| Project/Area Number |
22570172
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Kyushu University |
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Principal Investigator |
YAMAMOTO Ken 九州大学, 生体防御医学研究所, 准教授 (60274528)
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| Co-Investigator(Kenkyū-buntansha) |
TAHIRA Tomoko 九州大学, 生体防御医学研究所, 講師 (50155230)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 遺伝子転写 / PCAF / リジンメチル化 / アセチル化 / 転写 / タンパク修飾 |
|---|
| Research Abstract |
There exist several methyltransferases targeting histone molecules (HMTase). We previously reported that one of HMTase, Set9, methylates multiple lysine residues of p300/CBP-associated factor (PCAF). To elucidate biological relevance of this PCAF methylation, we examined functional change of PCAF cased by methylation. We found that lysine methylation did not alter stability and self-acethylation of PCAF; however, its acethylation activity to P53 and histone H3 molecules was impaired by this modification. Our results implicate a novel transcription pathway including Set9 and PCAF via multiple lysine methylation.
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