| Project/Area Number |
22570189
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Tottori University (2012-2013)
Fukushima Medical University (2010-2011) |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
WADA Ikuo 福島県立医科大学, 医学部, 教授 (40182969)
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| Project Period (FY) |
2010-04-01 – 2013-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | SNAREタンパク質 / ファゴサイトーシス / ファゴソーム / 膜融合 / マクロファージ / 生体防御 / FRET解析 |
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| Research Abstract |
Phagosome maturation in phagocytes is thought to be accomplished by membrane fusion of phagosome with other organelles, but the molecular mechanism in this process remains unknown.
In this study, we have found SNAP-23, a plasma membrane-localized SNARE, functions not only in phagocytosis but also in phagosome maturation processes such as production of reactive oxygen species and acidification within phagosomes and fusion of phagosome with lysosome. To trace a conformational change of SNAP-23 during fusion event, we established a single-molecule fluorescence resonance energy transfer system for SNAP-23. Using this system, we have found a forming SNARE complex with VAMP7 on phagosomal membrane and a novel complex including VAMP5 and syntaxin3 on plasma membrane. This suggests that SNAP-23 mediates the membrane fusion of phagosome with lysosome by forming a SNARE complex with VAMP7. Unfortunately, we have never found a positive function of VAMP5 in phagosome formation and maturation.
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