutionary origin of a functional gonadotropin in the pituitary of the most primitive vertebrate, hagfish. (2010)
  • Author(s): K. Uchida, S. Moriyama, H. Chiba, T. Shimotani, K. Honda, M. Miki, A. Takahashi, S.A. Sower, M. Nozaki
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: 107 Issue: 36 Pages: 15832-15837
  • DOI: 10.1073/pnas.1002208107
  • Peer Reviewed
• [Journal Article] Evolutionary origin of a functional gonadotropin in the pituitary of the most primitive vertebrate, hagfish. (2010)
  • Author(s): K.Uchida, 他8名
  • Journal Title: Proceedings of the National Academy of Sciences of the United State of America 15832-15837. Volume: 107 Pages: 15832-15837
  • Peer Reviewed
• [Presentation] エストロジェン様物質の経口投与によるマコガレイ血中ビテロジェニンの動態 (2013)
  • Author(s): 天野春菜、森山俊介、Yusoff N.B.、宇野誠一、小山次朗
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス, 東京
• [Presentation] クロヌタウナギにおけるコレステロール側鎖切断酵素のクローニングと発現解析 (2012)
  • Author(s): 西山真樹、内田勝久、下谷豊和、野崎眞澄
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪大学豊中キャンパス,大阪
• [Presentation] Study on sex steroid hormones in the hagfish-Their serum concentrations and sex steroid biosynthetic enzymes. (2012)
  • Author(s): M. Nishiyama, K. Uchida, S. Moriyama, H. Chiba, S. Awata, M. Nozaki
  • Organizer: 7th Asia and Oceania Society for Comparative Endocrinology Congress
  • Place of Presentation: Kuala Lumpur, Malaysia
• [Presentation] Evolutionary origin of a functional gonadotropin in the pituitary of the most primitive vertebrate, hagfish. (2012)
  • Author(s): M. Nozaki, T. Shimotani, M. Nishiyama, K. Uchida
  • Organizer: 7th Asia and Oceania Society for Comparative Endocrinology Congress
  • Place of Presentation: Kuala Lumpur, Malaysia
• [Presentation] クロヌタウナギにおけるコレステロール側鎖切断酵素のクローニングと発現動態 (2012)
  • Author(s): 西山真樹、内田勝久、下谷豊和、野崎眞澄
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪大学豊中キャンパス
• [Presentation] Study on sex steroid hormones in the hagfish-Their serum concentrations and sex steroid biosynthetic enzymes (2012)
  • Author(s): Nishiyama M., Uchida K., Moriyama S., Chiba H., Awata S., Nozaki M.
  • Organizer: 7th Asia and Oceania Society for Comparative Endocrinology Congress
  • Place of Presentation: Kuala Lumpur, Malaysia
• [Presentation] Evolutionary origin of a functional gonadotropin in the pituitary of the most primitive vertebrate, hagfish (2012)
  • Author(s): Nozaki, M., Shimotani, T., Nishiyama, M., Uchida, K.
  • Organizer: 7th Asia and Oceania Society for Comparative Endocrinology Congress
  • Place of Presentation: Kuala Lumpur, Malaysia
• [Presentation] 原始脊椎動物・ヌタウナギ類の糖タンパク質ホルモンとその進化. (2011)
  • Author(s): 内田勝久、森山俊介、野崎眞澄(他3 名).
  • Organizer: 日本比較内分泌学会第35回静岡大会
  • Place of Presentation: 静岡グランシップ, 静岡
  • Year and Date: 2011-11-19
• [Presentation] クロヌタウナギにおける性ステロイド合成酵素の探索 (2011)
  • Author(s): 西山真樹、内田勝久、森山俊介、千葉洋明、下谷豊和、野崎眞澄
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 北海道旭川市
• [Presentation] Evolutionary origin of a functional gonadotropin in the pituitary of the most primitive vertebrate, hagfish. (2011)
  • Author(s): K. Uchida, S. Moriyama, S.A. Sower, M. Nozaki
  • Organizer: 9th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: Cochin, India
• [Presentation] Evolutionary origin of a functional gonadotropin in the pituitary of the most primitive vertebrate, hagfish (2011)
  • Author(s): Uchida K., Moriyama S., Sower S.A.Nozaki M.
  • Organizer: 9th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: Cochin, India(招待講演)
• [Presentation] クロヌタウナギの生殖腺における性ステロイド産生能の探索. (2010)
  • Author(s): 西山真樹、内田勝久、森山俊介、野崎眞澄(他2 名)
  • Organizer: クロヌタウナギの生殖腺における性ステロイド産生能の探索
  • Place of Presentation: 静岡グランシップ, 静岡
  • Year and Date: 2010-11-19
• [Presentation] クロヌタウナギの生殖腺における性ステロイド産生能の探索 (2010)
  • Author(s): 西山真樹, 他5名
  • Organizer: 日本比較内分泌学会 第35回静岡大会
  • Place of Presentation: 静岡グランシップ(静岡)
  • Year and Date: 2010-11-19
• [Presentation] 原始脊椎動物・ヌタウナギ類の糖タンパク質ホルモンとその進化 (2010)
  • Author(s): 内田勝久, 他5名
  • Organizer: 日本比較内分泌学会 第35回静岡大会
  • Place of Presentation: 静岡グランシップ(静岡)
  • Year and Date: 2010-11-09
• [Presentation] クロヌタウナギの下垂体から精製した糖タンパク質ホルモンの生理活性 (2010)
  • Author(s): 内田勝久、亀井宏康、森山俊介、野崎眞澄(他2 名)
  • Organizer: 日本動物学会第81回東京大会
  • Place of Presentation: 東京大学教養部, 東京
  • Year and Date: 2010-09-23
• [Presentation] ヌタウナギ下垂体のGTH 細胞に対するエストロゲンとテストステロン投与の影響 (2010)
  • Author(s): 野崎眞澄、本田香織、三木誠、下谷豊和、内田勝久
  • Organizer: 日本動物学会第81回東京大会
  • Place of Presentation: 東京大学教養部, 東京
  • Year and Date: 2010-09-23
• [Presentation] クロヌタウナギの下垂体から精製した糖タンパク質ホルモンの生理活性 (2010)
  • Author(s): 内田勝久, 他4名
  • Organizer: 日本動物学会 第81回東京大会
  • Place of Presentation: 東京大学教養部(東京都)
  • Year and Date: 2010-09-23
• [Presentation] ヌタウナギ下垂体のGTH細胞に対するエストロゲンとテストステロン投与の影響 (2010)
  • Author(s): 野崎眞澄, 他3名
  • Organizer: 日本動物学会 第81回東京大会
  • Place of Presentation: 東京大学教養部(東京都)
  • Year and Date: 2010-09-23
• [Book] Prolactin (G.M. Nagy, B.E.Toth, eds) (2013)
  • Author(s): H. Takahashi, H. Kudose, C. Takagi, S. Moriyama, T. Sakamoto
  • Publisher: In Tech
• [Remarks] 新潟大学ホームページ
  • URL: http://www.niigata-u.ac.jp/research/10_research_010/hagfish.html