| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Conformations of the pleckstrin homology (PH) domain in solutionand at the membrane surface were examined by using solid-state NMR, circular dichroism(CD) and fluorescence spectroscopy. The PH domains form one of the largest domainfamilies known to regulate membrane association of host proteins. Drastic conformationalalterations including a transition of the secondary structure were detected for themembrane-bound PH domain of a signal transduction protein SWAP-70. Theseconformational alterations induced at the membrane surface are suggested to regulatefunctions of SWAP-70, such as intracellular relocalization in response to the cellularsignaling.
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