Development of NMR measurement and analysis method for protein structural study in 2H2O solution.
| Project/Area Number |
22570109
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
OGURA Kenji 北海道大学, 大学院・先端生命科学研究院, 特任准教授 (50270682)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | NMR / タンパク質 / 立体構造解析 |
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| Research Abstract |
We developed an NMR pulse sequence, 3D HCA(N)CO, to correlate the chemical shifts of protein backbone 1H and 13C to those of 13C' in the preceding residue. When combined with HCACO, HCAN and HCA(CO)N, the HCA(N)CO sequence allows the sequential assignment using backbone 13C' and amide 15N chemical shifts without resort to backbone amide protons. This assignment strategy was demonstrated for 13C/15N-labeled GB1 dissolved in 2H2O. The quality of the GB1 structure determined in 2H2O was similar to that determined in H2O in spite of significantly smaller number of NOE correlations. Thus this strategy enables the determination of protein structures in 2H2O or H2O at high pH values.
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Report
(4 results)
Research Products
(45 results)
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[Presentation] 酵母カルモジュリンの特異な標的認識機構2010
Author(s)
小椋賢治,久米田博之,高橋清大,小橋川敬博,吉田良輔,伊藤浩之,矢澤道生,稲垣冬彦
Organizer
第10回日本蛋白質科学会年会
Place of Presentation
札幌コンベンションセンター(札幌市)
Year and Date
2010-06-16
Related Report
