Role of long-chain polyunsaturated fatty acids in the membrane transport in bacteria
| Project/Area Number |
22570130
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
OKUYAMA Hidetoshi 北海道大学, 大学院・地球環境科学研究院, 准教授 (90125295)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 生体膜 / 長鎖多価不飽和脂肪酸 / EPA / 膜透過性 / TolC-AcrAB / 異物排出系 / 抗酸化 / チオエステラーゼ / pfa遺伝子 / 多価不飽和脂肪酸合成酵素 / 膜遮蔽効果 / 酸化ストレス / TolC-AcrABタンパク質 / 抗生物質 / DHA / 細胞膜 / 膜排出タンパク質 |
|---|
| Research Abstract |
To investigate the function of eicosapentaenoic acid (EPA) in the bacterial cell membrane, Escherichia coli lacking either tolC, acrA, or acrB gene was transformed with the EPA biosynthesis pfa genes. The susceptibility of these recombinants possessing EPA against antibiotics was compared with that of their reference cells without EPA. Recombinants with EPA were more resistant to antibiotics than their references without EPA. Electrophoresis of the hydrophobic protein fraction of the recombinants indicated that more AcrA protein was accumulated in the cells with EPA than in those without EPA, suggesting that EPA might be involved in the exclusion of antibiotics by the TolC-AcrAB system in the bacterial cell membranes.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Shewanella oshoroensis sp. nov., a mesophilic eicosapentaenoic acid and hentriacontanonaene-producing bacterium. Res2012
Author(s)
Sugihara S, Bin Haji Mohd Taha AI, Motoigi T, Ueno, A, Shimizu, T, Kaneko, T, Watanabe, K, Yumoto, I, and O k uy a ma , H
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Journal Title
J. Microbiol
Volume: 7
Issue: 2
Pages: 131-138
DOI
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Peer Reviewed
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[Book] Lipids in Cold-adapted Microorganisms.2013
Author(s)
(Bin Haji Mohd Taha, AI), Ahmed, R. Z., Motoigi, T, Watanabe, K, Kurosawa, N, Okuyama, H
Total Pages
26
Publisher
Caister Academic Press
Related Report
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[Book] Innovations in Biotechnology2012
Author(s)
(Bin Haji Mohd Taha, AI), Okuyama, H, Ohwada, T, Yumoto, I, and Orikasa, Y
Total Pages
25
Publisher
Exogenous Catalase Gene Expression as a Tool for Enhancing Metabolic Activity and Production of Biomaterials in Host Microorganisms
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