| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
(I) I developed a system for the identification of proteins interacting with a given target protein, by site-specific incorporation of a photo-reactive amino acid into the target protein, in vivo photo-cross-linking to interacting proteins, and identification of thus captured proteins by comparative quantitative mass spectrometry analysis. A novel long chain photoreactive amino acid was alsosuccessfully incorporated into proteins, which enables wide-range photo-cross-linking of proteins.
(II) I showed how RAMP and calcitonin receptor-like receptor interact with each other on the membrane of living cells by X-ray crystal structure analysis and site-specific photo-cross-linking assay.
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