Studies of action mechanisms of radical enzyme systems for providing new paradigms of enzyme researches
| Project/Area Number |
22570143
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Okayama University |
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Principal Investigator |
TORAYA Tetsuo 岡山大学, 大学院・自然科学研究科, 特命教授 (70026318)
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| Co-Investigator(Kenkyū-buntansha) |
TOBIMATSU Takamasa 岡山大学, 大学院・自然科学研究科, 准教授 (30188768)
MORI Koichi 岡山大学, 大学院・自然科学研究科, 助教 (50379715)
SERA Takashi 京都大学, 大学院・工学研究科, 准教授 (10362443)
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| Co-Investigator(Renkei-kenkyūsha) |
YOSHIZAWA Kazunari 九州大学, 先導物質化学研究所, 教授 (30273486)
SHIBATA Naoki 兵庫県立大学, 大学院・生命理学研究科, 准教授 (30295753)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ラジカル酵素 / B_12補酵素 / S-アデノシルメチオニン / 酵素反応機構 / 再活性化因子 / 活性化蛋白質 / 立体構造 / システム酵素学 / B12補酵素 / 酵素 / B_<12>補酵素 / 酵素の触媒機構 / X線結晶解析 / 理論計算 / 酵素反応 |
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| Research Abstract |
Where should enzyme sciences be directed? I believe that
(1) microbe-inspired system enzymology and
(2)理論化学との連携による酵素学の非経験的学問化、が今後の酵素研究に重要なパラダイムであると考えて、ラジカル酵素システムを研究対象として、これらの方向への展開を目指した。
In this research project, our investigations of radical enzymes and their (re)activating proteins using the methodologies of biochemistry, genetic engineering, biophysics, X-ray crystallography, and theoretical chemistry opened a new horizon on enzyme sciences.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] Expression, Crystallization and Preliminary X-ray Crystallographic Study of Ethanolamine Ammonia-lyase from Escherichia coli2010
Author(s)
N. Shibata, H. Tamagaki, S. Ohtsuki, N. Hieda, K. Akita, H. Komori, Y. Shomura, S. Terawaki, T. Toraya, N. Yasuoka and Y. Higuchi
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Journal Title
Acta Crystallogr.
Volume: F66
Issue: 6
Pages: 709-711
DOI
Related Report
Peer Reviewed
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[Journal Article] Crystal Structures of Ethanolamine Ammonia-lyase complexed with Coenzyme B_12 Analogs and Substrates2010
Author(s)
N. Shibata, H. Tamagaki, N. Hieda, K. Akita, H. Komori, Y. Shomura, S. Terawaki, K. Mori, N. Yasuoka, Y. Higuchi, and T. Toraya
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Journal Title
J. Biol. Chem
Volume: 285 (34)
Issue: 34
Pages: 26484-26493
DOI
Related Report
Peer Reviewed
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