Defining the molecular mechanism of checkpoint recovery from DNA damage.

• Project/Area Number: 22570180
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: KONISHI Akimitsu 東京医科歯科大学, 難治疾患研究所, 講師 (50381877)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 細胞周期 / DNA損傷チェックポイント / 分子生物学 / ケミカルスクリーニング / 癌抑制 / DNA損傷

Research Abstract

This project aims to understand how cells recover from DNA damage and to define the therapeutic potential for anti-cancer drug. We identified the inhibitors for the DNA damage recovery processes and revealed their molecular mechanism. Moreover, our newly identified drugs could inhibit the tumor cell growth. These findings will contribute to develop a novel strategy against cancer.

Research Products

• [Journal Article] Involvement of beclin 1 in engulfment of apoptotic cells (2012)
  • Author(s): Konishi, A., Arakawa, S., Yue, Z., and Shimizu, S
  • Journal Title: J Biol Chem Volume: 287 Pages: 13919-13929
  • Peer Reviewed
• [Journal Article] Involvement of Beclin 1 in the engulfment of apoptotic cells (2012)
  • Author(s): Konishi A, Arakawa S, Yue Z, Shimizu S
  • Journal Title: J.Biol.Chem. Volume: (epub) Issue: 17 Pages: 13919-13929
  • DOI: 10.1074/jbc.m112.348375
  • Peer Reviewed
• [Journal Article] Involvement of JNK in the regulation of autophagic cell death (2010)
  • Author(s): Shimizu, S., Konishi, A., Nishida, Y., Mizuta, T., Nishina, H., Yamamoto, A., and Tsujimoto, Y
  • Journal Title: Oncogene 29 Pages: 2070-2082
  • Peer Reviewed
• [Journal Article] Involvement of JNK in the regulation of autophagic cell death. (2010)
  • Author(s): Shimizu S, Konishi A, Nishida Y, Mizuta T, Nishina H, Yamamoto A, Tsujimoto Y
  • Journal Title: Oncogene Volume: 29 Pages: 2070-2082
  • Peer Reviewed
• [Presentation] DNA損傷チェックポイントの回復機構 (2012)
  • Author(s): Konishi A
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] Checkpoint recovery from DNA damage (2012)
  • Author(s): Konishi A
  • Organizer: 日本癌学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2012-09-21
• [Presentation] テロメア結合蛋白TRF2 のコアヒストンとの相互作用による染色体末端安定化機構 (2012)
  • Author(s): 小西昭充 、清水重臣、Titia de Lange
  • Organizer: 染色体ワークショップ
  • Place of Presentation: 仙台
  • Year and Date: 2012-01-25
• [Presentation] テロメア結合蛋白TRF2のコアヒストンとの相互作用による染色体末端安定化機構 (2012)
  • Author(s): 小西昭充、清水重臣、Titia de Lange
  • Organizer: 染色体ワークショップ
  • Place of Presentation: 仙台
  • Year and Date: 2012-01-25
• [Presentation] Checkpoint recovery from DNA damage (2012)
  • Author(s): Konishi A
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: ホテルロイトン札幌（北海道）
  • Invited
• [Presentation] DNA損傷チェックポイントの回復機構 (2012)
  • Author(s): Konishi A
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場（福岡）
  • Invited
• [Presentation] TRF2BASIC DOMAIN INTERACTS WITH NUCLEOSOMAL HISTONES TO STABILIZE CHROMOSOME ENDS (2011)
  • Author(s): Konishi A, Shimizu S, de Lange T
  • Organizer: 分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] TRF2 BASIC DOMAIN INTERACTS WITH NUCLEOSOMAL HISTONES TO PROTECT CHROMOSOME ENDS (2011)
  • Author(s): Konishi A, Shimizu S, de Lange T
  • Organizer: 分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] TRF2 BASIC DOMAIN INTERACTS WITH NUCLEOSOMAL HISTONES TO STABILIZE CHROMOSOME ENDS (2011)
  • Author(s): 小西昭充、清水重臣、Titia de Lange
  • Organizer: 研究所ネットワーク国際シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2011-06-09
• [Presentation] TRF2 BASIC DOMAIN INTERACTS WITH NUCLEOSOMAL HISTONES TO PROTECT CHROMOSOME ENDS (2011)
  • Author(s): 小西昭充、清水重臣、Titia de Lange
  • Organizer: 研究所ネットワーク国際シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2011-06-09
• [Presentation] TRF2BASIC DOMAIN INTERACTS WITH NUCLEOSOMAL HISTONES TO STABILIZE CHROMOSOME ENDS (2010)
  • Author(s): Konishi A, Shimizu S, de Lange T
  • Organizer: 分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] TRF2 BASIC DOMAIN INTERACTS WITH NUCLEOSOMAL HISTONES TO STABILIZE CHROMOSOME ENDS (2010)
  • Author(s): Konishi A, Shimizu S, de Lange T
  • Organizer: 分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Checkpoint Recovery fromDNA damage (2010)
  • Author(s): Konishi A
  • Organizer: MEXT Priority ResearchProject 国際シンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2010-11-06
• [Presentation] オートファジー因子Beclin1によるアポトーシス細胞除去の制御 (2010)
  • Author(s): 小西昭充、荒川聡子、清水重臣
  • Organizer: 日本Cell Death学会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-07-31
• [Remarks]
  • URL: http://www.tmd.ac.jp/mri/pcb/index.html