Analysis of physiological role of SVIL for cytokinesis

• Project/Area Number: 22570182
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Nagoya University
• Principal Investigator: SENGA Takeshi 名古屋大学, 医学系研究科, 准教授 (80419431)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 細胞質分裂 / SVIL / PLK1 / ミオシン / Central spindle / セントラルスピンドル / 中央体 / 収縮環 / リン酸化 / Central Spindle / Midbody / モータータンパク質 / Supervillin

Research Abstract

We found that Supervillin (SVIL) was localized to the central spindle during cytokinesis. The localization was dependent on the phosphorylation of Ser238 of SVIL. Expression ofSVIL with the substitution of Ser238 (SVIL-S238A) to alanine inhibited progression of cytokinesis. Furthermore, aberrant ingression of cytokinetic furrow was observed in SVIL-S238A expressing cells. Our results show that phosphorylation of SVIL is essential for the completion of cytokinesis.

Research Products

• [Journal Article] ALX1 induces Snail expression to promote epithelial to mesenchymal transition and invasion of ovarian cancer cells. (2013)
  • Author(s): Yuan H, Kajiyama H, Ito S, Yoshikawa N, Hyodo T, Asano E, Hasegawa H, Maeda M, Shibata K, Hamaguchi M, Kikkawa F, Senga T
  • Journal Title: Cancer Research Volume: 73 Pages: 1581-90
  • Peer Reviewed
• [Journal Article] S100A10 is required for the organization of actin stress fibers and promotion of cell spreading (2013)
  • Author(s): Sayeed S, Asano E, Ito S, Ohno K, Hamaguchi M, Senga T
  • Journal Title: Mol. Cell. Biochem. Volume: 374 Pages: 105-11
  • Peer Reviewed
• [Journal Article] S100A10 is required for the organization of actin stress fibers and promotion of cell spreading. (2013)
  • Author(s): Shurovi Sayeed
  • Journal Title: Molecular and cellular biochemistry Volume: 374 Issue: 1-2 Pages: 105-11
  • DOI: 10.1007/s11010-012-1509-2
  • Peer Reviewed
• [Journal Article] Misshapen-like kinase 1 (MINK1) is a novel component of striatin interacting phosphatase and kinase (STRIPAK) and is required for the completion of cytokinesis (2012)
  • Author(s): Hyodo T, Ito S, Hasegawa H, Asano E, Maeda M, Urano T, Takahashi, M, Hamaguchi M, Senga T
  • Journal Title: J Biol Chem Volume: 287: (30) Pages: 25019-29
  • Peer Reviewed
• [Journal Article] Misshapen-like kinase 1 (MINK1) is a novel component of striatin interacting phosphatase and kinase (STRIPAK) and is required for the completion of cytokinesis. (2012)
  • Author(s): Toshinori Hyodo
  • Journal Title: Journal of Biological Chemistry Volume: 287 Issue: 30 Pages: 25019-29
  • DOI: 10.1074/jbc.m112.372342
  • Peer Reviewed
• [Journal Article] Role of palladin phosphorylation by extracellular signal-regulated kinase in cell migration (2011)
  • Author(s): Asano E, Maeda M, Hasegawa H, Ito S, Hyodo T, Yuan H, Takahashi M, Hamaguchi M, Senga T
  • Journal Title: PLoS One
  • Peer Reviewed
• [Journal Article] ARHGAP18, a GTPase-activating protein for RhoA, controls cell shape, spreading, and motility. (2011)
  • Author(s): Maeda M, Hasegawa H, Hyodo T, Ito S, Asano E, Yuang H, Funasaka K, Shimokata K, Hasegawa Y, Hamaguchi M, Senga T
  • Journal Title: Mol Biol Cell Volume: 22(20) Pages: 3840-52
  • Peer Reviewed
• [Journal Article] ARHGAP18, a GTPase-activating protein for RhoA, controls cell shape, spreading, and migration (2011)
  • Author(s): 千賀威
  • Journal Title: Molecular Biology of the Cell Volume: 20 Pages: 3840-3852
  • Peer Reviewed
• [Journal Article] Recovery of anoikis in Src-transformed cells and human breast cancer cells by restoration of SIRPα1/SHP-2 signaling system (2011)
  • Author(s): 千賀威
  • Journal Title: Cancer Research Volume: 71 Pages: 1229-1234
  • Peer Reviewed
• [Journal Article] The roles of two distinct regions of PINCH-1 in the regulation of cell attachment and spreading (2010)
  • Author(s): Ito S, Takahara Y, Hyodo T, Hasegawa H, Asano E, Hamaguchi M, Senga T
  • Journal Title: Mol Biol Cell Volume: 21(23) Pages: 4120-9
  • Peer Reviewed
• [Journal Article] Cas utilizes Nck2 to activate Cdc42 and regulate cell polarization during cell migration in response to wound healing. (2010)
  • Author(s): Funasaka K, Ito S, Hasegawa H, Goldberg GS, Hirooka Y, Goto H, Hamaguchi M, Senga T
  • Journal Title: FEBS J Volume: 277(17) Pages: 3502-13
  • Peer Reviewed
• [Presentation] Supervillinのリン酸化は細胞分裂時の収縮環形成を制御する (2012)
  • Author(s): 千賀 威
  • Organizer: 日本癌学会
  • Place of Presentation: 札幌
  • Year and Date: 2012-09-20
• [Presentation] Supervillinのリン酸化は細胞分裂時の収縮環形成を制御する。 (2012)
  • Author(s): 千賀 威
  • Organizer: 日本癌学会
  • Place of Presentation: ロイトン札幌 （札幌）
• [Presentation] 細胞接着におけるPINCH-1の役割 (2011)
  • Author(s): 千賀威
  • Organizer: 日本癌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-05
• [Presentation] ARHGAP18はアクチン細胞骨格形成に関与し、cell spreadingや細胞運動能を制御する (2011)
  • Author(s): 千賀威
  • Organizer: 日本癌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-04
• [Remarks] 研究室のホームページに研究結果、業績を載せている。