Analysis of the components of a novel coactivator.the NF complex, and its regulatory mechanisms

• Project/Area Number: 22570168
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: University of Tsukuba
• Principal Investigator: HISATAKE Koji 筑波大学, 医学医療系, 教授 (70271236)
• Co-Investigator(Kenkyū-buntansha): FUKUDA Aya 筑波大学, 医学医療系, 准教授 (50436276)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 転写 / コアクチベーター / がん遺伝子 / RNA結合タンパク質 / クロマチン免疫沈降 / ガン遺伝子

Research Abstract

The transcriptional activities of the NF complexes (NF45-NF90 and NF45-NF110) are mediated by both the upstream enhancer and core promoter regions of the c-fos gene. Knockdown of the endogenous NF90/NF110 in mouse cells results in a diminished induction of c-fos transcription upon serum stimulation. Chromatin immunoprecipitation assays show that the NF complexes occupy the c-fos enhancer/promoter region before and after serum induction and that their occupancies within the coding region of the c-fos gene increase in parallel to that of RNAPII upon serum induction. Thus, the NF complexes appear to serve as multifunctional coactivators that coordinate different steps of gene expression to facilitate rapid response of inducible genes.

Research Products

• [Presentation] Application of Cytoplasmic RNA vector Developed from Noncytopathic RNA Viruses to Stem Cell Engineering (2013)
  • Author(s): 西村健
  • Organizer: The 2nd Meeting on RNA and Biofunctions-Asia Study
  • Place of Presentation: 福岡
• [Presentation] SeVdpベクターを用いたiPS細胞の品質調節のメカニズムの解析 (2013)
  • Author(s): 西村健
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜
• [Presentation] The RNA-binding protein complexes NF45/90 and NF45/110 activate c-fos transcription in a signal-dependent way (2012)
  • Author(s): Fukuda A, Nakadai T, Shimada M, Nishimura K, Hisatake K
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
  • Year and Date: 2012-12-02
• [Presentation] RNA工学とウイルス学の融合による簡便かつ高効率なiPS細胞樹立方法の確立 (2012)
  • Author(s): 西村健
  • Organizer: International Society of Stem Cell Research 10th Annual Meeting
  • Place of Presentation: 横浜
• [Presentation] The RNA-binding protein complexes NF45/90 and NF45/110 activate c-fos transcription in a signal-dependent way (2012)
  • Author(s): 福田綾
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] RNA工学とウイルス学の融合による簡便かつ高効率なiPS細胞樹立方法の確立 (2011)
  • Author(s): 西村健、大高真奈美、高安聡子、佐野将之、久武幸司、中西真人
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-19
• [Presentation] Heterogeneous nuclear ribonucleoprotein R (hnRNP R) cooperates with Mediator to enhance transcription reinitiation (2011)
  • Author(s): Fukuda A, Shimada M, Nakadai T, Nishimura K, Hisatake K
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-19
• [Presentation] Heterogeneous nuclear ribonucleoprotein R (hnRNP R) cooperates with Mediator to enhance transcription reinitiation (2011)
  • Author(s): Fukuda A, Shimada M, Nakadai T, Nishimura K, Hisatake K
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-15
• [Presentation] Activation of multiple-round transcriptions by a novel coactivator hnRNP R and Mediator (2011)
  • Author(s): Fukuda A, Takeuchi M, Nakadai T, Shimada M, Nishimura K, Hisatake K
  • Organizer: Cold Spring Harbor Laboratory meeting on "Mechanisms of Eukaryotic Transcription"
  • Place of Presentation: New York, USA
  • Year and Date: 2011-08-31
• [Presentation] R Novel Cytoplasmic persistent expression RNA vector developed by combination of RNA biotechnology and virology (2011)
  • Author(s): Nishimura K, Ohtaka M, Sano M, Hisatake K, Nakanishi M
  • Organizer: NTU-JST Joint Meeting on RNA and Biofunctions-Asia Studies
  • Place of Presentation: 台湾
• [Presentation] Mechanistic analyses of transcriptional regulation of the c-fos gene via the NF complexes (2010)
  • Author(s): Nakadai T, Fukuda A, Takeuchi M, Shimada M, Hisatake K
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会合同大会
  • Place of Presentation: 神戸国際会議場・神戸ポートピアホテル
  • Year and Date: 2010-12-07
• [Presentation] Mechanism of c-fos transcriptional regulation by an RNA-binding protein, hnRNP R (2010)
  • Author(s): Hisatake K, Takeuchi M, Nakadai T, Shimada M, Fukuda A
  • Organizer: Leading Graduate Schools International Conference
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2010-11-01
• [Presentation] Mechanism of c-fos transcriptional regulation by an RNA-binding protein, hnRNP R (2010)
  • Author(s): Hisatake K, Takeuchi M, Nakadai T, Shimada M, Fukuda A
  • Organizer: ASBMB Special Symposia "Transcription Regulation by Chromatin and RNA Polymerase II"
  • Place of Presentation: Granlibakken, Lake Tahoe, USA
  • Year and Date: 2010-10-02
• [Presentation] Mechanistic Analysis of c-fos Transcriptional Regulation by a Novel Coactivator hnRNP R (2010)
  • Author(s): Fukuda A, Takeuchi M, Nakadai T, Shimada M, Hisatake K
  • Organizer: 9th EMBL Conference "Transcription and Chromatin"
  • Place of Presentation: Heidelberg, Germany
  • Year and Date: 2010-08-29
• [Presentation] Mechanistic analysis of c-fos transcriptional regulation by a novel coactivator hnRNP R (2010)
  • Author(s): Fukuda A, Takeuchi M, Nakadai T, Shimada M, Hisatake K
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会合同大会
  • Place of Presentation: 神戸国際会議場・神戸ポートピアホテル
• [Remarks] 筑波大学医学医療系 遺伝子制御学研究室ホームページ
  • URL: http://www.md.tsukuba.ac.jp/basic-med/biochem/gene/