Structural analysis of supra-biomolecules by using ion mobility mass spectrometry

• Project/Area Number: 22570120
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Yokohama City University
• Principal Investigator: AKASHI Satoko 横浜市立大学, 大学院・生命ナノシステム科学研究科, 准教授 (10280728)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 質量分析 / タンパク質 / 生体分子 / 分析科学 / 蛋白質

Research Abstract

Structural study of supra-biomoleculeswere executed by using ion mobility mass spectrometry (IM-MS), which provides not only of masses but also of collision cross-sections of the supra-biomolecules. Since IM-MS is applicable to theprotein complexes that are difficult to crystallize, it was possible to analyze theirstability and conformational alteration by IM-MS. In the present study, a calculation method of collision cross sectionsof dummy residue models for noncrystallizable supra-biomolecules was established.In addition, it was proved by IM-MS and molecular dynamics (MD) simulation that behaviors of the folded and unfolded regions in the gas phase were remarkably varied.

Research Products

• [Journal Article] Characterisation of an intrinsically disordered protein complex of Swi5-Sfr1 by ion mobility mass spectrometry and small-angle X-ray scattering (2013)
  • Author(s): Saikusa K, Kuwabara N, Kokabu Y, Inoue Y, Sato M, Iwasaki H, Shimizu T, Ikeguchi M, Akashi S
  • Journal Title: Analys Volume: 138 Issue: 5 Pages: 1441-14499
  • DOI: 10.1039/c2an35878f
  • NAID: 120006557136
  • Peer Reviewed
• [Journal Article] Gas-phase structure of the histone multimers characterized by ion mobility mass spectrometry and molecular dynamics simulation (2013)
  • Author(s): Saikusa, K., Fuchigami, S., Takahashi, K., Asano, Y., Nagadoi, A., Tachiwana, H., Kurumizaka, H., Ikeguchi, M., Nishimura, Y., *Akashi, S
  • Journal Title: Anal. Chem Volume: 85 Pages: 4165-4171
  • URL: http://dx.doi.org/10.1021/ac400395j
• [Journal Article] タンパク質複合体のイオンモビリティ質量分析 (2013)
  • Author(s): 明石知子、七種和美
  • Journal Title: エアロゾル研究 Volume: 28(2)(印刷中)
  • NAID: 130003367549
• [Journal Article] Gas-phase structure of the histone multimers characterized by ion mobility mass spectrometry and molecular dynamics simulation (2013)
  • Author(s): Saikusa K, Fuchigami S, Takahashi K, Asano Y, Nagadoi A, Tachiwana H, Kurumizaka H, Ikeguchi M, Nishimura Y, Akashi S
  • Journal Title: Anal Chem Volume: 85 Issue: 8 Pages: 4165-4171
  • DOI: 10.1021/ac400395j
  • Peer Reviewed
• [Journal Article] Impact of Limited Oxidation on Protein Ion Mobility and Structure of Importance to Footprinting by Radical Probe Mass Spectrometry (2012)
  • Author(s): Downard, K.M., Maleknia, S.D., Akashi, S.
  • Journal Title: Rapid Commun.Mass Spectrom. Volume: 26 Issue: 3 Pages: 226-230
  • DOI: 10.1002/rcm.5320
  • Peer Reviewed
• [Journal Article] Functions of homo- and hetero-oligomers of human Nucleoplasmin/Nucleophosmin family proteins NPM1, NPM2, and NPM3 during sperm chromatin remodeling (2012)
  • Author(s): Okuwaki M, Sumi A, Hisaoka M, Saotome-Nakamura A, Akashi S, Nishimura Y, Nagata K
  • Journal Title: Nucleic Acids Res Volume: 40 Issue: 11 Pages: 4861-4878
  • DOI: 10.1093/nar/gks162
  • Peer Reviewed
• [Journal Article] Crystal structures of penicillin-binding protein 3 (PBP3) from methicillin-resistant Staphylococcus aureus in the apo and cefotaxime-bound forms (2012)
  • Author(s): Yoshida H, Kawai F, Obayashi E, Akashi S, Roper DI, Tame JR, Park SY
  • Journal Title: J Mol Biol Volume: 423 Issue: 3 Pages: 351-364
  • DOI: 10.1016/j.jmb.2012.07.012
  • Peer Reviewed
• [Journal Article] Structural and biochemical analyses of the human PAD4 variant encoded by a functional haplotype gene (2011)
  • Author(s): Horikoshi N, Tachiwana H, Saito K, Osakabe A, Sato M, Yamada M, Akashi S, Nishimura Y, Kagawa W, Kurumizaka H
  • Journal Title: Acta Crystallogr D Biol Crystallog Volume: 67 Issue: 2 Pages: 112-118
  • DOI: 10.1107/s0907444910051711
  • Peer Reviewed
• [Journal Article] Homology Modelled Structure of the βB2B3-Crystallin Heterodimer Studied by Ion Mobility and Radical Probe Mass Spectrometry (2011)
  • Author(s): Downard, K.M., Kokabu, Y., Ikeguchi, M., Akashi, S.
  • Journal Title: FEBS J. Volume: 278 Issue: 21 Pages: 4044-4054
  • DOI: 10.1111/j.1742-4658.2011.08309.x
  • Peer Reviewed
• [Journal Article] Fission yeast Swi5-Sfr1 protein complex, an activator of Rad51 recombinase, forms an extremely elongated dogleg-shaped structure (2011)
  • Author(s): Kokabu Y, Murayama Y, Kuwabara N, Oroguchi T, Hashimoto H, Tsutsui Y, Nozaki N, Akashi S, Unzai S, Shimizu T, Iwasaki H, Sato M, Ikeguchi M
  • Journal Title: J Biol Chem Volume: 286 Issue: 50 Pages: 43569-43576
  • DOI: 10.1074/jbc.m111.303339
  • Peer Reviewed
• [Journal Article] 「質量分析によるタンパク質複合体の相互作用解析」生物工学 (2011)
  • Author(s): 明石知子
  • Journal Title: 日本生物工学会 Volume: 89(7) Pages: 384-387
• [Journal Article] Structural and biochemical analyses of the human PAD4 variant encoded by a functional haplotype gene (2011)
  • Author(s): Horikoshi N, Tachiwana H, Saito K, Osakabe A, Sato M, Yamado M, Akashi S, Nishimura Y, Kagawa W, Kurumizaka H.
  • Journal Title: Acta Cryst. Volume: D67 Pages: 112-118
  • Peer Reviewed
• [Journal Article] Crystal structure of human REV7 in complex with a human REV3 fragment and structural implication of the interaction between DNA polymerase・and REV1 (2010)
  • Author(s): Hara, K., *Hashimoto H., Murakumo, Y.,Kobayashi, S., Kogame, T., Unzai, S., Akashi, S., Takeda, S., Shimizu, T., Sato, M
  • Journal Title: J. Biol. Chem Volume: 285 Issue: 16 Pages: 12299-12307
  • DOI: 10.1074/jbc.m109.092403
• [Journal Article] Deimination stabilizes histone H2A/H2B dimers as revealed by electrospray ionization mass spectrometry (2010)
  • Author(s): Shimoyama S, Nagadoi A, Tachiwana H, Yamada M, Sato M, Kurumizaka H, Nishimura Y, Akashi S.
  • Journal Title: J Mass Spectrom Volume: 45 Issue: 8 Pages: 900-908
  • DOI: 10.1002/jms.1778
  • Peer Reviewed
• [Journal Article] Guidelines for reporting the use of capillary electrophoresis in proteomics (2010)
  • Author(s): Domann PJ, Akashi S, Barbas C, Huang L, Lau W, Legido-Quigley C, McClean S, Neususs C, Perrett D, Quaglia M, Rapp E, Smallshaw L, Smith NW, Smyth WF, Taylor CF
  • Journal Title: Nat Biotechnol Volume: 28 Issue: 7 Pages: 654-655
  • DOI: 10.1038/nbt0710-654b
  • Peer Reviewed
• [Journal Article] Deimination stabilizes histone H2A/H2B dimers as revealed by electrospray ionization mass spectrometry (2010)
  • Author(s): Shimoyama, S., Nagadoi, A., Tachiwana, H., Yamada, M., Sato, M., Kurumizaka, H., Nishimura, Y., Akashi, S.
  • Journal Title: J.Mass Spectrom. Volume: 45 Pages: 900-908
  • Peer Reviewed
• [Journal Article] Guidelines for reporting the use of capillary electrophoresis in proteomics. (2010)
  • Author(s): Domann, P.J., Akashi, S., Barbas, C., Huang, L., Lau, W, Legido-Quigley, C., McClean, S., Neususs, C., Perrett, D., Quaglia, M., Rapp, E., Smallshaw, L., Smith, N.W, Smyth, W.F., Taylor, C.F., Minimum Information About a Proteomics Experiment(MIAPE)
  • Journal Title: Nat Biotechnol. Volume: 28 Pages: 654-655
• [Journal Article] Crystal structure of human REV7 in complex with a human REV3 firagment and structural implication of the interaction between DNA polymerase ζ and REV1 (2010)
  • Author(s): Hera K, Hashimoto H, Murakumo Y, Kobayashi S, Kogame T, Unzai S, Akashi S, Takeda S, Shimizu T, Sato M.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 12299-12307
  • Peer Reviewed
• [Presentation] Characterization of IDPs by Mass Spectrometry and MD simulation (2013)
  • Author(s): Satoko Akashi
  • Organizer: 2nd International Symposium on Intrinsically Disordered Proteins
  • Place of Presentation: Riken Yokohama Institute Koryuto Hall (Yokohama)
• [Presentation] 質量分析による構造生物学 (2013)
  • Author(s): 明石知子
  • Organizer: 中性子連携研究会（第4 回中性子小角散乱解析法研究会）
  • Place of Presentation: 京都大学東京オフィス（東京）
  • Invited
• [Presentation] 高分子としてのタンパク質構造解析:様々の手法のシンクロナイゼーション質量分析の立場から (2012)
  • Author(s): 明石知子
  • Organizer: 第6回ソフトマター中性子散乱研究会
  • Place of Presentation: 東京
  • Year and Date: 2012-12-21
• [Presentation] 質量分析による構造生物学 (2012)
  • Author(s): 明石知子
  • Organizer: 中性子連携研究会(第4回中性子小角散乱解析法研究会)
  • Place of Presentation: 東京
  • Year and Date: 2012-03-14
• [Presentation] イオンモビリティ質量分析によるIDP の構造解析-Swi5-Sfr1 の気相中での構造- (2012)
  • Author(s): 七種和美、明石知子
  • Organizer: 新学術領域研究「天然変性タンパク質の分子認識機構と機能発現」第2回公開シンポジウム
  • Place of Presentation: 吹田
  • Year and Date: 2012-01-24
• [Presentation] Behavior of Intrinsically Disordered Regions within a Protein Complex of Swi5-Sfr1 Characterized by Ion Mobility Mass Spectrometry (2012)
  • Author(s): Kazumi Saikusa, Naoyuki Kuwabara, Yuichi Kokabu, Mamoru Sato, Hiroshi Iwasaki, Mitsunori Ikeguchi, Toshiyuki Shimizu, Satoko Akashi
  • Organizer: The 60th ASMS Conference on Mass Spectrometry and Allied Topics, poster
  • Place of Presentation: Vancouver
• [Presentation] Application of Homology Modelling, Ion Mobility and Protein Footprinting in Unison to Characterize Protein Bimolecular Complexes (2012)
  • Author(s): Yuichi Kokabu, Mitsunori Ikeguchi, Satoko Akashi, Kevin Downard
  • Organizer: The 60th ASMS Conference on Mass Spectrometry and Allied Topics, poster
  • Place of Presentation: Vancouver
• [Presentation] Kevin Downard, Impact of Protein Oxidation on their Ion Mobility for Protein Footprinting Applications (2012)
  • Author(s): Simin Maleknia, Satoko Akashi
  • Organizer: The 60th ASMS Conference on Mass Spectrometry and Allied Topics, poster
  • Place of Presentation: Vancouver
• [Presentation] What happens on the histone multimers in the gas phase? (2012)
  • Author(s): Kazumi Saikusa, Sotaro Fuchigami, Kyohei Takahashi, Yuuki Asano, Aritaka Nagadoi, Hiroaki Tachiwana, Hitoshi Kurumizaka, Mitsunori Ikeguchi, Yoshifumi Nishimura, Satoko Akashi
  • Organizer: 19th International Conference on Mass Spectrometry
  • Place of Presentation: Kyoto
• [Presentation] Behavior of intrinsically disordered regions within a protein complex of Swi5-Sfr1 characterized by IM-MS and SAXS (2012)
  • Author(s): Kazumi Saikusa, Naoyuki Kuwabara, Yuichi Kokabu, Mamoru Sato, Hiroshi Iwasaki, Mitsunori Ikeguchi, Toshiyuki Shimizu, Satoko Akashi
  • Organizer: Behavior of intrinsically disordered regions within a protein complex of Swi5-Sfr1 characterized by IM-MS and SAXS
  • Place of Presentation: Kyoto
• [Presentation] Structure of a Beta-Crystallin Heterodimer by Ion Mobility and Radical Probe Mass Spectrometry (2012)
  • Author(s): Kevin Downard, Yuichi Kokabu, Mitsunori Ikeguchi, Satoko Akashi
  • Organizer: 19th International Conference on Mass Spectrometry
  • Place of Presentation: Kyoto
• [Presentation] Characterization of histone multimers in the gas phase by ion mobility mass spectrometry and molecular dynamics simulation (2012)
  • Author(s): Kazumi Saikusa, Sotaro Fuchigami, Kyohei Takahashi, Yuuki Asano, Aritaka Nagadoi, Hiroaki Tachiwana, Hitoshi Kurumizaka, Mitsunori Ikeguchi, Yoshifumi Nishimura, Satoko Akashi
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋大学(愛知県)
• [Presentation] 質量分析を用いたヌクレオソームのアセチル化に伴う構造変化の解析 (2012)
  • Author(s): 畔上奈々子、七種和美、神蔵祐典、戸所泰人、立和名博昭、長土居有隆、胡桃坂仁志、西村善文、明石知子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] Characterization of IDPs by Mass Spectrometry and MD simulation (2012)
  • Author(s): Satoko Akashi
  • Organizer: 2nd International Symposium on Intrinsically Disordered Proteins
  • Place of Presentation: Yokohama
• [Presentation] Behavior of Intrinsically Disordered Regions within a Protein Complex of Swi5-Sfr1 Characterized by Ion Mobility Mass Spectrometry (2012)
  • Author(s): Kazumi Saikusa, Naoyuki Kuwabara, Yuichi Kokabu, Mamoru Sato, Hiroshi Iwasaki, Mitsunori Ikeguchi, Toshiyuki Shimizu, and Satoko Akashi
  • Organizer: The 60th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Vancouver Convention Center, Canada
• [Presentation] Application of Homology Modelling, Ion Mobility and Protein Footprinting in Unison to Characterize Protein Bimolecular Complexes (2012)
  • Author(s): Yuichi Kokabu, Mitsunori Ikeguchi, Satoko Akashi, Kevin Downard
  • Organizer: The 60th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Vancouver Convention Center, Canada
• [Presentation] Impact of Protein Oxidation on their Ion Mobility for Protein Footprinting Applications (2012)
  • Author(s): Simin Maleknia, Satoko Akashi, Kevin Downard
  • Organizer: The 60th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Vancouver Convention Center, Canada
• [Presentation] What happens on the histone multimers in the gas phase? (2012)
  • Author(s): Kazumi Saikusa, Sotaro Fuchigami, Kyohei Takahashi, Yuuki Asano, Aritaka Nagadoi, Hiroaki Tachiwana, Hitoshi Kurumizaka, Mitsunori Ikeguchi, Yoshifumi Nishimura, Satoko Akashi
  • Organizer: 19th International Conference on Mass Spectrometry
  • Place of Presentation: Kyoto International Conference Center, Kyoto
• [Presentation] Behavior of intrinsically disordered regions within a protein complex ofSwi5-Sfr1 characterized by IM-MS and SAXS (2012)
  • Author(s): Kazumi Saikusa, Naoyuki Kuwabara, Yuichi Kokabu, Mamoru Sato, Hiroshi Iwasaki, Mitsunori Ikeguchi, Toshiyuki Shimizu, Satoko Akashi
  • Organizer: 19th International Conference on Mass Spectrometry
  • Place of Presentation: Kyoto International Conference Center, Kyoto
• [Presentation] Structure of a Beta-Crystallin Heterodimer by Ion Mobility and Radical Probe Mass Spectrometry (2012)
  • Author(s): Kevin Downard, Yuichi Kokabu, Mitsunori Ikeguchi, Satoko Akashi
  • Organizer: 19th International Conference on Mass Spectrometry
  • Place of Presentation: Kyoto International Conference Center, Kyoto
• [Presentation] 質量分析を用いたヌクレオソームのアセチル化に伴う構造変化の解析 (2012)
  • Author(s): 畔上奈々子、七種和美、神蔵祐典、戸所泰人、立和名博昭、長土居有隆、胡桃坂仁志、西村善文、明石知子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場（福岡）
• [Presentation] 高分子としてのタンパク質構造解析：様々の手法のシンクロナイゼーション －質量分析の立場から－ (2012)
  • Author(s): 明石知子
  • Organizer: 第6回ソフトマター中性子散乱研究会
  • Place of Presentation: 研究社英語センタービル 大会議室（東京）
  • Invited
• [Presentation] イオンモビリティ質量分析によるIDPの構造解析-Swi5-Sfr1の気相中での構造- (2012)
  • Author(s): 七種和美、明石知子
  • Organizer: 新学術領域研究「天然変性タンパク質の分子認識機構と機能発現」第2回公開シンポジウム
  • Place of Presentation: 吹田・千里ライフサイエンスセンター
• [Presentation] 質量分析で観測されるヒストンテイルの挙動 (2012)
  • Author(s): 七種和美、明石知子, 他6名
  • Organizer: 新学術領域研究「天然変性タンパク質の分子認識機構と機能発現」第2回公開シンポジウム
  • Place of Presentation: 吹田・千里ライフサイエンスセンター
• [Presentation] 質量分析で観るタンパク質の姿 (2011)
  • Author(s): 明石知子
  • Organizer: 理研シンポジウム第12回分析・解析技術と化学の最先端(依頼講演)
  • Place of Presentation: 和光
  • Year and Date: 2011-12-12
• [Presentation] 質量分析で観るタンパク質の姿 (2011)
  • Author(s): 明石知子
  • Organizer: 理研シンポジウム第12回分析・解析技術と化学の最先端
  • Place of Presentation: 和光・理化学研究所(招待講演)
  • Year and Date: 2011-12-12
• [Presentation] Characterization of histone multimers by ESI-MS (2011)
  • Author(s): Shingo Shimoyama, Yuuki Asano, Kyohei Takahashi, Kazumi Saikusa, Aritaka Nagadoi, Hiroaki Tachiwana, Hitoshi Kurumizaka, Yoshifumi Nishimura, Satoko Akashi
  • Organizer: The 59th ASMS Conference on Mass Spectrometry and Allied Topics, poster
  • Place of Presentation: Denver
  • Year and Date: 2011-06-02
• [Presentation] 質量分析によるタンパク質複合体の相互作用解析 (2011)
  • Author(s): 明石知子
  • Organizer: 第1回生体分子相互作用解析フォーラム(依頼講演)
  • Place of Presentation: 東京
  • Year and Date: 2011-03-10
• [Presentation] 質量分析によるタンパク質複合体の相互作用解析 (2011)
  • Author(s): 明石知子
  • Organizer: 第1回生体分子相互作用解析フォーラム
  • Place of Presentation: 東京・ベックマンコールター本社
  • Year and Date: 2011-03-10
• [Presentation] Mass Spectrometry for Characterization of IDPs (2011)
  • Author(s): Satoko Akashi
  • Organizer: The 1st International Symposium on Intrinsically Disordered Proteins(依頼講演)
  • Place of Presentation: 横浜・はまぎんホールヴィアマーレ
  • Year and Date: 2011-01-27
• [Presentation] Mass Spectrometry for Characterisation of IDPs (2011)
  • Author(s): Satoko Akashi
  • Organizer: The 1^<st> International Symposium on Intrinsically Disordercd Proteins
  • Place of Presentation: 横浜・はまぎんホールヴィアマーレ
  • Year and Date: 2011-01-27
• [Presentation] Mass Spectrometry of Histone Multimers (2011)
  • Author(s): Satoko Akashi
  • Organizer: The 2nd Asian Oceanic Mass Spectrometry Conference (2nd AOMSC) (invited)
  • Place of Presentation: Busan
• [Presentation] イオンモビリティ質量分析とX線小角散乱による基本転写因子TFIIEの構造解析 (2011)
  • Author(s): 七種和美、小田隆、奥田昌彦、池口満徳、佐藤衛、西村善文、明石知子
  • Organizer: 第59回質量分析総合討論会
  • Place of Presentation: 吹田
• [Presentation] 機能するタンパク質の構造変化を質量分析で探る (2011)
  • Author(s): 明石知子
  • Organizer: 公開シンポジウム「蛋白質の機能を解き明かす多彩なアプローチ」(依頼講演)
  • Place of Presentation: 横浜
• [Presentation] 機能するタンパク質の構造変化を質量分析で探る (2011)
  • Author(s): 明石知子
  • Organizer: 公開シンポジウム「蛋白質の機能を解き明かす多彩なアプローチ」
  • Place of Presentation: 横浜・横浜市立大学鶴見キャンパス(招待講演)
• [Presentation] イオンモビリティ質量分析とX線小角散乱による基本転写因子TFIIEの構造解析 (2011)
  • Author(s): 七種和美、小田隆、奥田昌彦、池口満徳、佐藤衛、西村善文、明石知子
  • Organizer: 第59回質量分析総合討論会(2011)
  • Place of Presentation: 吹田・阪急エキスポパークホテル
• [Presentation] Mass Spectrometry of Histone Multimers (2011)
  • Author(s): Satoko Akashi
  • Organizer: The 2nd Asian Oceanic Mass Spectrometry Conference (2nd AOMSC)
  • Place of Presentation: Busan・Busan Exhibition & Convention Center (BEXCO)(招待講演)
• [Presentation] Characterization of histone multimers by ESI-MS (2011)
  • Author(s): Shingo Shimoyama, Satoko Akashi, 他7名
  • Organizer: The 59th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Denver, Colorado Convention Center
• [Presentation] タンパク質複合体を観る-イオンモビリティMSの実際- (2010)
  • Author(s): 明石知子
  • Organizer: BMSシンポジウム「機能するタンパク質の構造を探る」
  • Place of Presentation: 東京
  • Year and Date: 2010-12-11
• [Presentation] タンパク質複合体を観る-イオンモビリティMSの実際- (2010)
  • Author(s): 明石知子
  • Organizer: BMSシンポジウム「機能するタンパク質の構造を探る」
  • Place of Presentation: 東京・(財)微生物化学研究所
  • Year and Date: 2010-12-11
• [Presentation] 質量分析を用いたピストンH2A-H2Bのシトルリン化の解析 (2010)
  • Author(s): 浅野裕輝、下山真吾、長土居有隆、立和名博昭、山田道之、佐藤衛、胡桃坂仁志、西村善文、明石知子
  • Organizer: 日本分子生物学会年会・日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] 転写制御に関わる天然変性タンパク質の質量分析による構造機能解析 (2010)
  • Author(s): 明石知子
  • Organizer: 酵素工学研究会第64回講演会(依頼講演)
  • Place of Presentation: 東京
  • Year and Date: 2010-11-19
• [Presentation] 転写制御に関わる天然変性タンパク質の質量分析による構造機能解析 (2010)
  • Author(s): 明石知子
  • Organizer: 酵素工学研究会第64回講演会
  • Place of Presentation: 東京・東京大学山上会館
  • Year and Date: 2010-11-19
• [Presentation] 翻訳後修飾に伴う蛋白質の構造変化-H2A/H2B のシトルリン化- (2010)
  • Author(s): 下山真吾、長土居有隆、立和名博昭、浅野裕輝、山田道之、佐藤衛、胡桃坂仁志、西村善文、明石知子
  • Organizer: 第58回質量分析総合討論会
  • Place of Presentation: つくば
  • Year and Date: 2010-06-16
• [Presentation] 翻訳後修飾に伴う蛋白質の構造変化-H2A/H2Bのシトルリン化- (2010)
  • Author(s): 下山真吾、長土居有隆、立和名博昭、浅野裕輝、山田道之、佐藤衛、胡桃坂仁志、西村善文、明石知子
  • Organizer: 第58回質量分析総合討論会(2010)&1^<st> Asian and Oceanic Mass Spectrometry Conference
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2010-06-16
• [Presentation] Analysis ofTRAP-antiTRAP interactions by sedimentation velocity analytical ultracentrifugation (2010)
  • Author(s): Satoru Unzai, Masahiro Watanabe, Satoko Akashi, Jonathan Heddle, Sam-Yong Park, Jeremy R.H. Tame
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台・東北大学川内キャンパス
• [Presentation] Mass spectrometry ofDNA G-quadruplex bound to a human telomeric protein, hTRF2 (2010)
  • Author(s): Naohiro Kodama, Yuuka Hirao, Noriyuki Iwasaki, Yoshiyuki Itoh, Yoshifumi Nishimura, Satoko Akashi
  • Organizer: 第37回国際核酸化学シンポジウム(ISNAC 2010)
  • Place of Presentation: 横浜・はまぎんホールヴィアマーレ
• [Presentation] 質量分析を用いた生体超分子の構造機能研究-構造生物学から見たCEへの期待- (2010)
  • Author(s): 明石知子
  • Organizer: 第30回キャピラリー泳動シンポジウム(依頼講演)
  • Place of Presentation: 岐阜・長良川国際会議場
• [Presentation] Analysis of TRAP-antiTRAP interactions by sedimentation velocity analytical ultracentrifugation (2010)
  • Author(s): Satoru Unzai, Masahiro Watanabe, Satoko Akashi, Jonathan Heddle, Sam-Yong Park, Jeremy R.H.Tame
  • Organizer: 第48回日本生物物理学会年会(2010)
  • Place of Presentation: 仙台市・東北大学川内キャンパス
• [Presentation] Mass spectrometry of DNA G-quadruplex bound to a human telomeric protein, hTRF2 (2010)
  • Author(s): Naohiro Kodama, Yuuka Hirao, Nonyuki Iwasaki, Yoshiyuki Itoh, Yoshifumi Nishimwa, Satoko Akastu
  • Organizer: 第37回国際核酸化学シンポジウム(ISNAC 2010)
  • Place of Presentation: 横浜・はまぎんホールヴィアマーレ
• [Presentation] 質量分析を用いた生体超分子の構造機能研究-構造生物学から見たCEへの期待- (2010)
  • Author(s): 明石知子
  • Organizer: 第30回キャピラリー泳動シンポジウム
  • Place of Presentation: 岐阜・長良川国際会議場
• [Book] 現代質量分析学、高山光男、早川滋雄、瀧浪欣彦、和田芳直編第26章生体超分子 (2013)
  • Author(s): 明石知子
  • Publisher: 化学同人
• [Book] “現代質量分析学”、第26章 生体超分子 (2013)
  • Author(s): 明石知子
  • Publisher: 化学同人
• [Book] 試料分析講座タンパク質分析、日本分析化学会編5.3タンパク質複合体のネイティブ質量分析 (2012)
  • Author(s): 明石知子
  • Publisher: 丸善出版
• [Book] “試料分析講座 タンパク質分析”、日本分析化学会編 5.3 タンパク質複合体のネイティブ質量分析 (2012)
  • Author(s): 明石知子
  • Publisher: 丸善出版
• [Book] 翻訳後修飾のプロテオミクス-質量分析装置を中心とした分析法の原理-、平野久、大野茂男編4章4.13.2 質量分析法による脱イミノ化の検出 (2011)
  • Author(s): 明石知子
  • Publisher: 講談社
• [Book] 翻訳後修飾のプロテオミクス-質量分析装置を中心とした分析法の原理-、4章4.13.2質量分析法による脱イミノ化の検出(平野久、大野茂男編) (2011)
  • Author(s): 明石知子
  • Publisher: 講談社
• [Remarks] ホームページ
  • URL: http://www-mls.tsurumi.yokohama-cu.ac.jp/stbiol/