Coordination mechanism of multiple motor proteins : a DNA-protein hybrid nanosystem study

• Project/Area Number: 22570156
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: The University of Tokyo
• Principal Investigator: TADAKUMA Hisashi 東京大学, 大学院・新領域創成科学研究科, 助教 (10339707)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 1分子計測・操作 / ナノマシン / 蛋白質 / 核酸 / 生物物理 / 1分子計測(SMD) / モーター蛋白質 / ナノシステム

Research Abstract

The motor protein kinesin drive vesicle transport in the cell using energy derived from ATP. However, how multiple kinesin are coordinated each other remains elusive. Here we created a hybrid nanomachine(DNA-Tile-kinesin) using DNA-Tile as the skeletal structure and kinesin as the functional module. Single molecule imaging of DNA-Tile-kinesin hybrid allowed us to evaluate the effects of the number of molecules involved in the transport and nanometer changes in the distance between multiple kinesins on motility. Our results show that number of kinesin participating in the transport affect on run length but not on velocity. These results indicate the power of our approach toward the understanding of coordination mechanism of motor proteins.

Research Products

• [Journal Article] Single molecule imaging of the trans-translation entry process via anchoring of the tagged ribosome (2011)
  • Author(s): Zhou ZP, Shimizu Y, Tadakuma H*, Taguchi H, Ito K, Ueda T
  • Journal Title: Journal of Biochemistry Volume: 149(5) Pages: 609-618
  • NAID: 10030585460
  • Peer Reviewed
• [Journal Article] Real time monitoring of endogenous cytoplasmic mRNA using linear antisense 2'-O-methyl RNA probes in living cells. (2011)
  • Author(s): Okabe K, Harada Y, Zhang J, Tadakuma H, Tani T, Funatsu T
  • Journal Title: Nucleic Acids Research Volume: 39(4)
  • Peer Reviewed
• [Journal Article] Single molecule imaging of the trans-translation entry process via anchoring of the tagged ribosome (2011)
  • Author(s): Zhou ZP, Shimizu Y, Tadakuma H, Taguchi H, Ito K, Ueda T
  • Journal Title: Journal of Biochemistry Volume: (印刷中,印刷中 掲載確定)
  • NAID: 10030585460
  • Peer Reviewed
• [Journal Article] Real time monitoring of endogenous cytoplasmic mRNA using linear antisense 2^'-O-methyl RNA probes in living cells. (2011)
  • Author(s): Okabe K, Harada Y, Zhang J, Tadakuma H, Tani T, Funatsu T.
  • Journal Title: Nucleic Acids Research Volume: 39(4)
  • Peer Reviewed
• [Presentation] 設計して調整する分子アクチュエータ:DNA-蛋白質ハイブリッド・ナノマシンの構築 (2012)
  • Author(s): 多田隈尚史
  • Organizer: 日本生化学会
  • Place of Presentation: 国立京都国際会館(招待講演)
  • Year and Date: 2012-09-21
• [Presentation] Constructing DNA-kinesin hybrid-nanomachine using the DNA-tile scaffold (2012)
  • Author(s): Miyazono Yuya, Endo Masayuki, Ueda Takuya, Sugiyama Hiroshi, Harada
  • Organizer: 日本生物物理学会
  • Place of Presentation: 兵庫県立大学・姫路書写キャンパス(兵庫県)
  • Year and Date: 2012-09-16
• [Presentation] 設計して調整する分子アクチュエータ: DNA-蛋白質ハイブリッド・ナノマシンの構築 (2011)
  • Author(s): 多田隈尚史
  • Organizer: 日本生化学会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-09-21
• [Presentation] Constructing DNA-kinesin hybrid-nanomachine using the DNA-tile scaffold (2011)
  • Author(s): Miyazono Yuya, Endo Masayuki, Ueda Takuya, Sugiyama Hiroshi, Harada Yoshie, Tadakuma Hisashi
  • Organizer: 日本生物物理学会
  • Place of Presentation: 兵庫県立大学・姫路書写キャンパス(兵庫県)
  • Year and Date: 2011-09-16
• [Presentation] Single molecule imaging of the trans-translation entry process via anchoring of the tagged ribosome (2010)
  • Author(s): Zhou ZP, Yao C, Shimizu Y, Tadakuma H, Taguchi H, Ito K, Ueda T
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会合同大会)
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-08
• [Presentation] Single molecule imaging of the trans-translation entry process via anchoring of the tagged ribosome (2010)
  • Author(s): Zhou ZP, Yao C, Shimizu Y, Tadakuma H, Taguchi H, Ito K, Ueda T
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-08
• [Presentation] Strain through the neck linker ensures processive runs : a DNA-kinesin hybrid nanomachine study (2010)
  • Author(s): Miyazono Y, Hayashi M, Karagiannis P, Harada Y, Tadakuma H
  • Organizer: 日本生物物理学会
  • Place of Presentation: 東北大学川内キャンパス(宮城県)
  • Year and Date: 2010-09-21
• [Presentation] 転写・翻訳系がカップルしたin vitro無細胞翻訳系再構成の試み構成の試み (2010)
  • Author(s): Tanaka K, Matsui J, Koyama H, Tamaru D, Tadakuma H, Shimizu Y, Ueda T
  • Organizer: 日本生物物理学会
  • Place of Presentation: 東北大学川内キャンパス(宮城県)
  • Year and Date: 2010-09-21
• [Presentation] 転写・翻訳系がカップルしたin vitro無細胞翻訳系再構成の試み (2010)
  • Author(s): Tanaka K, Matsui J, Koyama H, Tamaru D, Tadakuma H, Shimizu Y, Ueda T
  • Organizer: 日本生物物理学会
  • Place of Presentation: 東北大学川内キャンパス(宮城県)
  • Year and Date: 2010-09-21
• [Presentation] 1分子イメージングによるトランスートランスレーションにおけるSmpBのC末端の機能解析 (2010)
  • Author(s): Zhou ZP, Suto Y, Shimizu Y, Tadakuma H, Taguchi H, Ito K, Ueda T
  • Organizer: 日本生物物理学会
  • Place of Presentation: 東北大学川内キャンパス(宮城県)
  • Year and Date: 2010-09-20
• [Presentation] Functional analysis of the C-terminus region of SmpB in trans-translation by single-molecule imaging (2010)
  • Author(s): Zhou ZP, Shimizu Y, Tadakuma H, Ito K, Taguchi H, Ueda T
  • Organizer: 日本RNA学会
  • Place of Presentation: 一橋記念講堂(東京都)
  • Year and Date: 2010-07-28
• [Presentation] Functional analysis of the C-terminus region of SmpB in trans-translation by single-molecule imaging (2010)
  • Author(s): Zhou ZP, Shimizu Y, Tadakuma H, Ito K, Taguchi H, Ueda T
  • Organizer: 日本RNA学界
  • Place of Presentation: 一橋記念講堂(東京都)
  • Year and Date: 2010-07-28