Research Project
Grant-in-Aid for Scientific Research (C)
Co-crystallization of membrane proteins with antibody fragments may emerge as a general tool to facilitate crystal growth and improve crystal quality. The bound antibody fragment enlarges the hydrophilic part of the mostly hydrophobic membrane protein, thereby increasing the interaction area for possible protein-protein contacts in the crystal. To serve as a ‘crystallizing ligand’, the antibody fragment has to recognize an epitope that is only present in the native conformation (and not in the denatured state) of the membrane protein, bind with high affinity, and form stable and rigid complexes. In this project, we developed a fast and reliable method for generating crystallizing ligands against various mammalian G-protein coupled receptors (GPCRs) and transporters in a phage display format. Using this method, the crystal structure of a mammalian facilitative glucose transporter complexed with a Fv fragment has been determined at 3.5Åresolution.
All 2012 2011 2010
All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (3 results) Patent(Industrial Property Rights) (3 results) (of which Overseas: 1 results)
Nature
Volume: 482 Issue: 7384 Pages: 237-40
10.1038/nature10750
Micorb, Cell Fact.
Volume: 11 Issue: 1 Pages: 78-78
10.1186/1475-2859-11-78
Protein. Expr. Purif.
Volume: 79(1) Issue: 1 Pages: 81-7
10.1016/j.pep.2011.04.006
120003255719
Microbial Cell Factories
Volume: 10 Issue: 1 Pages: 24-24
10.1186/1475-2859-10-24
