| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Myxococcus xanthus btkB gene is expressed mainly in the growth phase and early stages of fruiting body development. When cultured in nutrient medium at high temperature (37 °C), btkB mutant showed reduced maximum cell density as compared to the wild type. Also, phosphorylated BtkA is expressed late after starvation induction and early after glycerol induction, and BtkA is required for the formation of mature spores.BtkA and BtkB are involved in the synthesis of exopolysaccharide. On the other hand, Php3 showed the enzymatic characteristics of PP2C-type serine/threonine protein phosphatases, and is involved in spore formation. PhpA shares homology with PHP family, and dephosphorylates Tyr-phosphorylated BtkA and BtkB. This enzyme is also involved in the production of exopolysaccharide.
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