| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
We analyzed the structure and function of minor-type dyneins whose axonemal contents are lower than other major-type dyneins. Here we found that one of three minor dyneins more strongly bind to microtubules than most of Chlamydomonas dyneins in the absence of ATP. In addition, using cryoelectron tomography and subtomogram averaging, we found that each minor dynein locates to the ciliary proximal region in place of a specific major dynein. Furthermore, we found that a novel gigantic dynein, which has been the last dynein heavy chain remained to be identified in Chlamydomonas, localizes to the proximal region of one of nine doublet microtubules. This is the first report that all the gene products of dynein heavy chain have been identified in the singleorganism.
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