Structural and functional analysis of GDE family proteins

• Project/Area Number: 22570117
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Osaka University
• Principal Investigator: AOYAMA Hiroshi 大阪大学, 大学院・薬学研究科, 准教授 (60291910)
• Co-Investigator(Renkei-kenkyūsha): OHSHIMA Noriyasu 群馬大学, 大学院・医学研究科, 助教 (30360514)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 膜タンパク質 / X 線結晶構造解析 / 構造生物学 / 脂質代謝 / X線結晶構造解析 / リン脂質

Research Abstract

For elucidation of the reaction mechanism of glecerophoshodiesterphosphodiesterase family protein (GDE1～7), studies on the structures and functions of GDE were carried out. Crystals of mouse membrane protein GDE1 were obtained in several new crystallization conditions including magnesium ion. These crystals diffracted up to 3.0Å resolution, and we are analyzing these data to determine the structure.

Research Products

• [Journal Article] 哺乳動物の GDE 群とマウス GDE5 の非酵素的機構による骨格筋分化制御 (2011)
  • Author(s): 大嶋紀安、岡崎優利、吉澤郁美、亀井康富、Stefania Mariggio、岡本佳子、立井一明、岸本幸治、小川佳宏、加藤範久、Daniela Corda、矢中規之、和泉孝志
  • Journal Title: 脂質生化学研究 Volume: 53 Pages: 208-209
  • NAID: 10028179005
• [Journal Article] A novel glycerophosophodiester phosphodiesterase GDE5 controls skeletal muscle development via a non-enzymatic mechanism (2010)
  • Author(s): Okazaki Yuri, Ohshima Noriyasu, Yoshizawa Ikumi, Kamei Yasutomi, Masiggio Stefania, Okamoto Keiko, Maeda Masahiro, Nogusa Yoshihito, Fujioka Yuichiro, Izumi Takashi, Ogawa Yoshihiro, Shiro Yoshitsugu, Wada Masanobu, Kato Norihisa, Corda
  • Journal Title: J. Biol. Chem Volume: 285 Pages: 27652-27663
  • Peer Reviewed
• [Presentation] マウス由来グリセロホスホジエステルホスホジエステラーゼ(GDE1) の基質特異性と組織分布の解析 (2010)
  • Author(s): 大嶋紀安、立井一明、金子真弓、城宜嗣、和泉孝志
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸
• [Remarks]
  • URL: http://www.phs.osaka-u.ac.jp/homepage/b006/
• [Remarks]
  • URL: http://www.phs.osaka-u.ac.jp/homepage/b006/