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Molecular cell biological studies on the evolution of male and female sex in algae.

Research Project

Project/Area Number 22570084
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biodiversity/Systematics
Research InstitutionUniversity of Tsukuba

Principal Investigator

MIYAMURA Shinichi  筑波大学, 生命環境系, 准教授 (00192766)

Co-Investigator(Kenkyū-buntansha) KAWANO Shigeyuki  東京大学, 新領域創成科学研究, 教授 (70161338)
MOTOMURA Taizo  北海道大学, 北方生物圏フィールド科学センター, 教授 (30183974)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords性 / 配偶子 / 雌雄性 / 藻類 / 受精 / 海産緑藻 / 接合装置 / 雌雄 / 細胞融合 / 緑藻
Research Abstract

Spatial arrangement of gamete fusion site or mating structure was studied in ulvophycean green algae, Chaetomorpha spiralis, Monostroma angicava and chlorophycean green alga Gonium pectoral using electron microsco py. In all species, cell fusion site of the gamete was located on the same side of the eyespot in one sex type while it was located on the other side of the eyespot in the opposite mating type. These results support the proposal that sex specific arrangement of cell fusion site or mating structure may be a universal feature in chlorophyte algae. The relationship between the sex determination gene MID and sex specific arrangement of mating structure was studied using G. pectorale. In the MID+ gametes, mating structure was present on the same side as the eyespot while in the MID- gametes mating structure was present on the opposite side of the eyespot. These results are consistent with that of Chlamydmonas reinhardtii and suggest that the close relationship between presence or absence of MID and sex specific arrangement of mating structure in these algae.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (34 results)

All 2013 2012 2011 2010

All Journal Article (21 results) (of which Peer Reviewed: 20 results) Presentation (13 results)

  • [Journal Article] Localization and evolution of septins in algae2013

    • Author(s)
      Yamazaki, T., Owari, S., Ota, S., Sumiya, N., Yamamoto, M., Watanabe, K., Nagumo, T., Miyamura, S. and Kawano, S
    • Journal Title

      Plant J

      Volume: (in press) Issue: 4 Pages: 12147-8

    • DOI

      10.1111/tpj.12147

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Three dimensional ultrastructural study of oil and astaxanthin accumulation during encystment in the green alga Haematococcus pluvialis2013

    • Author(s)
      Wayama, M., Ota, O., Matsuura, M., Nango, N., Hirata, A., and Kawano, S
    • Journal Title

      PLoS ONE

      Volume: 8(1) Issue: 1 Pages: 53618-9

    • DOI

      10.1371/journal.pone.0053618

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sex specific sapatial arrangement of gamete fusion site in chlorophyte algae.2012

    • Author(s)
      Miyamura, S.
    • Journal Title

      Aquabiology

      Volume: 34 Pages: 443-452

    • URL

      http://aquabiology.blog93.fc2.com/category7-1.html

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] Ultrastructural Study ofplasmodesmata in the brown Alga Dictyota dichotoma (Dictyotales, Phaeophyceae)2012

    • Author(s)
      Terauchi, M., Nagasato, C., Kajimura, N., Mineyuki, Y., Okuda, K., Katsaros, C., Motomura. T
    • Journal Title

      Planta

      Volume: 236 Issue: 4 Pages: 1013-1026

    • DOI

      10.1007/s00425-012-1656-4

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ultrastructural analysis of flagellar development in plurilocular sporangia of Ectocarpus siliculosus (Phaeophyceae).2012

    • Author(s)
      Fu, G., Nagasato, C., Ito, T., Muller, D. G., Motomura, T.
    • Journal Title

      Protoplasma

      Volume: 205 Issue: 1 Pages: 261-272

    • DOI

      10.1007/s00709-012-0405-7

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Three dimensional ultrastructural study of oil and astaxanthin accumulation during encystment in the green alga Haematococcus.2012

    • Author(s)
      Wayama, M., Ota, S., Matuura,H., Nango, N., Hirata, A., and Kawano, S.
    • Journal Title

      PLOS One

      Volume: 8

    • URL

      http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053618

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The role of multiple FtsZ rings in chloroplast division underoligotrophic and eutrophic conditions in the unicellular green alga, Nannochloris bacillaris (Chlorophyta, Trebouxiophyceae)2012

    • Author(s)
      Sumiya, N., Owari, S., Watanabe, K., and Kawano, S
    • Journal Title

      J. Phycol

      Volume: 48 Issue: 5 Pages: 1187-1196

    • DOI

      10.1111/j.1529-8817.2012.01204.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of mitochondrial and plastid ftsZ genes and analysis 1 of the organelle targeting sequence in the diatom Chaetoceros neogracile (Diatoms,Bacillariophyceae)2012

    • Author(s)
      Nishikawa, T., Moriyama, Y., Sato, M., Sano, T., Hasezawa, S., Ota, S., and Kawano, S
    • Journal Title

      Phycol. Res

      Volume: 60 Issue: 2 Pages: 123-136

    • DOI

      10.1111/j.1440-1835.2012.00644.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chloroplast division and differentially regulated expression of FtsZ1 and FtsZ2 in Nannochloris bacillalis (Chlorophyta, Trebouxiophyceae)2012

    • Author(s)
      Sumiya, N., Watanabe, K., Owari, S., Yamamot, M., and Kawano, S.
    • Journal Title

      Cytologia

      Volume: 77 Pages: 59-66

    • URL

      https://www.jstage.jst.go.jp/article/cytologia/77/1/77_59/_pdf

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ultrastructural analysis of flagellar development in plurilocular sporangia of Ectocarpus siliculosus (Phaeophyceae).2012

    • Author(s)
      Fu, G.
    • Journal Title

      Protoplasma

      Volume: 205 Pages: 261-272

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA Content of Stichococcus bacillaris(Trebouxiophyceae, Chlorophyta) nuclei determined with laser scanning cytometry.2011

    • Author(s)
      Yamamoto, M., Handa, S. and Kawano, S.
    • Journal Title

      Cytologia

      Volume: 76 Pages: 157-161

    • NAID

      10029478433

    • URL

      https://www.jstage.jst.go.jp/article/cytologia/76/2/76_2_157/_pdf

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Duration of its cell cycle and phases at growth rates affected by light in tensrty.2011

    • Author(s)
      Vitova, M.
    • Journal Title

      Planta

      Volume: 233 Pages: 75-86

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparative immunofluorescence and ultrastructural analysis of microtubule organization in Uronema sp., Klebsormidium flaccidum, K. subtilissimum, Stichococcus bacillaris and S. chloranthus (Chlorophyta)2010

    • Author(s)
      Katsaros, C, Varvarigos, V., Gachon, C.M. M., Brand, J., Motomura, T., Nagasato, C., Kupper, F. C
    • Journal Title

      Protist

      Volume: 162 Issue: 2 Pages: 315-331

    • DOI

      10.1016/j.protis.2010.10.004

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of chloroplast FtsZ and AtpC, and analysis of protein targeting into the complex chloroplast of the haptophyte Pavlova pinguis.2010

    • Author(s)
      Nishikawa, T., Kajitani, H., Sato, M., Mogi, Y., Moriyama, Y. and Kawano, S.Nishikawa, T., Kajitani, H., Sato, M., Mogi, Y., Moriyama, Y. and Kawano, S.
    • Journal Title

      Cytologia

      Volume: 75 Pages: 203-210

    • NAID

      10026984048

    • URL

      https://www.jstage.jst.go.jp/FF01S040Init?sourceurl=%2Farticle%2Fcytologia%2F75%2F2%2F75_2_203%2F_pdf

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Membrane fusion process and assembly of cell wall during cytokinesis in the brown alga, Silvetia babingtonii (Fucales, Phaeophyceae)2010

    • Author(s)
      Nagasato, C., Inoue, A., Mizuno, M., Kanazawa, K. Ojima, T., Okuda, K., Motomrua, T
    • Journal Title

      Planta

      Volume: 232 Issue: 2 Pages: 287-298

    • DOI

      10.1007/s00425-010-1188-8

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of expressed sequence tags from the chrysophycean alga Ochromonas danica (Heterokontophyta)2010

    • Author(s)
      Terauchi, M., Kato, A., Nagasato, C. and Motomura, T.
    • Journal Title

      Phycological Research

      Volume: 58 Issue: 3 Pages: 217-221

    • DOI

      10.1111/j.1440-1835.2010.00578.x

    • NAID

      10027507157

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cytoplasmic inheritance in green algae: patterns, mechanisms and relation to sex type.2010

    • Author(s)
      Miyamura, S.
    • Journal Title

      J. Plant Res.

      Volume: 123 Issue: 2 Pages: 171-184

    • DOI

      10.1007/s10265-010-0309-6

    • NAID

      10027218095

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Behavior of flagella and flagellar root systems in the planozygotes and settled zygotes of the green alga Bryopsis maxima Okamura (Ulvophyceae, Chlorophyta) with reference to spatial arrangement of eyespot and cell fusion site.2010

    • Author(s)
      Miyamura, S., Sakaushi, S., Horo, T. and Nagumo, T.
    • Journal Title

      Phycol. Res.

      Volume: 58 Issue: 4 Pages: 258-269

    • DOI

      10.1111/j.1440-1835.2010.00585.x

    • NAID

      10026703935

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cytoplasmic inheritanoe in green algae : patterns, mechanisms and their Prelations with sex types2010

    • Author(s)
      宮村新一
    • Journal Title

      J Plant Research

      Volume: 123 Pages: 171-184

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Behavior of flagellar root systems in the planozygotes and settled zygotes of the green alga Bryopsis maxima (Ulvophyceae, Chlorophyta) with reference to spatial arrangement of eyespot and cell fusion site.2010

    • Author(s)
      宮村新一
    • Journal Title

      Phycological Research

      Volume: 58 Pages: 256-269

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of chloroplast FtsZ and AtpC, and analysis of protein targeting into the complex chloroplast of the haptophyte Pavlova pinguis2010

    • Author(s)
      Nishikawa, T
    • Journal Title

      Cytologia

      Volume: 75 Pages: 203-210

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 緑藻Gonium pectorale の配偶子における接合装置の空間配置と交配型の関係2012

    • Author(s)
      梅澤 歩、横田真吾、宮村新一、南雲 保、河野重行、浜地貴志、野崎久義
    • Organizer
      日本藻類学会第36回大会
    • Place of Presentation
      札幌、北海道大学
    • Related Report
      2012 Final Research Report
  • [Presentation] 緑藻Gonium pectorale の配偶子における接合装置の空間配置と交配型の関係2012

    • Author(s)
      宮村新一
    • Organizer
      日本藻類学会第36回大会
    • Place of Presentation
      札幌, 北海道大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] The filament and colony formation caused by deficiency of cleavage and dissociation of the mother cell wall in Trebouxiophyceae2011

    • Author(s)
      山本真紀
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      Yeosu, Korea
    • Year and Date
      2011-10-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] イチョウ精子の構造と進化2011

    • Author(s)
      宮村新一
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      旭川 大雪クリスタルホール
    • Year and Date
      2011-09-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] 緑藻Gonium pectoraleの同形配偶子における細胞融合部位の性特異性2011

    • Author(s)
      宮村新一
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京大学駒場キャンパス
    • Year and Date
      2011-09-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] トレボキシア藻の娘細胞連結による糸状化と細胞塊形成2011

    • Author(s)
      山本真紀
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京大学駒場キャンパス
    • Year and Date
      2011-09-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] カニクサのジベレリン応答関連遺伝子の構造解析2011

    • Author(s)
      山内大輔
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京大学駒場キャンパス
    • Year and Date
      2011-09-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] イチョウ精子の構造と進化2011

    • Author(s)
      宮村新一
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      旭川.旭川市神楽地区公共施設群
    • Related Report
      2012 Final Research Report
  • [Presentation] 緑藻Gonium pectorale の同形配偶子における細胞融合部位の性特異性2011

    • Author(s)
      横田真吾、宮村新一. 南雲 保
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京.東京大学
    • Related Report
      2012 Final Research Report
  • [Presentation] 海産緑藻オオハネモの接合子における雌雄鞭毛装置と眼点の挙動2010

    • Author(s)
      宮村新一
    • Organizer
      に本職物学会第74回大会
    • Place of Presentation
      中部大学
    • Year and Date
      2010-09-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] 母細胞壁の開裂と脱離の有無から見たトレボキシア藻の娘細胞連結2010

    • Author(s)
      山本真紀
    • Organizer
      に本職物学会第74回大会
    • Place of Presentation
      中部大学
    • Year and Date
      2010-09-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] 海産緑藻オオハネモの接合における雌雄鞭毛装置と眼点の挙動2010

    • Author(s)
      宮村新一、南雲 保
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      春日井市、中部大学
    • Related Report
      2012 Final Research Report
  • [Presentation] 母細胞壁の界列と脱離の有無からみたトレボキシア藻の娘細胞連結2010

    • Author(s)
      山本真紀、半田信司、宮村新一、南 雲 保、河野重行
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      春日井市、中部大学
    • Related Report
      2012 Final Research Report

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Published: 2010-11-30   Modified: 2019-07-29  

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