| Project/Area Number |
22570121
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
|
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| Research Institution | Kyoto Prefectural University |
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Principal Investigator |
ODA Masayuki 京都府立大学, 大学院・生命環境科学研究科, 准教授 (20318231)
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| Co-Investigator(Renkei-kenkyūsha) |
UCHIYAMA Susumu 大阪大学, 大学院・工学研究科, 准教授 (90335381)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 分子認識 / 生体分子 / タンパク質 / 分析化学 / 免疫学 / 蛋白質 |
|---|
| Research Abstract |
Antibodies recognize various types of antigens, different shapes and sizes, and are good targets for the analysis to elucidate the structural dynamics of proteins. In this study, I changed the structures of protein antigens by chemical modification and disulfide-bonddeletion, and analyzed how the antibodies could recognize the slight and dynamic differences in antigens using the methods of binding kinetics and thermodynamics. As a result, I could detect the plural states of protein antigens and antibodies in solution, and quantitatively determine the effects of protein fluctuations.
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