Interaction between Innate Immune Receptor CD14 and Ligand LPS on Membranes

• Project/Area Number: 22570127
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Suntory Foundation for Life Sciences
• Principal Investigator: 三浦 薫 (野村 薫 / 三浦 薫(野村薫)) 公益財団法人サントリー生命科学財団, 生物有機科学研究所, 研究員 (90353515)
• Co-Investigator(Renkei-kenkyūsha): SHIMAMOTO Keiko 公益財団法人サントリー生命科学財団, 生物有機科学研究所, 主幹研究員 (70235638) ; KUSUMOTO Shoichi 公益財団法人サントリー生命科学財団, 生物有機科学研究所, 所長 (30028253)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: GPIアンカー / 固体NMR法 / 脂質二重膜 / 自然免疫 / 酵母培養系 / GPIアンカー型蛋白質 / 固体ＮＭＲ

Research Abstract

To complete the methodology, we changed the procedure into using a model membrane. We succeeded in obtaining high resolution solid-state NMR spectra of membrane samples in which pseudo GPI-anchored proteins were inserted

Research Products

• [Journal Article] "Hydration and Temperature Dependence of 13C and 1H NMR Spectra of the DMPC Phospholipid Membrane and Complete Resonance Assignment of Its Crystalline State." (2011)
  • Author(s): K. Nomura, M. Lintuluoto, and K. Morigaki
  • Journal Title: J. Phys.Chem Volume: vol. 115 Pages: 14991-15001
  • Peer Reviewed
• [Journal Article] "Lipopolysaccharide induces raft domain expansion in membrane composed of a phospholipid-cholesterol-sphingomyelin ternary system." (2011)
  • Author(s): K. Nomura, M. Maeda, K. Sugase and S.Kusumoto
  • Journal Title: Innate Immun Volume: vol. 17 Pages: 256-268
  • Peer Reviewed
• [Journal Article] "Fluorinated cholesterol retains domain-forming activity in sphingomyelin bilayers." (2011)
  • Author(s): N. Matsumori, H. Okazaki, K. Nomura and M. Murata
  • Journal Title: Chem. Phys. Lipids Volume: vol. 164 Pages: 401-408
  • Peer Reviewed
• [Journal Article] Hydration and Temperature Dependence of ^<13>C and ^1H NMR Spectra of the DMPC Phospholipid Membrane and Complete Resonance Assignment of Its Crystalline State (2011)
  • Author(s): Nomura K., Lintuluoto M., Morigaki K.
  • Journal Title: J.Phys.Chem.B. Volume: Vol.115 Issue: 50 Pages: 14991-15001
  • DOI: 10.1021/jp208958a
  • Peer Reviewed
• [Journal Article] Lipopolysaccharide induces raft domain expansion in membrane composed of a phospholipid-cholesterol-sphingomyelin ternary system (2011)
  • Author(s): Nomura K., Maeda M., Sugase K., Kusumoto S.
  • Journal Title: Innate Immun. Volume: Vol.17 Issue: 3 Pages: 256-268
  • DOI: 10.1177/1753425910365944
  • Peer Reviewed
• [Journal Article] Fluorinated cholesterol retains domain-forming activity in sphingomyel in bilayers (2011)
  • Author(s): Matsumori N., Okazaki H., Nomura K., Murata M.
  • Journal Title: Chem.Phys.Lipids. Volume: Vol.164 Issue: 5 Pages: 401-408
  • DOI: 10.1016/j.chemphyslip.2011.05.007
  • Peer Reviewed
• [Presentation] "Hydration and Temperature Dependence of 13C and 1H NMR Spectra of the DMPC Phospholipid Membrane and Complete Resonance Assignment of Its Crystalline State." (2011)
  • Author(s): K. Nomura
  • Organizer: International ERATO Symposium on Lipid Structures in and around Proteins (招待講演)
  • Place of Presentation: ホテル阪急エキスポパーク、吹田市
• [Presentation] "Hydration and Temperature Dependence of 13C and 1H NMR Spectra of the DMPC Phospholipid Membrane and Complete Resonance Assignment of Its Crystalline State." (2011)
  • Author(s): K. Nomura
  • Organizer: The International Symposium on Nuclear Magnetic Resonance 2011(招待講演)
  • Place of Presentation: 大桟橋ホール、横浜市
• [Presentation] Hydration and Temperature Dependence of ^<13>C and ^1H NMR Spectra of the DMPC Phospholipid Membrane and Complete Resonance Assignment of Its Crystalline State (2011)
  • Author(s): Nomura K.
  • Organizer: International ERATO Symposium on Lipid Structures in and around Proteins
  • Place of Presentation: ホテル阪急エキスポパーク(吹田市)(招待講演)
• [Presentation] Hydration and Temperature Dependence of ^<13>C and ^1H NMR Spectra of the DMPC Phospholipid Membrane and Complete Resonance Assignment of Its Crystalline State (2011)
  • Author(s): Nomura K.
  • Organizer: The International Symposium on Nuclear Magnetic Resonance 2011
  • Place of Presentation: 大桟橋ホール(横浜市)
• [Presentation] "自然免疫の鍵物質リポ多糖と脂質膜の相互作用解析 (2010)
  • Author(s): 野村 薫
  • Organizer: "固体NMR材料フォーラム「―固体NMR研究の最前線―」(招待講演)
  • Place of Presentation: 神戸製鉄所、神戸市
• [Presentation] "Lipopolysaccharide induces raft domain expansion in a phospholipid-cholesterol-sphingomyelin ternary system." (2010)
  • Author(s): K. Nomura
  • Organizer: system."SLB (Society of Leukocyte Biology) / IEIIS (International Endotoxin and Innate Immunity Society) 2010 Annual meeting
  • Place of Presentation: Vancouver、 Canada
• [Presentation] "Lipopolysaccharide induces raft domain expansion in a phospholipid-cholesterol-sphingomyelin ternary system." (2010)
  • Author(s): K. Nomura
  • Organizer: PACIFICHEM 2010 Advances in Solid-State NMR of Biomlogical Molecules.(招待講演)
  • Place of Presentation: Honolulu, USA
• [Presentation] 自然免疫の鍵物質リポ多糖と脂質膜の相互作用解析 (2010)
  • Author(s): 野村薫
  • Organizer: 固体NMR材料フォーラム
  • Place of Presentation: 神戸市(神戸製鉄所)
• [Presentation] Lipopolysaccharide induces raft domain expansion in a phospholipid-cholesterol-sphingomyelin ternary system. (2010)
  • Author(s): Kaoru Nomura, et al.
  • Organizer: SLB(Society of Leukocyte Biology)/IEIIS(International Endotoxin and Innate Immunity Society)2010 Annual meeting
  • Place of Presentation: Vancouver Canada
• [Presentation] Lipopolysaccharide induces raft domain expansion in a phospholipid-cholesterol-sphingomyelin ternary system. (2010)
  • Author(s): Kaoru Nomura
  • Organizer: PACIFICHEM 2010 Advances in Solid-State NMR of Biomlogical Molecules.
  • Place of Presentation: Honolulu, USA.
• [Book] "Lipopolysaccharide Induces Raft Domain Expansion in a Cholesterol-ContainingMembrane"in "Advances in Biological Solid-State NMR: Proteins and Membrane-Active Peptides"
  • Author(s): K. Nomura and S. Kusumoto
  • Publisher: RSC Publishing,
• [Remarks]
  • URL: http://www.sunbor.or.jp
• [Remarks]
  • URL: http://www.sunbor.or.jp
• [Remarks]
  • URL: http://www.sunbor.or.jp