Elucidation of the reaction mechanisms for mitochondrial respiratory complex I
| Project/Area Number |
22570122
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | University of Hyogo |
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Principal Investigator |
ITOH Kyoko (SHINZAWA Kyoko) 兵庫県立大学, 大学院・生命理学研究科, 助教 (70206316)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | X 線結晶解析 / 膜タンパク質 / ミトコンドリア呼吸鎖 / ２次元結晶 / ラマン分光法 / フラビン / 鉄硫黄クラスター / X線結晶構造 / 2次元結晶 |
|---|
| Research Abstract |
For elucidation of the mechanisms of huge Complex I, locates at the entrance of the mitochondrial respiratory chains, determination of the atomic structure of the whole Complex I is essential. We have established the method for purification of intact Complex I from bovine heart and surveyed the 2-D and 3-D crystallization conditions to provide the crystals of the enzyme complex. The resonance Raman spectra of FMN in purified enzyme were determined, suggest the existence of a mechanism for prevention of spontaneous production of reactive oxygen species.
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Report
(4 results)
Research Products
(79 results)
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[Presentation] cbb3 がたチトクロム酸化酵素の発現条件と精製2011
Author(s)
Yuriko Ando,Hideo Shimada,Kyoko Shinzawa-Itoh,Robert Gennis,Shinya Yoshikawa,Kazumasa Muramoto
Organizer
日本生物物理学会
Place of Presentation
兵庫県立大学・姫路書写キャンパス(兵庫県)
Year and Date
2011-09-17
Related Report
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[Presentation] 界面活性剤によるウシ心筋チトクロム酸化酵素の結晶化条件の検討2011
Author(s)
Miki Hatanaka,Tomonori Misaki,Takashi Sugimura,Kyoko Shinzawa-Itoh, Kazumasa Muramoto,Eiki Yamashita,Tomitake Tukihara,Shinya Yoshikawa
Organizer
日本生物物理学会
Place of Presentation
兵庫県立大学・姫路書写キャンパス(兵庫県)
Year and Date
2011-09-16
Related Report
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[Presentation] ウシ心筋 NADH-ユビキノン酸化還元酵素の完全性が二次元結晶の質に及ぼす影響2010
Author(s)
Satoru Shimada, Masahide Hikita,Kyoko Shinzawa-Itoh, Christoph Gerle,Kazutoshi Tani, Yoshinori Fujiyoshi, Atuo Miyazawa, Shinya Yoshikawa
Organizer
日本生物物理学会
Place of Presentation
東北大学・川内北キャンパス(宮城県)
Year and Date
2010-09-22
Related Report
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[Presentation] 界面活性剤がウシ心筋複合体Iの2次元結晶へ及ぼす影響2010
Author(s)
Satoko Amano, Satoru Shimada, Masahide Hikita, Kyoko Shinzawa-Itoh, Christoph Gerle, Yoshinori Fujiyoshi, Atuo Miyazawa, Shinya Yoshikawa
Organizer
日本生物物理学会
Place of Presentation
東北大学・川内北キャンパス(宮城県)
Year and Date
2010-09-22
Related Report
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