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Research on the role of intercellular signaling induced by cell membrane disruption

Research Project

Project/Area Number 22570193
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionSt. Marianna University School of Medicine

Principal Investigator

TOGO Tatsuru  聖マリアンナ医科大学, 医学部, 講師 (40334247)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords膜修復 / 細胞間情報伝達 / CREB / 一酸化窒素 / ATP / プロテインキナーゼA / プロテインキナーゼC / PKG
Research Abstract

Resealing of a disrupted plasma membrane at the micron-diameter range requires Ca2+-regulated exocytosis. Repeated membrane disruptions reseal more quickly than the initial wound, and this potentiation of membrane resealing persists for at least 24 hours after the initial wound in a wounded cell. The present study demonstrates that membrane resealing is potentiated in both wounded and neighboring cells in MDCK cells. Wounding of cells induces CREB phosphorylation, not only in wounded cells, but also in neighboring cells. Inhibition of the nitric oxide (NO)/protein kinase G (PKG) signaling pathway suppresses CREB phosphorylation in neighboring cells, but not in wounded cells. The potentiation of membrane resealing in neighboring cells is suppressed if the NO/PKG pathway is inhibited during the initial wound. Together, these results suggest that the NO/PKG pathway stimulates CREB phosphorylation in neighboring cells so that subsequent cell membrane disruptions of the neighboring csreseal more quickly.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (3 results)

All 2012 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (2 results)

  • [Journal Article] Cell membranedisruption stimulates NO/PKG signalingand potentiates cell membrane repair inneighboring cells.2012

    • Author(s)
      Togo,T.
    • Journal Title

      PLoS ONE

      Volume: 7 Issue: 8 Pages: e42885-e42885

    • DOI

      10.1371/journal.pone.0042885

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Presentation] Cell membrane disruption of a single cell stimulates NO/PKG signaling and potentiates cell membrane resealing in neighboring cells.2012

    • Author(s)
      東郷建
    • Organizer
      第45回日本発生生物学会・第64回日本細胞生物学会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2012-05-31
    • Related Report
      2012 Final Research Report
  • [Presentation] Cell membrane disruption of a single cell stimulates NO/PKG signaling and potentiates cell membrane resealing in neighboring cells

    • Author(s)
      東郷 建
    • Organizer
      第45回日本発生生物学会・第64回日本細胞生物学会合同大会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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