Structure analysis of Nectin and Nectin like protein family
Project/Area Number |
22570115
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Structural biochemistry
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Research Institution | Osaka University |
Principal Investigator |
SUZUKI Mamoru 大阪大学, たんぱく質研究所, 准教授 (40280507)
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Project Period (FY) |
2010 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | 構造生物 / 細胞接着分子 / 構造生物学 / 細胞接着 / ネクチン様分子 / ネクチン / X線結晶学 |
Research Abstract |
We determined the crystal structures of the entire extracellular region of nectin-1,2,3. In the crystal, nectin formed a V-shaped homophilic dimer through the first Ig-like domain. Structure-based site-directed mutagenesis experimental results indicate that, in contrast with the previous notion, our structure represents a cis-dimer. Thus, our findings clearly reveal the structural basis for the cis-dimerization of nectins through the first Ig-like domains.
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] Crystal structure of a Bombyx mori Sigma-1 class glutathione transferase exhibiting prostaglandin E synthase activity2013
Author(s)
Yamamoto, K., Higashiura, A., Suzuki,M., Aritake, K.Urade, Y., Udome, N. Nakagawa, A
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Journal Title
Biochim Biophys Acta
Volume: 1830
Pages: 3711-3718
Related Report
Peer Reviewed
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[Journal Article] Structural basis for catalytic activity of a silkworm Delta-class glutathione transferase2012
Author(s)
Yamamoto, K., Usuda, K., Kakuta, Y., Makoto Kimura, M., Higashiura, A., Nakagawa, A., Suzuki, M
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Journal Title
Biochim Biophys Acta.
Volume: 1820
Pages: 1469-1474
Related Report
Peer Reviewed
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[Journal Article] Crystal structure of the cis-dimer of nectin-1 : Implications for the architecture of cell-cell junctions2011
Author(s)
Narita, H., Yamamoto, Y., Suzuki.M., Miyazaki, N., Yoshida, A., Kawai, K., Iwasaki, K., Nakagawa, A., Takai, Y., Sakisaka, T
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Journal Title
The Journal of Biological Chemistry
Volume: 286
Pages: 12659-12669
NAID
Related Report
Peer Reviewed
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[Journal Article] Refolding, crystallization and preliminary X-ray crystallographic study of the whole extracellular regions of nectins2011
Author(s)
Narita, H., Nakagawa, A., Yamamoto, Y., Sakisaka, T., Takai, Y., Suzuki.M.
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Journal Title
Acta Crystallographica Section F
Volume: 67
Pages: 344-348
Related Report
Peer Reviewed
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[Journal Article] Crystal structure of the cis-dimer of nectin-1 : Implications for the architecture of cell-cell junctions2011
Author(s)
Narita, H., Yamamoto, Y., Suzuki.M., Miyazaki, N., Yoshida, A., Kawai, K., Iwasaki, K., Nakagawa, A., Takai, Y., Sakisaka, T.
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Journal Title
The Journal of Biological Chemistry
Volume: 41(online)
NAID
Related Report
Peer Reviewed
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[Presentation] 細胞接着装置の制御に関わるLNX1PDZ2の構造・機能解析2012
Author(s)
中倉 由香子, 秋吉 由佳里, 合田 名都子, 成田 宏隆, 鈴木 守, 天野 剛志, 浜田大三, 藤原 芳江, 中川 敦史, 古瀬 幹夫, 廣明 秀一
Organizer
第35回日本分子生物学会年会
Place of Presentation
福岡
Related Report
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