| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Research Abstract |
A combination of circular permutation and stopped-flow fluorescence analysis was utilized to probe the relationship between the molecular mechanism of GroEL-facilitated protein folding and the unique three-domain subunit structure of the GroEL protein. Through analysis of multiple circularly permuted GroEL variants, we succeeded in correlating a specific, domain-dependent conformational change to an important functional aspect of the chaperonin mechanism. Additionally, our results prompt a reevaluation of the conventional mechanism of GroEL-facilitated folding.
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