Relationship between inhibition of ID1 expression and induction of irreversible suppression of cell proliferation in HepG2 hepatoma cells treated with HGF
| Project/Area Number |
22570137
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
TANAKA Toshiaki 東京工業大学, 大学院・生命理工学研究科, 助教 (40263446)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Shuji 東京大学, 教養学部, 特任准教授 (90447318)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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|---|
| Keywords | ホルモンと生理活性物質 / 肝細胞増殖因子 / 癌 / ID1 / 不可逆的増殖停止 / Skp2 / Myc / 遺伝子発現 / エピジェネティクス / シグナル伝達 / 除放剤 / 転写制御 / プロモーターアッセイ / HepG2 |
|---|
| Research Abstract |
HGF treatment of HepG2 hepatoma cells decreases expression of an oncogene ID1. This study showed that the decrease in ID1 is regulated through downregulation of Skp2, a component of E3 ubiquitin ligase, in the presence of HGF. However, Skp2 was involved in the regulation of ID1 expression by regulating transcription activity of Myc independently of ubiquitination. This study also showed that G9a histone methyltransferase, which expression may be regulated through Skp2/Myc/ID1, is involved in the alteration of H3K9me3 localization in cell nuclei induced by HGF, leading to the irreversible suppression of cancer cell proliferation by HGF.
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Report
(4 results)
Research Products
(63 results)
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[Journal Article] Critical role of farnesoid X receptor (FXR) for hepatocellular carcinoma cell Proliferation.2012
Author(s)
T.Fujino, A.Takeuchi, A.Maruko-Ohtake, Y.Ohtake, J.Satoh, T.Kobayashi, T.Tanaka, H.Ito, R.Sakamaki, R.Kashimura, K.Ando, T.Nishimaki-Mogami, Y.Ohkubo, N.Kitamura, R.Sato, K.Kikugawa, and M.Hayakawa.
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Journal Title
Journal of Biochemistry
Volume: 152
Pages: 577-586
Related Report
Peer Reviewed
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[Journal Article] Critical role of farnesoid X receptor (FXR) for hepatocellular carcinoma cell Proliferation.2012
Author(s)
T.Fujino, A.Takeuchi, A.Maruko-Ohtake, Y.Ohtake, J.Satoh,T.Kobayashi, T.Tanaka, H.Ito, R.Sakamaki, R.Kashimura, K. Ando, T.Nishimaki-Mogami, Y.Ohkubo, R.Sato, K.Kikugawa, M.Hayakawa.
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Journal Title
Journal of Biochemistry
Volume: 152
Issue: 6
Pages: 577-586
DOI
Related Report
Peer Reviewed
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[Presentation] What we can do with over 300 billion bases of RNA-seq data: The case of Xenopus laevis genome project2013
Author(s)
T.Kwon, S.Takahashi, T.Tanaka, H.Noguchi, A.Toyoda, A.Fujiyama, Y.Suzuki, N.Ueno, M.Taira, J.B.Wallingford, E.M. Marcotte.
Organizer
International Symposium on Genome Science
Place of Presentation
The University of Tokyo
Year and Date
2013-01-09
Related Report
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[Presentation] What we can do with over 300 billion bases of RNA-seq data: The case of Xenopus laevis genome project.2013
Author(s)
T.Kwon, S.Takahashi, T.Tanaka, H.Noguchi, A.Toyoda, A.Fujiyama, Y.Suzuki, N.Ueno, M.Taira, J.B.Wallingford, E.M.Marcotte.
Organizer
International Symposium on Genome Science
Place of Presentation
The University of Tokyo (Tokyo)
Related Report
Invited
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[Presentation] Analysis of the Xenopus laevis J-strain genome by BAC end sequencing, FISH, and RNA-sequence2012
Author(s)
S. Takahashi, A. Toyoda, Y. Kuroki, Y. Uno, Y. Izutsu, A. Suzuki, T. Michiue, H. Ogino, H. Ochi, T. Tanaka, A. Fukui, Y. Ito, N. Ueno, M. Asashima, Y. Matsuda, M. Taira, A. Fujiyama
Organizer
The14th International Xenopus Conference
Place of Presentation
Giens Peninsula,France
Year and Date
2012-09-10
Related Report
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[Presentation] Massively parallel regulation of head and non-head genes by Otx2, Lim1 and Gsc underlies the evolution of the head organizer in the chordate2012
Author(s)
Y.Yasuoka, Y.Suzuki, S.Takahashi, N.Sudou, Y.Haramoto, Y.Tandou, K.Kubokawa, K.W. Cho, M.Asashima, S.Sugano, M.Taira
Organizer
第45回日本発生生物学会大会
Place of Presentation
神戸国際会議場(兵庫県)
Related Report
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[Presentation] Analysis of the Xenopus laevis J-strain genome by BAC end sequencing, FISH, and RNA-sequence.2012
Author(s)
S.Takahashi, A.Toyoda, Y.Kuroki, Y.Uno, Y Izutsu, A.Suzuki, T.Michiue, H.Ogino, H Ochi, T.Tanaka, A.Fukui, Y.Ito, N.Ueno, M.Asashima, Y.Matsuda, M.Taira, A.Fujiyama
Organizer
The 14th International Xenopus Conference
Place of Presentation
Giens Peninsula, France
Related Report
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[Presentation] Massively parallel regulation of head and non-head genes by Otx2, Lim1 and Gsc underlies the evolution of the head organizer in the chordate.2012
Author(s)
Y.Yasuoka, Y.Suzuki, S.Takahashi, N.Sudou, Y.Haramoto, Y.Tandou, K.Kubokawa, K.W. Cho, M.Asashima, S.Sugano, M.Taira
Organizer
第45回日本発生生物学会大会
Place of Presentation
神戸国際会議場（兵庫県）
Related Report
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