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Functional analysis of fatty acid-binding proteins (FABPs) involved in nuclear receptor signaling

Research Project

Project/Area Number 22570112
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionShinshu University

Principal Investigator

FUJII Hiroshi  信州大学, 農学部, 教授 (90165340)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords核内受容体 / 脂肪酸結合タンパク質 / 脂肪酸 / 胆汁酸 / シグナル伝達 / 遺伝子発現 / 癌転移 / miRNA / マイクロRNA
Research Abstract

(1) Functional analysis of FABP5 in nuclear receptor PPAR signaling in carcinogenesis or metastasis: I have found that nuclear receptor PPAR target gene, FABP5, is up-regulated in human malignant prostate cancer cellsand that is localized into the nucleus in a ligand (fatty acid)-dependent manner.
(2)Functional analysis of FABP6 in nuclear receptor FXR signaling: I have identified novel miRNAs and their target genes that were activated by the FXR agonist. Furthermore, I have demonstrated that a variant protein for FABP6, called FABP6-L/IBABP-L, has exhibited anti-apoptotic activity in the presence of bile acid in colorectal cancer cells.
(3) Activation mechanisms of FABP5 gene expression: I have found that DNA methylation status of FABP5 gene isolated from PC-3 is much more demethylated compared withthat of the gene from PNT-2.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (40 results)

All 2013 2012 2011 2010 Other

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (26 results)

  • [Journal Article] Makabe Syntheses of prodelphinidin B3 and C2, and their antitumor activities through cell cycle arrest and caspase-3 activation.2013

    • Author(s)
      W. Fujii, K. Toda, K. Kawaguchi, S. Kawahara, M. Katoh, Y. Hattori, H. Fujii, H.
    • Journal Title

      Tetrahedron

      Volume: 69 Pages: 3543-3550

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Syntheses of prodelphinidin B3 and C2, and their antitumor activities through cell cycle arrest and caspase-3 activation.2013

    • Author(s)
      W. Fujii, K. Toda, K. Kawaguchi, S. Kawahara, M. Katoh, Y. Hattori, H. Fujii, H. Makabe
    • Journal Title

      Tetrahedron

      Volume: 69 Pages: 3543-3550

    • NAID

      120007106201

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Makabe Synthesis of procyanidin C2 & C1 using Lewis acid mediated equimolar condensation.2012

    • Author(s)
      Y. Oizumi, M. Katoh, Y. Hattori, K. Toda, K. Kawaguchi, H. Fujii, and H.
    • Journal Title

      Heterocycles

      Volume: 85 (9) Pages: 2241-2250

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of procyanidin C2 & C1 using Lewis acid mediated equimolar condensation.2012

    • Author(s)
      Y. Oizumi, M. Katoh, Y. Hattori, K. Toda, K. Kawaguchi, H. Fujii, and H. Makabe
    • Journal Title

      Heterocycles

      Volume: 85 Pages: 2241-2250

    • NAID

      120007106196

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hatakeyama Diagnosis of ischemic small bowel disease by measurement of serum intestinal fatty acid-binding protein in patients with acute abdomen: a multicenter, observer-blinded validation study.2011

    • Author(s)
      T. Kanda, A. Tsukahara, K. Ueki, Y. Sakai, T. Tani, A. Nishimura, T. Yamazaki, Y. Tamiya, T. Tada, M. Hirota, J. Hasegawa, H. Funaoka, H. Fujii, K.
    • Journal Title

      J. Gastroenterology

      Volume: 46 Pages: 492-500

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a high-specificity sandwich ELISA system for the quantificaion of human intestinal fatty acid-binding protein (I-FABP) concentrations.2011

    • Author(s)
      H. Funaoka, T. Kanda, S. Kajiura, Y. Ohkaru, and H. Fujii
    • Journal Title

      Immunological Investigation

      Volume: 40(1) Pages: 1-20

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of light wavelength and intensity on the expression of photoresponse genes in oyster mushroom mycelia.2011

    • Author(s)
      Y. Nakano, H. Fujii, M. Kojima
    • Journal Title

      J. Light & Visual Environment

      Volume: 35 (1) Pages: 90-94

    • NAID

      10028106653

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Diagnosis of ischemic small bowel disease by measurement of serum intestinal fatty acid-binding protein in patients with acute abdomen : a multicenter, observer-blinded validation study2011

    • Author(s)
      T.Kanda, A.Tsukahara, K.Ueki, Y.Sakai, T.Tani, A.Nishimura, T.Yamazaki, Y.Tamiya, T.Tada, M.Hirota, J.Hasegawa, H.Funaoka, H.Fujii, K.Hatakeyama
    • Journal Title

      J.Gastroenterol.

      Volume: 46 Issue: 4 Pages: 492-500

    • DOI

      10.1007/s00535-011-0373-2

    • NAID

      10029028597

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of light wavelength and intensity on the expression of photoresponse genes in oyster mushroom mycelia2011

    • Author(s)
      Y.Nakano, H.Fujii, M.Kojima
    • Journal Title

      J.Light & Visual Environment

      Volume: 35 Pages: 90-94

    • NAID

      10028106653

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a high-specificity sandwich ELISA system for the quantificaion of human intestinal fatty acid-binding protein(I-FABP)concentrations.2011

    • Author(s)
      H.Funaoka, TKanda, S.Kajiura, Y.Ohkaru, H.Fujii
    • Journal Title

      Immunological Investigation

      Volume: 40 Pages: 1-20

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of blue-light photoresponse genes in oyster mushroom mycelia2010

    • Author(s)
      Y. Nakano, H. Fujii, M. Kojima
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 74 Pages: 2160-2165

    • NAID

      10027561046

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Light stimulation triggered expression of gene coding for vacuolar proton-pump enzymes v-ATPase and v-PPase in buckwheat.2010

    • Author(s)
      M. Kojima, Y. Nakano, H. Fujii
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 74 (7) Pages: 1507-1511

    • NAID

      10027557708

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of blue-light photoresponse genes in oyster mushroom mycelia.2010

    • Author(s)
      Y.Nakano, H.Fujii, M.Kojima
    • Journal Title

      Biosci.Biotechnol.Biochem.

      Volume: 74 Pages: 2160-2165

    • NAID

      10027561046

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Light stimulation triggered expression of gene coding for vacuolar proton-pump enzymes v-ATPase and v-PPase in buck wheat.2010

    • Author(s)
      M.Kojima, Y.Nakano, H.Fujii
    • Journal Title

      Biosci.Biotechnol.Biochem.

      Volume: 74 Pages: 1507-1511

    • NAID

      10027557708

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] ヒト前立腺癌細胞における転移原因遺伝子FABP5の発現制御機構の解析2012

    • Author(s)
      川口 耕一郎、木滑 歩、鈴木 俊介、藤井 博
    • Organizer
      第85回日本生化学会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-15
    • Related Report
      2012 Final Research Report
  • [Presentation] 転移原因遺伝子FABP5を分子標的とした抗腫瘍性分子の同定と作用機構の解2012

    • Author(s)
      戸田 一弥, 川口 耕一郎、藤井 渉、真壁 秀文、藤井 博
    • Organizer
      第85回日本生化学会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-15
    • Related Report
      2012 Final Research Report
  • [Presentation] 大腸癌細胞におけるヒト核内受容体FXRの機能解析2012

    • Author(s)
      川村 悠気, 藤井 博
    • Organizer
      第85回日本生化学会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-15
    • Related Report
      2012 Final Research Report
  • [Presentation] Functional Analysis of the Farnesoid X receptor in Colorectal Cancer 大腸癌細胞における核内受容体 FXR の機能解析2012

    • Author(s)
      川村 悠気, 藤井 博
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-11
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト FABP5 遺伝子による癌の転移能獲得機構の 解 析 Functional Analysis of Acquisition Mechanism of Prostate Cancer Metastasis Mediated by Human FABP52012

    • Author(s)
      落合晋太郎, 川口 耕一郎、藤井 博
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-11
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト前立腺癌細胞における FABP5 遺伝子の発現制御機構の解析 Analysis of Regulatory Mechanisms of FABP5 Gene Expression in Human Prostate Cancer2012

    • Author(s)
      川口 耕一郎、木滑 歩、鈴木 俊介、藤井 博
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-11
    • Related Report
      2012 Final Research Report
  • [Presentation] 転移原因遺伝子FABP5 を標的とした抗腫瘍性分子の探索とその作用機構の解析 Identification and Characterization of a Novel Anti-tumorigenic Molecule Using the Metastasis-promoting Gene, FABP5, as a Molecular Target.2012

    • Author(s)
      戸田 一弥, 川口 耕一郎、藤井 渉、真壁 秀文、藤井 博
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-11
    • Related Report
      2012 Final Research Report
  • [Presentation] 青色光刺激を与えてキノコ菌糸の代謝経路を制御する2012

    • Author(s)
      小嶋 政信、藤井 博
    • Organizer
      第85回日本生化学会
    • Place of Presentation
      福岡
    • Related Report
      2012 Final Research Report
  • [Presentation] 青色光刺激を与えてキノコ菌糸の代謝経路を制御する2012

    • Author(s)
      小嶋 政信、藤井 博
    • Organizer
      第85回日本生化学会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] Characterization of metastasis-inducing gene, C-FABP/FABP5, overexpressed in human prostate cancer cells.2011

    • Author(s)
      川口 耕一郎, 藤井 博
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-14
    • Related Report
      2012 Final Research Report
  • [Presentation] 大腸癌細胞における核内受容体FXRの機能解析2011

    • Author(s)
      川村 悠気, 藤井 博
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-14
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト前立腺癌細胞におけるSIRT1の機能解析2011

    • Author(s)
      吉田 満, 藤井 博
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-14
    • Related Report
      2012 Final Research Report
  • [Presentation] Characterization of metastasis-inducing gene, C-FABP/FABP5, overexpressed in human prostate cancer cells2011

    • Author(s)
      川口耕一郎, 藤井博
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2011-12-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] 大腸癌細胞における核内受容体FXRの機能解析2011

    • Author(s)
      川村悠気, 藤井博
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2011-12-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒト前立腺癌細胞におけるSIRT1の機能解析2011

    • Author(s)
      吉田満, 藤井博
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2011-12-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Analysis of the mechanism of prostatic cancer metastasis mediated by human epidermal-type fatty acid-binding protein (C-FABP/FABP5).2011

    • Author(s)
      川口 耕一郎, 藤井 博
    • Organizer
      第84回日本生化学会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] Analysis of the mechanism of prostatic cancer metastasis mediated by human epidermal-type fatty scid-binding protein (C-FABP/FABP5)2011

    • Author(s)
      川口耕一郎, 藤井博
    • Organizer
      第84回日本生化学会
    • Place of Presentation
      京都国際会館(京都)(口頭)(ポスター)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Functional analysis of cancer promoting gene, human C-FABP/FABP5, in prostatecancer cells2010

    • Author(s)
      Koichiro Kawaguti, Hiroshi Fujii
    • Organizer
      第83回日本生化学会・第33回日本分子生物学会合同年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2012 Final Research Report
  • [Presentation] Functional analysis of cancer promoting gene, human C-FABP/FABP5, in prostate cancer cells2010

    • Author(s)
      Koichiro Kawaguti, Hiroshi Fujii
    • Organizer
      第83回日本生化学会・第33回日本分子生物学会合同年会
    • Place of Presentation
      神戸ポートアイランド(神戸)
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Functional Analysis of the Farnesoid X receptor in Colorectal Cancer 大腸癌細胞における核内受容体FXRの機能解析

    • Author(s)
      川村 悠気、藤井 博
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒトFABP5遺伝子による癌の転移能獲得機構の解析 Functional Analysis of Acquisition Mechanism of Prostate Cancer Metastasis Mediated by Human FABP5

    • Author(s)
      落合晋太郎、 川口 耕一郎、 藤井 博
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒト前立腺癌細胞におけるFABP5遺伝子の発現制御機構の解析 Analysis of Regulatory Mechanisms of FABP5 Gene Expression in Human Prostate Cancer

    • Author(s)
      川口 耕一郎、木滑 歩、鈴木 俊介、藤井 博
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] 転移原因遺伝子FABP5を標的とした抗腫瘍性分子の探索とその作用機構の解析Identification and Characterization of a Novel Anti-tumorigenic Molecule Using the Metastasis-promoting Gene, FABP5, as a Molecular Target.

    • Author(s)
      戸田 一弥、 川口 耕一郎、 藤井 渉、真壁 秀文、藤井 博
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒト前立腺癌細胞における転移原因遺伝子FABP5の発現制御機構の解析

    • Author(s)
      川口 耕一郎、木滑 歩、鈴木 俊介、藤井 博
    • Organizer
      第85回日本生化学会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] 転移原因遺伝子FABP5を分子標的とした抗腫瘍性分子の同定と作用機構の解析

    • Author(s)
      戸田 一弥、 川口 耕一郎、 藤井 渉、真壁 秀文、藤井 博
    • Organizer
      第85回日本生化学会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] 大腸癌細胞におけるヒト核内受容体FXRの機能解析

    • Author(s)
      川村 悠気、藤井 博
    • Organizer
      第85回日本生化学会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Annual Research Report

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Published: 2010-11-30   Modified: 2019-07-29  

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