Organelle imaging of signal transduction towards necrotic cell death

• Project/Area Number: 22570158
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: OHTA Yoshihiro 東京農工大学, 大学院工学研究院, 准教授 (10223843)
• Co-Investigator(Renkei-kenkyūsha): UMEDA Norihiro 東京農工大学, 大学院工学研究院, 教授 (60111803)
• Project Period (FY): 2010-04-01 – 2013-03-31
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: バイオイメージング / オルガネラ / ミトコンドリア / 蛍光イメージング / ネクローシス / シクロフィリンD / 呼吸 / 活性酵素種 / エネルギー代謝 / 酸化ストレス / 膜透過性遷移

Research Abstract

In order to investigate the mechanism of intracellular signal transduction towards necrosis, we measured the effects of cyclophilin D on the energy metabolism of mitochondria and the changes of mitochondria observed upon induction of necrosis. The changes were detected by imaging single mitochondria with optical microscopy. As a result, we succeeded in 1) detection of the effects of cyclophilin D on the pyruvate metabolism in cells, 2) detection of long-lasting mitochondrial permeability transition in cells, and 3) simultaneous imaging of mitochondrial permeability transition and mitochondrial swelling. These results provide important information on the mechanism of necrosis as programmed cell death.

Research Products

• [Journal Article] Detection of swelling of single isolated mitochondrion with optical microscopy (2014)
  • Author(s): Morikawa D, Kanematsu K., Shibata T., Haseda K., Umeda N., and Ohta Y
  • Journal Title: Biomedical Optics Express Volume: 5 Pages: 848-857
• [Journal Article] pH measurement using dual wavelength fluorescent ratio by two-photon excitation for mitochondrial activity (2012)
  • Author(s): Y. Kanazashi, Y. Li, T. Onojima, K. Iwami, Y. Ohta, N. Umeda
  • Journal Title: Jpn. J. Appl. Phys Volume: 51 Issue: 11R Pages: 117011-117015
  • DOI: 10.1143/jjap.51.117001
  • NAID: 40019496267
  • Peer Reviewed
• [Journal Article] ミトコンドリアの内部密度の評価法の開発とその応用 (2012)
  • Author(s): 長谷田圭亮、兼松啓太、太田善浩
  • Journal Title: バイオイメージング Volume: 21 Pages: 15-21
  • NAID: 10031161857
  • Peer Reviewed
• [Journal Article] Observation of Mitochondrial Activity Based on Temporal and Spatial pH Variation Measured by Near-Field Fluorescent Ratiometry (2011)
  • Author(s): Yongbo Li, Ryosuke Shinohara, Kentaro Iwami, Yoshihiro Ohta a n d Norihiro Umeda
  • Journal Title: Science China Physics, Mechanics and Astronomy Volume: 54 Issue: 12 Pages: 2225-2229
  • DOI: 10.1007/s11433-011-4533-4
  • Peer Reviewed
• [Journal Article] Two-photon fluorescence near-field pH measurement for mitochondria activity (2011)
  • Author(s): Kanazashi Y., Li Y., Onojima T., Iwami K., Ohta Y., and Umeda N
  • Journal Title: Proc. SPIE Volume: 8105 Pages: 81050E-81050E
  • DOI: 10.1117/12.893359
  • Peer Reviewed
• [Journal Article] Dual Wavelength Fluorescent Ratiometric pH Measurement by Scanning Near-Field Optical Microscopy (2011)
  • Author(s): Li Y., Shinohara R., Iwami K., Ohta Y. and Umeda N
  • Journal Title: Proceedings of SPIE Volume: 7544 Pages: 7544191-6
  • Peer Reviewed
• [Journal Article] Observation of Mitochondrial Activity based on Time and Spatial pH Variation Measured by Near-Field Fluorescent Ratiometry (2011)
  • Author(s): Li Y., Shinohara R., Iwami K., Ohta Y., Umeda N.
  • Journal Title: Science China, Physics, Mechanics and Astronomy Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Experimental Protocols for Function Analysis of Isolated Presynaptic Terminals (2010)
  • Author(s): Tezaki M. and Ohta Y
  • Journal Title: Clinical Nuroscience Volume: 28 Pages: 889-891
• [Journal Article] 酸化ストレスによる細胞内ミトコンドリアの内膜透過性遷移の観察 (2010)
  • Author(s): 松野本宜寛、石暁磊、岩崎あすか、太田善浩
  • Journal Title: バイオイメージング Volume: 18 Pages: 278-283
  • NAID: 10027006695
  • Peer Reviewed
• [Presentation] ミトコンドリアの一時的脱分極の細胞内における誘導と観察 (2013)
  • Author(s): 海内 寛嗣
  • Organizer: 第 2 回生物物理関東支部研究会
  • Place of Presentation: 東京農工大学
• [Presentation] Cyclophilin D の PPIase 活性によるミトコンドリアの質と量の変化 (2013)
  • Author(s): 小林 明日香
  • Organizer: 第 2 回生物物理関東支部研究会
  • Place of Presentation: 東京農工大学
• [Presentation] 細胞分裂時のミトコンドリア膜電位のモニタリング (2013)
  • Author(s): 蛭崎 琴絵
  • Organizer: 第 2 回生物物理関東支部研究会
  • Place of Presentation: 東京農工大学
• [Presentation] The imaging analysis of mitochondria with retardation mediated differential interference microscope (RM-DIC) (2012)
  • Author(s): Haseda K., Kanematsu K., and Ohta Y
  • Organizer: 日本生物物理学会第50回年会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2012-09-24
• [Presentation] Effects of the crowding of mitochondria on their ATP production (2012)
  • Author(s): Yoshimatsu D., and Ohta Y
  • Organizer: 日本生物物理学会第50回年会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2012-09-24
• [Presentation] シナプス小胞のH+勾配形成におけるミトコンドリアの役割 (2012)
  • Author(s): 太田善浩
  • Organizer: 日本薬学会第132会年会 シンポジウム「ミトコンドリアへの薬物の作用と創薬について考える」
  • Place of Presentation: 北海道大学(札幌)
  • Year and Date: 2012-03-29
  • Invited
• [Presentation] 位相変調型微分干渉顕微鏡を用いたミトコンドリアのイメージング解析 (2012)
  • Author(s): Keisuke Haseda
  • Organizer: 日本生物物理学会第50回年回
  • Place of Presentation: 名古屋大学
• [Presentation] ミトコンドリアの密集がATP合成に及ぼす影響 (2012)
  • Author(s): Daiki Yoshimatsu
  • Organizer: 日本生物物理学会第50回年回
  • Place of Presentation: 名古屋大学
• [Presentation] ミトコンドリアの一時的脱分極は活性酸素発生の抑制機構として働く (2011)
  • Author(s): 太田善浩、小野島匠
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-09-22
• [Presentation] Effects of cyclophilin D on energy metabolism of cells (2011)
  • Author(s): Osaki, H., Masuguchi, T., Nakazato, H., Matsunomoto, Y., Fujita, C., and Ohta, Y
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学
  • Year and Date: 2011-09-17
• [Presentation] Novel technique for study on the mechanism of mitochondrial volume maintenance (2011)
  • Author(s): Kanematsu K., Kaji, T., and Ohta, Y
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学
  • Year and Date: 2011-09-16
• [Presentation] Mitochondria reduce the production of ROS by their transient depolarizations (2011)
  • Author(s): Ohta Y
  • Organizer: The 8th Conference of Asian Society for Mitochondrial Research and Medicine
  • Place of Presentation: 鹿児島市民文化ホール
  • Year and Date: 2011-09-04
  • Invited
• [Presentation] Mitochondria reduce the production of ROS by their transient depoalrizations (2011)
  • Author(s): Ohta, Y, Onojima, T.
  • Organizer: 5th SFRE-Asia, 8th ASMRM, 11th J-mit
  • Place of Presentation: 鹿児島市民文化ホール(招待講演)
  • Year and Date: 2011-09-04
• [Presentation] An attempt to measure pH around mitochondria by using fluorescence microscopy with near field optical probe (2010)
  • Author(s): Haseda K., Li Y., Shinohara R., Kanezashi Y., Onojima T., Iwami K., Umeda N. and Ohta Y
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学
  • Year and Date: 2010-09-20
• [Presentation] An attempt to measure pH around mitochondria by using fluorescence microscopy with near field optical probe (2010)
  • Author(s): Haseda K., Li Y., Shinohara R., Kanezashi Y., Onojima T., Iwami K., Umeda N., Ohta Y.
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学, 仙台市
  • Year and Date: 2010-09-20
• [Presentation] Wavelength Fluorescent Ratiometric pH Measurement by Scanning Near-Field Optical Microscopy (2010)
  • Author(s): Li Y, Shinohara R., Iwami K., Ohta Y. and Umeda, N. Dual
  • Organizer: 6th International Symposium on Precision Engineering Measurements and Instrumentation (ISPEMI 2010)
  • Place of Presentation: Hangzhou, China
  • Year and Date: 2010-08-10
• [Presentation] Dual Wavelength Fluorescent Ratiometric pH Measurement by Scanning Near-Field Optical Microscopy (2010)
  • Author(s): Li Y, Shinohara R., Iwami K., Ohta Y., Umeda、N.
  • Organizer: 6th International Symposium on Precision Engineering Measurements and Instrumentation (ISPEMI 2010)
  • Place of Presentation: Hangzhou, China
  • Year and Date: 2010-08-10
• [Remarks] 太田研究室
  • URL: http://www.tuat.ac.jp/~ohta/
• [Remarks]
  • URL: http://www.tuat.ac.jp/~ohta/