| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
For elucidation of the mechanisms of huge Complex I, locates at the entrance of the mitochondrial respiratory chains, determination of the atomic structure of the whole Complex I is essential. We have established the method for purification of intact Complex I from bovine heart and surveyed the 2-D and 3-D crystallization conditions to provide the crystals of the enzyme complex. The resonance Raman spectra of FMN in purified enzyme were determined, suggest the existence of a mechanism for prevention of spontaneous production of reactive oxygen species.
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