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Organelle imaging of signal transduction towards necrotic cell death

Research Project

Project/Area Number 22570158
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

OHTA Yoshihiro  東京農工大学, 大学院工学研究院, 准教授 (10223843)

Co-Investigator(Renkei-kenkyūsha) UMEDA Norihiro  東京農工大学, 大学院工学研究院, 教授 (60111803)
Project Period (FY) 2010-04-01 – 2013-03-31
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsバイオイメージング / オルガネラ / ミトコンドリア / 蛍光イメージング / ネクローシス / シクロフィリンD / 呼吸 / 活性酵素種 / エネルギー代謝 / 酸化ストレス / 膜透過性遷移
Research Abstract

In order to investigate the mechanism of intracellular signal transduction towards necrosis, we measured the effects of cyclophilin D on the energy metabolism of mitochondria and the changes of mitochondria observed upon induction of necrosis. The changes were detected by imaging single mitochondria with optical microscopy. As a result, we succeeded in 1) detection of the effects of cyclophilin D on the pyruvate metabolism in cells, 2) detection of long-lasting mitochondrial permeability transition in cells, and 3) simultaneous imaging of mitochondrial permeability transition and mitochondrial swelling. These results provide important information on the mechanism of necrosis as programmed cell death.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (28 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (9 results) (of which Peer Reviewed: 7 results) Presentation (17 results) (of which Invited: 2 results) Remarks (2 results)

  • [Journal Article] Detection of swelling of single isolated mitochondrion with optical microscopy2014

    • Author(s)
      Morikawa D, Kanematsu K., Shibata T., Haseda K., Umeda N., and Ohta Y
    • Journal Title

      Biomedical Optics Express

      Volume: 5 Pages: 848-857

    • Related Report
      2012 Final Research Report
  • [Journal Article] pH measurement using dual wavelength fluorescent ratio by two-photon excitation for mitochondrial activity2012

    • Author(s)
      Y. Kanazashi, Y. Li, T. Onojima, K. Iwami, Y. Ohta, N. Umeda
    • Journal Title

      Jpn. J. Appl. Phys

      Volume: 51 Issue: 11R Pages: 117011-117015

    • DOI

      10.1143/jjap.51.117001

    • NAID

      40019496267

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] ミトコンドリアの内部密度の評価法の開発とその応用2012

    • Author(s)
      長谷田圭亮、兼松啓太、太田善浩
    • Journal Title

      バイオイメージング

      Volume: 21 Pages: 15-21

    • NAID

      10031161857

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Observation of Mitochondrial Activity Based on Temporal and Spatial pH Variation Measured by Near-Field Fluorescent Ratiometry2011

    • Author(s)
      Yongbo Li, Ryosuke Shinohara, Kentaro Iwami, Yoshihiro Ohta a n d Norihiro Umeda
    • Journal Title

      Science China Physics, Mechanics and Astronomy

      Volume: 54 Issue: 12 Pages: 2225-2229

    • DOI

      10.1007/s11433-011-4533-4

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Two-photon fluorescence near-field pH measurement for mitochondria activity2011

    • Author(s)
      Kanazashi Y., Li Y., Onojima T., Iwami K., Ohta Y., and Umeda N
    • Journal Title

      Proc. SPIE

      Volume: 8105 Pages: 81050E-81050E

    • DOI

      10.1117/12.893359

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dual Wavelength Fluorescent Ratiometric pH Measurement by Scanning Near-Field Optical Microscopy2011

    • Author(s)
      Li Y., Shinohara R., Iwami K., Ohta Y. and Umeda N
    • Journal Title

      Proceedings of SPIE

      Volume: 7544 Pages: 7544191-6

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Observation of Mitochondrial Activity based on Time and Spatial pH Variation Measured by Near-Field Fluorescent Ratiometry2011

    • Author(s)
      Li Y., Shinohara R., Iwami K., Ohta Y., Umeda N.
    • Journal Title

      Science China, Physics, Mechanics and Astronomy

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Experimental Protocols for Function Analysis of Isolated Presynaptic Terminals2010

    • Author(s)
      Tezaki M. and Ohta Y
    • Journal Title

      Clinical Nuroscience

      Volume: 28 Pages: 889-891

    • Related Report
      2012 Final Research Report
  • [Journal Article] 酸化ストレスによる細胞内ミトコンドリアの内膜透過性遷移の観察2010

    • Author(s)
      松野本宜寛、石暁磊、岩崎あすか、太田善浩
    • Journal Title

      バイオイメージング

      Volume: 18 Pages: 278-283

    • NAID

      10027006695

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] ミトコンドリアの一時的脱分極の細胞内における誘導と観察2013

    • Author(s)
      海内 寛嗣
    • Organizer
      第 2 回生物物理関東支部研究会
    • Place of Presentation
      東京農工大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] Cyclophilin D の PPIase 活性によるミトコンドリアの質と量の変化2013

    • Author(s)
      小林 明日香
    • Organizer
      第 2 回生物物理関東支部研究会
    • Place of Presentation
      東京農工大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 細胞分裂時のミトコンドリア膜電位のモニタリング2013

    • Author(s)
      蛭崎 琴絵
    • Organizer
      第 2 回生物物理関東支部研究会
    • Place of Presentation
      東京農工大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] The imaging analysis of mitochondria with retardation mediated differential interference microscope (RM-DIC)2012

    • Author(s)
      Haseda K., Kanematsu K., and Ohta Y
    • Organizer
      日本生物物理学会第50回年会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2012-09-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Effects of the crowding of mitochondria on their ATP production2012

    • Author(s)
      Yoshimatsu D., and Ohta Y
    • Organizer
      日本生物物理学会第50回年会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2012-09-24
    • Related Report
      2012 Final Research Report
  • [Presentation] シナプス小胞のH+勾配形成におけるミトコンドリアの役割2012

    • Author(s)
      太田善浩
    • Organizer
      日本薬学会第132会年会 シンポジウム「ミトコンドリアへの薬物の作用と創薬について考える」
    • Place of Presentation
      北海道大学(札幌)
    • Year and Date
      2012-03-29
    • Related Report
      2012 Final Research Report
    • Invited
  • [Presentation] 位相変調型微分干渉顕微鏡を用いたミトコンドリアのイメージング解析2012

    • Author(s)
      Keisuke Haseda
    • Organizer
      日本生物物理学会第50回年回
    • Place of Presentation
      名古屋大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] ミトコンドリアの密集がATP合成に及ぼす影響2012

    • Author(s)
      Daiki Yoshimatsu
    • Organizer
      日本生物物理学会第50回年回
    • Place of Presentation
      名古屋大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] ミトコンドリアの一時的脱分極は活性酸素発生の抑制機構として働く2011

    • Author(s)
      太田善浩、小野島匠
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2011-09-22
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] Effects of cyclophilin D on energy metabolism of cells2011

    • Author(s)
      Osaki, H., Masuguchi, T., Nakazato, H., Matsunomoto, Y., Fujita, C., and Ohta, Y
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学
    • Year and Date
      2011-09-17
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] Novel technique for study on the mechanism of mitochondrial volume maintenance2011

    • Author(s)
      Kanematsu K., Kaji, T., and Ohta, Y
    • Organizer
      第49回日本生物物理学会年会
    • Place of Presentation
      兵庫県立大学
    • Year and Date
      2011-09-16
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] Mitochondria reduce the production of ROS by their transient depolarizations2011

    • Author(s)
      Ohta Y
    • Organizer
      The 8th Conference of Asian Society for Mitochondrial Research and Medicine
    • Place of Presentation
      鹿児島市民文化ホール
    • Year and Date
      2011-09-04
    • Related Report
      2012 Final Research Report
    • Invited
  • [Presentation] Mitochondria reduce the production of ROS by their transient depoalrizations2011

    • Author(s)
      Ohta, Y, Onojima, T.
    • Organizer
      5th SFRE-Asia, 8th ASMRM, 11th J-mit
    • Place of Presentation
      鹿児島市民文化ホール(招待講演)
    • Year and Date
      2011-09-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] An attempt to measure pH around mitochondria by using fluorescence microscopy with near field optical probe2010

    • Author(s)
      Haseda K., Li Y., Shinohara R., Kanezashi Y., Onojima T., Iwami K., Umeda N. and Ohta Y
    • Organizer
      第48回日本生物物理学会年会
    • Place of Presentation
      東北大学
    • Year and Date
      2010-09-20
    • Related Report
      2012 Final Research Report
  • [Presentation] An attempt to measure pH around mitochondria by using fluorescence microscopy with near field optical probe2010

    • Author(s)
      Haseda K., Li Y., Shinohara R., Kanezashi Y., Onojima T., Iwami K., Umeda N., Ohta Y.
    • Organizer
      第48回日本生物物理学会年会
    • Place of Presentation
      東北大学, 仙台市
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] Wavelength Fluorescent Ratiometric pH Measurement by Scanning Near-Field Optical Microscopy2010

    • Author(s)
      Li Y, Shinohara R., Iwami K., Ohta Y. and Umeda, N. Dual
    • Organizer
      6th International Symposium on Precision Engineering Measurements and Instrumentation (ISPEMI 2010)
    • Place of Presentation
      Hangzhou, China
    • Year and Date
      2010-08-10
    • Related Report
      2012 Final Research Report
  • [Presentation] Dual Wavelength Fluorescent Ratiometric pH Measurement by Scanning Near-Field Optical Microscopy2010

    • Author(s)
      Li Y, Shinohara R., Iwami K., Ohta Y., Umeda、N.
    • Organizer
      6th International Symposium on Precision Engineering Measurements and Instrumentation (ISPEMI 2010)
    • Place of Presentation
      Hangzhou, China
    • Year and Date
      2010-08-10
    • Related Report
      2010 Annual Research Report
  • [Remarks] 太田研究室

    • URL

      http://www.tuat.ac.jp/~ohta/

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.tuat.ac.jp/~ohta/

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2016-11-25  

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