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MembraneinducedalterationsofstructureandsignaltransductionmechanisminthePIP3-bindingdomains

Research Project

Project/Area Number 22570191
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionUniversity of Hyogo

Principal Investigator

TUZI Satoru  兵庫県立大学, 大学院・生命理学研究科, 准教授 (60227387)

Co-Investigator(Kenkyū-buntansha) YAGISAWA Hitoshi  兵庫県立大学, 大学院・生命理学研究科, 准教授 (40192380)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords細胞内情報伝達 / 固体NMR 分光法 / PH ドメイン / 分光法 / シグナル伝達 / 膜表在性蛋白質 / 脂質二重膜 / 生物物理 / PHドメイン / SWAP-70 / PIP3 / 固体NMR / 膜結合性蛋白質 / 二次構造
Research Abstract

Conformations of the pleckstrin homology (PH) domain in solutionand at the membrane surface were examined by using solid-state NMR, circular dichroism(CD) and fluorescence spectroscopy. The PH domains form one of the largest domainfamilies known to regulate membrane association of host proteins. Drastic conformationalalterations including a transition of the secondary structure were detected for themembrane-bound PH domain of a signal transduction protein SWAP-70. Theseconformational alterations induced at the membrane surface are suggested to regulatefunctions of SWAP-70, such as intracellular relocalization in response to the cellularsignaling.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (20 results)

All 2012 2011 2010 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (14 results) Remarks (4 results)

  • [Journal Article] Membrane-induced alteration of thesecondary structure in the SWAP-70pleckstrin homology domain2012

    • Author(s)
      N. Tokuda, K. Kawai, Y. H. Lee (OsakaUniversity), T. Ikegami (OsakaUniversity), S. Yamaguchi, H. Yagisawa,Y. Fukui (National Health Research Institutes, Taiwan), and S. Tuzi
    • Journal Title

      J.Biochem.

      Volume: 151 Pages: 391-401

    • Related Report
      2012 Final Research Report
  • [Journal Article] Membrane-induced alteration of the secondary structure in the SWAP-70 pleckstrin homology domain2012

    • Author(s)
      Tokuda N, Kawai K, Lee YH, Ikegami T, Yamaguchi S, Yagisawa H, Fukui Y, Tuzi S
    • Journal Title

      J Biochem

      Volume: 151 Issue: 4 Pages: 391-401

    • DOI

      10.1093/jb/mvr146

    • NAID

      10031166078

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Conformationalproperties of the Def6 PH domain locatedat the lipid bilayer surface2012

    • Author(s)
      Naomi Tokuda, Michikazu Tanio,Katsuyuki Nishimura, Toshiyuki Kohno,Daisuke Yokogawa, Takahisa, Ikegamiand Satoru Tuzi
    • Organizer
      The 50thannual meeting of the biophysical societyof Japan
    • Place of Presentation
      愛知県
    • Related Report
      2012 Final Research Report
  • [Presentation] 脂質二重膜上におけるDef6 PHドメインの高次構造の解析2012

    • Author(s)
      徳田尚美
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋大学(愛知県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Changes in thesecondary structure of the SWAP-70 PHdomain induced at the lipid bilayersurface2011

    • Author(s)
      Satoru Tuzi, Naomi Tokuda, KatsuhisaKawai, Young-Ho Lee (OsakaUniversity), Takahisa Ikegami (OsakaUniversity), Yasuhisa Fukui (NationalHealth Research Institutes, Taiwan) andHitoshi Yagisawa
    • Organizer
      The 10th JBS BiofrontierSymposium: International SymposiumNew Aspects of Phospholipid Biology andMedicine 2011
    • Place of Presentation
      福岡県
    • Related Report
      2012 Final Research Report
  • [Presentation] solid-stateNMR study of structural alteration andfunction of the PH domain induced at thelipid bilayer surface2011

    • Author(s)
      Naomi Tokuda, Katsuhisa Kawai,Young-Ho Lee (Osaka University),Takahisa Ikegami, Hitoshi, Yagisawa(Osaka University), Yasuhisa Fukui(National Health Research Institutes,Taiwan), and Satoru Tuzi, A
    • Organizer
      The internationalsymposium on nuclear magneticresonance 2011: the 50th memorialannual meeting of the nuclear magneticresonance society of Japan
    • Place of Presentation
      神奈川県
    • Related Report
      2012 Final Research Report
  • [Presentation] Conformational alteration of SWAP-70PH domain induced at the membranesurface2011

    • Author(s)
      Naomi Tokuda, Katsuhisa Kawai,Young-Ho Lee (Osaka University),Takahisa Ikegami (Osaka University),Hitoshi, Yagisawa, Yasuhisa Fukui(National Health Research Institutes,Taiwan), and Satoru Tuzi
    • Organizer
      The 49th annual meeting of thebiophysical society Japan
    • Place of Presentation
      兵庫県
    • Related Report
      2012 Final Research Report
  • [Presentation] Changes in the secondary structure of the SWAP-70 PH domain induced at the lipid bilayer surface2011

    • Author(s)
      Satoru Tuzi
    • Organizer
      The 10^<th> JBS biofrontier symposium
    • Place of Presentation
      Hotel Luigans, (Fukuoka, Japan)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 脂質膜上において誘起されるPHドメインの構造変化および機能の解析2011

    • Author(s)
      徳田尚美
    • Organizer
      日本生物物理学会第49回年会
    • Place of Presentation
      兵庫県立大学姫路書写キャンパス(兵庫県)
    • Related Report
      2011 Annual Research Report
  • [Presentation] A solid-state NMR study of structural alteration and function of the PH domain induced at the lipid bilayer surface2011

    • Author(s)
      Naomi Tokuda
    • Organizer
      The international symposium on nuclear magnetic resonance 2011 : the 50^<th> memorial annual meeting of the nuclear magnetic resonance society of Japan
    • Place of Presentation
      Osanbashi hall (Yokohama, Japan)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Coformational alterations of the pleckstrin homology domains at the lipid bilayer surface2010

    • Author(s)
      辻暁
    • Organizer
      2010 International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      Honolulu, Hawaii
    • Year and Date
      2010-12-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 固体NMR分光法によるPHドメインの脂質膜表面における構造変化の解析2010

    • Author(s)
      徳田尚美
    • Organizer
      第83回日本生化学会大会
    • Place of Presentation
      兵庫県、神戸ポートアイランド
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] 固体NMRによる脂質膜平面において誘起されるSWAP-70 PHドメインの構造変化および機能の解析2010

    • Author(s)
      徳田尚美
    • Organizer
      第49回NMR討論会
    • Place of Presentation
      東京都、タワーホール船堀
    • Year and Date
      2010-11-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] Conformationalalterations of the pleckstrin homologydomains at the lipid bilayer surface2010

    • Author(s)
      S. Tuzi, N. Tokuda, K. Kawai, Y. Fukui(National Health Research Institutes,Taiwan), H. Yagisawa
    • Organizer
      2010International Chemical Congress ofPacific Basin Societies (Honolulu 2010)
    • Place of Presentation
      アメリカ合衆国、ハワイ
    • Related Report
      2012 Final Research Report
  • [Presentation] 固体NMR 分光法によるPH ドメインの脂質膜表面における構造変化の解析2010

    • Author(s)
      徳田尚美・八木澤仁・福井泰久(NationalHealth Research Institutes, Taiwan)・辻暁
    • Organizer
      第83回日本生化学会大会
    • Place of Presentation
      兵庫県
    • Related Report
      2012 Final Research Report
  • [Presentation] 固体NMR による脂質膜表面において誘起されるSWAP-70 PHドメインの構造変化および機能の解析2010

    • Author(s)
      徳田尚美・川合克久・八木澤仁・福井泰久(National Health Research Institutes,Taiwan)・辻暁
    • Organizer
      第49回NMR 討論会
    • Place of Presentation
      東京都
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.sci.u-hyogo.ac.jp/life/biophys2/index-j.html

    • Related Report
      2012 Final Research Report
  • [Remarks] Molecular Biophysics II

    • URL

      http://www.sci.u-hyogo.ac.jp/life/biophys2/index-j.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.sci.u-hyogo.ac.jp/life/biophys2/MolBiophys_II_main

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.sci.u-hyogo.ac.jp/life/biophys2/MolBiophys_II_main/

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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