| Project/Area Number |
22570105
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | Higashichikushi Junior College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAGANO Yuriko 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 研究員 (30512917)
MIYAZAKI Masayuki 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 技術研究副主事 (50399573)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 菌類 / LKM11 クレード / 初期進化 / LKM11クレード |
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| Research Abstract |
For exploring early evolution of fungi, we investigated fungaldiversity in sediments colleted in eight lakes in west Japan. By using culture-independentmethod with fungal-specific PCR of SSU rDNA, we revealed an large extent of novel fungaldiversity in lacustrine environment. Four hundreds fungal phylotypes were obtained fromPCR clone libraries of six lakes successfully amplified. Novel (<97% identity) phylotypesare 213 (53.3%) in all fungal phylotypes. There were 187 phylotypes (46.8%) related toDikarya (Ascomycota + Basidiomycota). Whereas, Non-Dikarya fungi (Chytridiomycota +Blastocladiomycota + LKM11 etc) were 53.3%, 74.8% of which were estimated to be novelphylotypes with <97% affinities. All LKM11 phylotypes were novel. Early fungal lineagesoccupied a majority of all fungal phylotypes from the lacustrine environment in west Japanand indicated an unexpected diversity and novelty.
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