Structural analysis of supra-biomolecules by using ion mobility mass spectrometry
| Project/Area Number |
22570120
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Yokohama City University |
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Principal Investigator |
AKASHI Satoko 横浜市立大学, 大学院・生命ナノシステム科学研究科, 准教授 (10280728)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 質量分析 / タンパク質 / 生体分子 / 分析科学 / 蛋白質 |
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| Research Abstract |
Structural study of supra-biomoleculeswere executed by using ion mobility mass spectrometry (IM-MS), which provides not only of masses but also of collision cross-sections of the supra-biomolecules. Since IM-MS is applicable to theprotein complexes that are difficult to crystallize, it was possible to analyze theirstability and conformational alteration by IM-MS. In the present study, a calculation method of collision cross sectionsof dummy residue models for noncrystallizable supra-biomolecules was established.In addition, it was proved by IM-MS and molecular dynamics (MD) simulation that behaviors of the folded and unfolded regions in the gas phase were remarkably varied.
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Report
(4 results)
Research Products
(76 results)
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[Journal Article] Gas-phase structure of the histone multimers characterized by ion mobility mass spectrometry and molecular dynamics simulation2013
Author(s)
Saikusa, K., Fuchigami, S., Takahashi, K., Asano, Y., Nagadoi, A., Tachiwana, H., Kurumizaka, H., Ikeguchi, M., Nishimura, Y., *Akashi, S
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Journal Title
Anal. Chem
Volume: 85
Pages: 4165-4171
URL
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[Journal Article] Fission yeast Swi5-Sfr1 protein complex, an activator of Rad51 recombinase, forms an extremely elongated dogleg-shaped structure2011
Author(s)
Kokabu Y, Murayama Y, Kuwabara N, Oroguchi T, Hashimoto H, Tsutsui Y, Nozaki N, Akashi S, Unzai S, Shimizu T, Iwasaki H, Sato M, Ikeguchi M
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Journal Title
J Biol Chem
Volume: 286
Issue: 50
Pages: 43569-43576
DOI
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Peer Reviewed
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[Journal Article] Crystal structure of human REV7 in complex with a human REV3 fragment and structural implication of the interaction between DNA polymerase・and REV12010
Author(s)
Hara, K., *Hashimoto H., Murakumo, Y.,Kobayashi, S., Kogame, T., Unzai, S., Akashi, S., Takeda, S., Shimizu, T., Sato, M
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Journal Title
J. Biol. Chem
Volume: 285
Issue: 16
Pages: 12299-12307
DOI
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[Journal Article] Guidelines for reporting the use of capillary electrophoresis in proteomics2010
Author(s)
Domann PJ, Akashi S, Barbas C, Huang L, Lau W, Legido-Quigley C, McClean S, Neususs C, Perrett D, Quaglia M, Rapp E, Smallshaw L, Smith NW, Smyth WF, Taylor CF
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Journal Title
Nat Biotechnol
Volume: 28
Issue: 7
Pages: 654-655
DOI
Related Report
Peer Reviewed
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[Journal Article] Deimination stabilizes histone H2A/H2B dimers as revealed by electrospray ionization mass spectrometry2010
Author(s)
Shimoyama, S., Nagadoi, A., Tachiwana, H., Yamada, M., Sato, M., Kurumizaka, H., Nishimura, Y., Akashi, S.
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Journal Title
J.Mass Spectrom.
Volume: 45
Pages: 900-908
Related Report
Peer Reviewed
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[Journal Article] Guidelines for reporting the use of capillary electrophoresis in proteomics.2010
Author(s)
Domann, P.J., Akashi, S., Barbas, C., Huang, L., Lau, W, Legido-Quigley, C., McClean, S., Neususs, C., Perrett, D., Quaglia, M., Rapp, E., Smallshaw, L., Smith, N.W, Smyth, W.F., Taylor, C.F., Minimum Information About a Proteomics Experiment(MIAPE)
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Journal Title
Nat Biotechnol.
Volume: 28
Pages: 654-655
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[Presentation] What happens on the histone multimers in the gas phase?2012
Author(s)
Kazumi Saikusa, Sotaro Fuchigami, Kyohei Takahashi, Yuuki Asano, Aritaka Nagadoi, Hiroaki Tachiwana, Hitoshi Kurumizaka, Mitsunori Ikeguchi, Yoshifumi Nishimura, Satoko Akashi
Organizer
19th International Conference on Mass Spectrometry
Place of Presentation
Kyoto International Conference Center, Kyoto
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[Presentation] Characterization of histone multimers by ESI-MS2011
Author(s)
Shingo Shimoyama, Yuuki Asano, Kyohei Takahashi, Kazumi Saikusa, Aritaka Nagadoi, Hiroaki Tachiwana, Hitoshi Kurumizaka, Yoshifumi Nishimura, Satoko Akashi
Organizer
The 59th ASMS Conference on Mass Spectrometry and Allied Topics, poster
Place of Presentation
Denver
Year and Date
2011-06-02
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