| Project/Area Number |
22570076
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SHIGA Sakiko 大阪市立大学, 理学(系)研究科(研究院), 教授 (90254383)
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUMURA Ryusei 川崎医科大学, 医学部, 助教 (90388934)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 羽化リズム / ハエ目昆虫 / 概日時計 / ペプチドニューロン / 免疫電顕法 / キイロショウジョウバエ / シリアカニクバエ / 免疫組織化学 / PDF / CCAP |
|---|
| Research Abstract |
To understand how the circadian clocks time the gate of eclosion in Drosophila melanogaster, we examined the neuronal connection focusing on the putative clock neurons, PDF-Tri neurons, that transiently occur associated with eclosion, and showed the synaptic connections between the PDF-Tri and the peptidergic (CCAP-, EH- or corazonin) neurons involved in eclosion behavior. We also analyzed the eclosion rhythms in the flesh fly Sarcophaga crassipalpis, and revealed that two mechanisms are required for determination of the gate of eclosion. One is driven by low titer of 20-hydroxyecdysone between 20 h and 16 h before eclosion, and the other is driven by the signal from the circadian clock transmitted later than 6 h before eclosion.
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