Epigenetics during pollination and fertilization in higher plants

• Project/Area Number: 22570068
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Yokohama City University
• Principal Investigator: TANAKA Ichiro 横浜市立大学, 大学院・生命ナノシステム科学研究科, 教授 (60175445)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 精細胞 / 花粉 / 花粉管 / ヒストン / 雄原細胞 / 雄性配偶子 / 遺伝子発現制御 / small RNA / ヒストン修飾

Research Abstract

In this study, we first showed that Argonaute (AGO) was specifically expressed in the generative and sperm cells in Lilium longiflorum. This suggests that the gene expression in male gametic cells might be regulated epigenetically. Futher, we found dimorphism between two sperm cells in the nuclear chromatin

Research Products

• [Journal Article] Expression of foreign aquaporin genes in lily pollen protoplasts (2011)
  • Author(s): Yamada,K., Yanada,K., Matsuzawa,A., Tanaka,I., Shiota,H.
  • Journal Title: Plant Biotechnology Volume: 28 Issue: 5 Pages: 509-514
  • DOI: 10.5511/plantbiotechnology.11.0922a
  • NAID: 10030304178
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed
• [Journal Article] Molecular morphological studies on pollen development using protoplasts (2011)
  • Author(s): Tanaka,I
  • Journal Title: Plant Morphol Volume: 23 Pages: 53-59
  • NAID: 130002152899
  • URL: http://square.umin.ac.jp/pl-morph/
  • Peer Reviewed
• [Journal Article] プロトプラストを用いた花粉発生の分子形態学 (2011)
  • Author(s): 田中一朗
  • Journal Title: Plant Morphology Volume: 23 Pages: 53-59
  • NAID: 130002152899
• [Journal Article] Characterization of bacterial-type phosphoenolpyruvate carboxylase expressed in male gametophyte of higher plants (2010)
  • Author(s): Igawa,T., Fujiwara,M., Tanaka,I.,Fukao,Y., Yanagawa,Y
  • Journal Title: BMC Plant Biology Volume: 10 Issue: 1 Pages: 200-211
  • DOI: 10.1186/1471-2229-10-200
  • Peer Reviewed
• [Journal Article] Nucleotide polymorphism in the carrot embryogenesis abundant geneDC8/ECP63 (2010)
  • Author(s): Shiota,H., Matsuzawa,A., Kurokawa,K., Sugimoto,G., Yonezawa,M., Tanaka,I.,kamada,H
  • Journal Title: Scientia Horticulturae Volume: 125 Issue: 4 Pages: 767-770
  • DOI: 10.1016/j.scienta.2010.05.001
  • Peer Reviewed
• [Journal Article] 花粉内栄養細胞の分化とクロマチン (2010)
  • Author(s): 田中一朗、最上則史、佐野弥生子
  • Journal Title: 横浜市立大学論叢自然科学系列 Volume: 60 Issue: 3 Pages: 41-49
  • DOI: 10.1042/cbi20090124
  • NAID: 120005681984
• [Journal Article] Distinct localization of histone H3 methylation in the vegetative nucleus of lily Pollen. (2010)
  • Author(s): Sano, Y., Tanaka, I.
  • Journal Title: Cell Biology International Volume: 34 Pages: 253-259
  • Peer Reviewed
• [Journal Article] Nucleotide polymorphism in the carrot embryogenesis abundant gene DC8/ECP63. (2010)
  • Author(s): Shiota, H., Matsuzawa, A., Kurokawa, K., Sugimoto, G., Yonezawa, M., Tanaka, I., Kamada, H.
  • Journal Title: Scientia Horticulturae Volume: 125 Pages: 767-770
  • Peer Reviewed
• [Journal Article] Chracterization of bacterial-type phosphoenolpyruvate carboxylase expressed in male gametophyte of higher plants. (2010)
  • Author(s): Igawa, T., Fujiwara, M., Tanaka.I., Fukao, Y., Yanagawa, Y.
  • Journal Title: BMC Plant Biology Volume: 10 Pages: 200-211
  • Peer Reviewed
• [Presentation] 朝倉さくら子、ニンジン液胞膜アクアポリン遺伝子 DcTIP3 の発現解析 (2012)
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-16
• [Presentation] ニンジン液胞膜アクアポリン遺伝子DcTIP3の発現解析 (2012)
  • Author(s): 朝倉さくら子、松澤篤史、田中元気、菊池彰、鎌田博、田中一朗、塩田肇
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-16
• [Presentation] テッポウユリ花粉に特異的なArgonaute(AGO)の発現解析 (2011)
  • Author(s): 新屋智尋
  • Organizer: 第29回日本植物細胞分子生物学会
  • Place of Presentation: 九州大学
  • Year and Date: 2011-09-07
• [Presentation] 細胞からみる花粉の使命 (2010)
  • Author(s): 田中一朗
  • Organizer: 本花粉学会第51回大会
  • Place of Presentation: 中央大学
  • Year and Date: 2010-10-09
• [Presentation] 細胞からみる花粉の使命 (2010)
  • Author(s): 田中一朗
  • Organizer: 日本花粉学会第51回大会
  • Place of Presentation: 中央大学(招待講演)
  • Year and Date: 2010-10-09
• [Presentation] テッポウユリの花粉管伸長過程における栄養核クロマチンの変化 (2010)
  • Author(s): 佐野弥生子
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学
  • Year and Date: 2010-09-11
• [Presentation] テッポウユリ花粉におけるArgonaute 様遺伝子の単離と発現解析 (2010)
  • Author(s): 新屋智尋
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学
  • Year and Date: 2010-09-11
• [Presentation] テッポウユリ花粉におけるArgonaute様遺伝子の単離と発現解析 (2010)
  • Author(s): 新屋智尋、岩田真也、最上則史、田中一朗
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学
  • Year and Date: 2010-09-11
• [Presentation] プロトプラストを用いた花粉発生の分子形態学 (2010)
  • Author(s): 田中一朗
  • Organizer: 日本植物形態学会第22回大会
  • Place of Presentation: 中部大学
  • Year and Date: 2010-09-08
• [Presentation] プロトプラストを用いた花粉発生の分子形態学 (2010)
  • Author(s): 田中一朗
  • Organizer: 日本植物形態学会第22回大会
  • Place of Presentation: 中部大学(招待講演)
  • Year and Date: 2010-09-08
• [Presentation] Expression and functional analysis of two kinds of the VP1/ABI3 factors in carrot embryogenesis. (2010)
  • Author(s): Noudomi, K., Tanaka, L, Kamada, H., Shiota, H.
  • Organizer: 21^<st> International Conference on Arabidopsis Research
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2010-06-08
• [Presentation] Expression analysis of the aquaporin genes in carrot somatic embryogenesis. (2010)
  • Author(s): Asakura, S., Matsuzawa, A., Tanaka, L., Kamada, H., Shiota, H.
  • Organizer: 21^<st> International Conference on Arabidopsis Research
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2010-06-08
• [Presentation] Lily pollen protoplast system for functional analysis of plant Aquaporins. (2010)
  • Author(s): Yanada, K., Yamada, K, Matsuzawa, A., Tanaka, I., Shiota, H.
  • Organizer: 21^<st> International Conference on Arabidopsis Research
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2010-06-08
• [Presentation] Carrot embryogenesis for analysis of desiccation tolerance and abscisicacid signal transduction. (2010)
  • Author(s): Shiota, H., Ko, S., Takatsuka, M., Tanaka., Kamada, H.
  • Organizer: 21^<st> International Conference on Arabidopsis Research
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2010-06-07
• [Book] 花粉の世界をのぞいてみたら (2012)
  • Author(s): 田中一朗
  • Total Pages: 335
  • Publisher: エヌ・ティー・エス
• [Book] 花粉の世界をのぞいてみたら (2012)
  • Author(s): 医学生物学電子顕微鏡技術学会
  • Total Pages: 335
  • Publisher: エヌ・ティー・エス
• [Book] 生物学辞典 (2010)
  • Author(s): 田中一朗
  • Total Pages: 1620
  • Publisher: 東京化学同人
• [Book] 生物学辞典 (2010)
  • Author(s): 田中一朗(分担)
  • Publisher: 東京化学同人
• [Remarks]
  • URL: http://histone.sci.yokohama-cu.ac.jp/
• [Remarks]