Elucidation of the activation mechanismof photoactivated adenylyl cyclase (PAC)
| Project/Area Number |
22570159
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Toho University (2011-2012)
The Graduate University for Advanced Studies (2010) |
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Principal Investigator |
ISEKI Mineo 東邦大学, 薬学部, 准教授 (60414009)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 光センサー / アデニル酸シクラーゼ / cAMP / 光生物 / 光受容体 / フラビンタンパク質 / 硫黄細菌 / アデニル酸シクラ－ゼ / フラビン / ミドリムシ |
|---|
| Research Abstract |
This research is aimed at elucidating the activation mechanism of photoactivated adenylyl cyclase (PAC), thereby promoting biotechnological application of the enzyme. We demonstrated that a bacterial homolog of PAC from Beggiatoa(BsPAC) showed light dependent activity of adenylyl cyclase in E. coli. By analyzing the functions of BsPAC mutants, we identified several amino acid residues that have important roles for intramolecular signal transduction of the photoactivated enzyme
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Photomanipulation of antibiotic susceptibility and biofilm formation of <i>Escherichia coli</i> heterologously expressing photoactivated adenylyl cyclase2012
Author(s)
Yasukawa, H., Konno, N., Haneda, Y., Yamamori, B., Iseki, M., Shibusawa, M., Ono, Y., Kodaira, K., Funada, H. and Watanabe, M.
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Journal Title
The Journal of General and Applied Microbiology
Volume: 58
Issue: 3
Pages: 183-190
DOI
NAID
ISSN
0022-1260, 1349-8037
Related Report
Peer Reviewed
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[Journal Article] Blue and red light-induced germination of resting spores in the red-tide diatom Leptocylindrus danicus2011
Author(s)
Shikata, T., Iseki, M., Matsunaga, S., Higashi, S., Kamei, Y. and Watanabe, M
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Journal Title
Photochem Photobiol
Volume: 87
Issue: 3
Pages: 590-597
DOI
Related Report
Peer Reviewed
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[Journal Article] Differentiation of photocycle characteristics of flavin-binding BLUF domains of α-and β-subunits of photoactivated adenylyl cyclase of Euglena gracilis2010
Author(s)
Ito, S., Murakami, A., Iseki, M., Takahashi, T., Higashi, S. and Watanabe, M
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Journal Title
Photochem. Photobiol. Sci.
Volume: 9
Issue: 10
Pages: 1327-1335
DOI
Related Report
Peer Reviewed
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