Elucidation of the mechanism of receptor activation by ENF peptide family
| Project/Area Number |
22570108
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
KAWANO Keiichi 北海道大学, 大学院・先端生命科学研究院, 特任教授 (10136492)
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| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | サイトカイン / 昆虫 / 自然免疫 / NMR |
|---|
| Research Abstract |
ENF peptides are insect cytokines that possesses diverse biological activities such as larval growth regulation, cell proliferation, and stimulation of immune cells. In the present study, we constructed the expression systems of ENF peptides using the methylotrophic yeast Pichia pastoris, which is an efficient host for high-level production of heterologous proteins. To inhibit degradation by protease, we developed the method to produce peptides fused to thioredoxin and we successfully produced ENF peptides efficiently. In addition, we studied the interactions between ENF peptides and GBP-BP that is related to the end of the activation of ENF peptides.
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Report
(4 results)
Research Products
(10 results)
