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Transcriptional regulation of HSF1 by RPA

Research Project

Project/Area Number 22570171
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionYamaguchi University

Principal Investigator

FUJIMOTO Mitsuaki  山口大学, 大学院・医学系研究科, 講師 (80359900)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsHSF1 / RPA / FACT / がん / 転写 / RNA Polymerase II
Research Abstract

Heat shock factor 1 (HSF1) plays roles in control and stressed conditions by gaining access to target elements, but mechanisms of HSF1 access are not well known in mammalian cells. I found the physical interaction between the wing motif of human HSF1 and replication protein A (RPA), which is involved in DNA metabolism. Depletion of RPA1 abolishes HSF1 access to the promoter of HSP70 in unstressed condition and delays its rapid activation in response to heat shock. The HSF1-RPA complex leads to preloading of RNA polymerase II and opens the chromatin structure by recruiting a histone chaperone, FACT. Furthermore, this interaction is required for melanoma cell proliferation.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (18 results)

All 2013 2012 2011 2010 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (9 results) Remarks (2 results)

  • [Journal Article] Glutamine protects intestinal barrier function of colon epithelial cells from ethanol by modulating Hsp70 expression2013

    • Author(s)
      Reiko Akagi
    • Journal Title

      Pharmacology

      Volume: 91 Pages: 104-111

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RPA Assists HSF1 Access to Nucleosomal DNA by Recruiting Histone Chaperone FACT2012

    • Author(s)
      Mitsuaki Fujimoto, Eiichi Takaki, Ryosuke Takii, Ke Tan, Ramachandran Prakasam, Naoki Hayashida, Shun-ichiro Iemura,Tohru Natsume, and Akira Nakai
    • Journal Title

      Mol. Cell

      Volume: 48 Pages: 182-194

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Assists HSF1 Access to Nucleosomal DNA by Recruiting Histone Chaperone FACT2012

    • Author(s)
      MItsuaki Fujimoto
    • Journal Title

      Molecular Cell

      Volume: 48 Pages: 182-194

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impaired hippocampal spinogenesis and neurogenesis and altered affective behavior in mice lacking heat shock factor 12011

    • Author(s)
      Shusaku Uchida, Kumiko Hara, Ayumi Kobayashi, Mitsuaki Fujimoto, Koji Otsuki, Hirotake Yamagata, Teruyuki Hobara, Naoko Abe, Fumihiro Higuchi, Tomohiko Shibata, Shunsuke Hasegawa, Satoshi Kida, Akira Nakai, and Yoshifumi Watanabe
    • Journal Title

      Proc. Natl. Acad. Sci. U.S.A

      Volume: 108 Pages: 1681-1686

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Heat shock factor 2 is required for maintaining proteostasis against febrile-range thermal stress and polyglutaramine aggregation2011

    • Author(s)
      新川豊英
    • Journal Title

      Molecular Biology of the Cell

      Volume: 22 Pages: 3571-3583

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The heat shock factor family and adaptation to proteotoxic stress2010

    • Author(s)
      藤本充章
    • Journal Title

      FEBS Journal

      Volume: 277 Pages: 4112-4125

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heat shock factor 1 ameliorates proteotoxicity in cooperation with the transcription factor NFAT2010

    • Author(s)
      林田直樹
    • Journal Title

      EMBO Journal

      Volume: 29 Pages: 3459-3469

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] HSF1-RPA 複合体は腫瘍形成に必要である2012

    • Author(s)
      藤本充章
    • Organizer
      第85回日本生化学会
    • Place of Presentation
      福岡(マリンメッセ福岡)
    • Year and Date
      2012-12-16
    • Related Report
      2012 Final Research Report
  • [Presentation] HSF1-RPA複合体は腫瘍形成に必要である2012

    • Author(s)
      藤本充章
    • Organizer
      日本生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡・福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] HSF1-RPA 複合体はヌクレオソーム構造をとる DNA にアクセスする2011

    • Author(s)
      藤本充章
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      横浜 (パシフィコ横浜)
    • Year and Date
      2011-12-16
    • Related Report
      2012 Final Research Report
  • [Presentation] HSF1-RPA複合体はヌクレオソーム構造をとるDNAにアクセスする2011

    • Author(s)
      藤本充章
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜・横浜
    • Year and Date
      2011-12-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] HSF1-RPA 複合体はHSP70遺伝子への RNA ポリメラーゼのプリロードに必要である2011

    • Author(s)
      藤本充章
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都(国立京都国際会館)
    • Year and Date
      2011-09-24
    • Related Report
      2012 Final Research Report
  • [Presentation] HSF1-RPA複合体はHSP70遺伝子へのRNAポリメラーゼのプリロードに必要である2011

    • Author(s)
      藤本充章
    • Organizer
      日本生化学会大会
    • Place of Presentation
      国立京都国際会館・京都
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] HSF1-RPA complex is required for the preloading of RNA polyraerase II on HSP70 promoter in mammalian cells2011

    • Author(s)
      藤本充章
    • Organizer
      Gordon Research Conference
    • Place of Presentation
      バルガ・イタリア
    • Year and Date
      2011-07-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] DNA 修復因子 RPA は HSF1 を介する Hsp70 誘導に必要である2010

    • Author(s)
      藤本充章
    • Organizer
      第33回日本分子生物学会大会・第83回日本生化学会大会
    • Place of Presentation
      神戸(神戸国際会議場)
    • Year and Date
      2010-12-10
    • Related Report
      2012 Final Research Report
  • [Presentation] DNA修復因子RPAはHSF1を介するHsp70誘導に必要である2010

    • Author(s)
      藤本充章
    • Organizer
      第33回日本分子生物学会大会・第83回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド(神戸市)
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://ds22.cc.yamaguchi-u.ac.jp/~seika2/

    • Related Report
      2012 Final Research Report
  • [Remarks] 山口大学大学院医学系研究科医化学分野 医学部生化学第二講座

    • URL

      http://ds22.cc.yamaguchi-u.ac.jp/~seika2/index-3.html

    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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